United States
Germany
Japan
United Kingdom
China
COL5A2 (NM_000393) Human Tagged ORF Clone
CAT＃: RC213335
COL5A2 (Myc-DDK-tagged)-Human collagen, type V, alpha 2 (COL5A2)
ORF Plasmid: tGFP
"NM_000393" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 10,256.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EDSC; EDSCL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213335 representing NM_000393
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCAAACTGGGCGGAAGCAAGACCTCTCCTCATTCTTATTGTTTTATTAGGGCAATTTGTCTCAA TAAAAGCCCAGGAAGAAGACGAGGATGAAGGATATGGTGAAGAAATAGCCTGCACTCAGAATGGCCAGAT GTACTTAAACAGGGACATTTGGAAACCTGCCCCTTGTCAGATCTGTGTCTGTGACAATGGAGCCATTCTC TGTGACAAGATAGAATGCCAGGATGTGCTGGACTGTGCCGACCCTGTAACGCCCCCTGGGGAATGCTGTC CTGTCTGTTCACAAACACCTGGAGGTGGCAATACAAATTTTGGTAGAGGAAGAAAGGGACAAAAGGGAGA ACCAGGATTAGTGCCTGTTGTAACAGGCATACGTGGTCGTCCAGGACCGGCAGGACCTCCAGGATCACAG GGACCAAGAGGAGAGCGAGGGCCAAAAGGAAGACCTGGCCCTCGTGGACCTCAGGGAATTGATGGAGAAC CAGGTGTTCCTGGTCAACCTGGTGCTCCAGGACCTCCTGGACATCCGTCCCACCCAGGACCCGATGGCTT GAGCAGGCCGTTTTCAGCTCAAATGGCTGGGTTGGATGAAAAATCTGGACTTGGGAGTCAAGTAGGACTA ATGCCTGGCTCTGTGGGTCCTGTTGGCCCAAGGGGACCACAGGGTTTACAAGGACAGCAAGGTGGTGCAG GACCTACAGGACCTCCTGGTGAACCTGGTGATCCTGGACCAATGGGTCCGATTGGTTCACGTGGACCAGA GGGCCCTCCTGGTAAACCTGGGGAAGATGGTGAACCTGGCAGAAATGGAAATCCTGGTGAAGTGGGATTT GCAGGATCTCCGGGAGCTCGTGGATTTCCTGGGGCTCCTGGTCTTCCAGGTCTGAAGGGTCACCGAGGAC ACAAAGGTCTTGAAGGCCCTAAAGGTGAAGTTGGAGCACCTGGTTCCAAGGGTGAAGCTGGCCCCACTGG TCCAATGGGTGCCATGGGTCCTCTGGGTCCGAGGGGAATGCCAGGAGAGAGAGGGAGACTTGGGCCACAG GGTGCTCCTGGACAACGAGGTGCACATGGTATGCCTGGAAAACCTGGACCAATGGGTCCTCTTGGGATAC CAGGCTCTTCTGGTTTTCCAGGAAATCCTGGAATGAAGGGAGAAGCAGGTCCTACAGGGGCGCGAGGCCC TGAAGGTCCTCAGGGGCAGAGAGGTGAAACTGGGCCCCCAGGTCCAGTTGGCTCTCCAGGTCTTCCTGGT GCAATAGGAACTGATGGTACTCCTGGTGCCAAAGGCCCAACGGGCTCTCCGGGTACCTCTGGTCCTCCTG GCTCAGCAGGGCCTCCTGGATCTCCAGGACCTCAGGGTAGCACTGGTCCTCAGGGAATTCGAGGCCAACC GGGTGATCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGCCCAAAAGGGGAACCAGGGCCACATGGTATT CAGGGTCCGATAGGCCCACCCGGTGAAGAAGGCAAAAGAGGTCCCAGAGGTGACCCAGGAACAGTTGGTC CTCCAGGGCCAGTGGGAGAAAGGGGTGCTCCTGGCAATCGTGGTTTTCCAGGCTCTGATGGTTTACCTGG GCCAAAGGGTGCTCAAGGAGAACGGGGTCCTGTAGGTTCTTCAGGACCCAAAGGAAGCCAGGGGGATCCA GGACGTCCAGGGGAACCTGGGCTTCCAGGTGCTCGGGGTTTGACAGGAAATCCTGGTGTTCAAGGTCCTG AAGGAAAACTTGGACCTTTGGGTGCGCCAGGGGAAGATGGCCGTCCAGGTCCTCCAGGCTCCATAGGAAT CAGAGGGCAGCCCGGGAGCATGGGCCTTCCAGGCCCCAAAGGTAGCAGTGGTGACCCTGGGAAACCTGGA GAAGCAGGAAATGCTGGAGTTCCTGGGCAGAGGGGAGCTCCTGGAAAAGATGGTGAAGTTGGTCCTTCTG GTCCTGTGGGCCCGCCGGGTCTAGCTGGTGAAAGAGGAGAACAAGGACCTCCAGGCCCCACAGGTTTTCA GGGGCTTCCTGGTCCTCCAGGGCCTCCTGGAGAAGGTGGAAAACCAGGTGATCAAGGTGTTCCTGGAGAT CCCGGAGCAGTTGGCCCGTTAGGACCTAGAGGAGAACGAGGAAATCCTGGGGAAAGAGGAGAACCTGGGA TAACTGGACTCCCTGGTGAGAAGGGAATGGCTGGAGGACATGGTCCTGATGGCCCAAAAGGCAGTCCAGG TCCATCTGGGACCCCTGGAGATACAGGCCCACCAGGTCTTCAAGGTATGCCGGGAGAAAGAGGAATTGCA GGAACTCCTGGCCCCAAGGGTGACAGAGGTGGCATAGGAGAAAAAGGTGCTGAAGGCACAGCTGGAAATG ATGGTGCAAGAGGTCTTCCAGGTCCTTTGGGCCCTCCAGGTCCGGCAGGTCCTACTGGAGAAAAGGGTGA ACCTGGTCCTCGAGGTTTAGTTGGCCCTCCTGGCTCCCGGGGCAATCCTGGTTCTCGAGGTGAAAATGGG CCAACTGGAGCTGTTGGTTTTGCCGGACCCCAGGGTCCTGACGGACAGCCTGGAGTAAAAGGTGAACCTG GAGAGCCAGGACAGAAGGGAGATGCTGGTTCTCCTGGACCACAAGGTTTAGCAGGATCCCCTGGCCCTCA TGGTCCTAATGGTGTTCCTGGACTAAAAGGTGGTCGAGGAACCCAAGGTCCGCCTGGTGCTACAGGATTT CCTGGTTCTGCGGGCAGAGTTGGACCTCCAGGCCCTGCTGGAGCTCCAGGACCTGCGGGACCCCTAGGGG AACCCGGGAAGGAGGGACCTCCAGGTCTTCGTGGGGACCCTGGCTCTCATGGGCGTGTGGGAGATCGAGG ACCAGCTGGCCCCCCTGGTGGCCCAGGAGACAAAGGGGACCCAGGAGAAGATGGGCAACCTGGTCCAGAT GGCCCCCCTGGTCCAGCTGGAACGACCGGGCAGAGAGGAATTGTTGGCATGCCTGGGCAACGTGGAGAGA GAGGCATGCCCGGCCTACCAGGCCCAGCGGGAACACCAGGAAAAGTAGGACCAACTGGTGCAACAGGAGA TAAAGGTCCACCTGGACCTGTGGGGCCCCCAGGCTCCAATGGTCCTGTAGGGGAACCTGGACCAGAAGGT CCAGCTGGCAATGATGGTACCCCAGGACGGGATGGTGCTGTTGGAGAACGTGGTGATCGTGGAGACCCTG GGCCTGCAGGTCTGCCAGGCTCTCAGGGTGCCCCTGGAACTCCTGGCCCTGTGGGTGCTCCAGGAGATGC AGGACAAAGAGGAGATCCGGGTTCTCGGGGTCCTATAGGACCACCTGGTCGAGCTGGGAAACGTGGATTA CCTGGACCCCAAGGACCTCGTGGTGACAAAGGTGATCATGGAGACCGAGGCGACAGAGGTCAGAAGGGCC ACAGAGGCTTTACTGGTCTTCAGGGTCTTCCTGGCCCTCCTGGTCCAAATGGTGAACAAGGAAGTGCTGG AATCCCTGGACCATTTGGCCCAAGAGGTCCTCCAGGCCCAGTTGGTCCTTCAGGTAAAGAAGGAAACCCT GGGCCACTTGGGCCAATTGGACCTCCAGGTGTACGAGGCAGTGTAGGAGAAGCAGGACCTGAGGGCCCTC CTGGTGAGCCTGGCCCACCTGGCCCTCCGGGTCCCCCTGGCCACCTTACAGCTGCTCTTGGGGATATCAT GGGGCACTATGATGAAAGCATGCCAGATCCACTTCCTGAGTTTACTGAAGATCAGGCGGCTCCTGATGAC AAAAACAAAACGGACCCAGGGGTTCATGCTACCCTGAAGTCACTCAGTAGTCAGATTGAAACCATGCGCA GCCCCGATGGCTCGAAAAAGCACCCAGCCCGCACGTGTGATGACCTAAAGCTTTGCCATTCCGCAAAGCA GAGTGGTGAATACTGGATTGATCCTAACCAAGGATCTGTTGAAGATGCAATCAAAGTTTACTGCAACATG GAAACAGGAGAAACATGTATTTCAGCAAACCCATCCAGTGTACCACGTAAAACCTGGTGGGCCAGTAAAT CTCCTGACAATAAACCTGTTTGGTATGGTCTTGATATGAACAGAGGGTCTCAGTTCGCTTATGGAGACCA CCAATCACCTAATACAGCCATTACTCAGATGACTTTTTTGCGCCTTTTATCAAAAGAAGCCTCCCAGAAC ATCACTTACATCTGTAAAAACAGTGTAGGATACATGGACGATCAAGCTAAGAACCTCAAAAAAGCTGTGG TTCTCAAAGGGGCAAATGACTTAGATATCAAAGCAGAGGGAAATATTAGATTCCGGTATATCGTTCTTCA AGACACTTGCTCTAAGCGGAATGGAAATGTGGGCAAGACTGTCTTTGAATATAGAACACAGAATGTGGCA CGCTTGCCCATCATAGATCTTGCTCCTGTGGATGTTGGCGGCACAGACCAGGAATTCGGCGTTGAAATTG GGCCAGTTTGTTTTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213335 representing NM_000393
Red=Cloning site Green=Tags(s) MMANWAEARPLLILIVLLGQFVSIKAQEEDEDEGYGEEIACTQNGQMYLNRDIWKPAPCQICVCDNGAIL CDKIECQDVLDCADPVTPPGECCPVCSQTPGGGNTNFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQ GPRGERGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGLSRPFSAQMAGLDEKSGLGSQVGL MPGSVGPVGPRGPQGLQGQQGGAGPTGPPGEPGDPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNPGEVGF AGSPGARGFPGAPGLPGLKGHRGHKGLEGPKGEVGAPGSKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQ GAPGQRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPVGSPGLPG AIGTDGTPGAKGPTGSPGTSGPPGSAGPPGSPGPQGSTGPQGIRGQPGDPGVPGFKGEAGPKGEPGPHGI QGPIGPPGEEGKRGPRGDPGTVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGSQGDP GRPGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDPGKPG EAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGD PGAVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGSPGPSGTPGDTGPPGLQGMPGERGIA GTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENG PTGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPNGVPGLKGGRGTQGPPGATGF PGSAGRVGPPGPAGAPGPAGPLGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDPGEDGQPGPD GPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEG PAGNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGDPGSRGPIGPPGRAGKRGL PGPQGPRGDKGDHGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNP GPLGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDESMPDPLPEFTEDQAAPDD KNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHSAKQSGEYWIDPNQGSVEDAIKVYCNM ETGETCISANPSSVPRKTWWASKSPDNKPVWYGLDMNRGSQFAYGDHQSPNTAITQMTFLRLLSKEASQN ITYICKNSVGYMDDQAKNLKKAVVLKGANDLDIKAEGNIRFRYIVLQDTCSKRNGNVGKTVFEYRTQNVA RLPIIDLAPVDVGGTDQEFGVEIGPVCFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000393 |
| ORF Size | 4497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000393.3, NP_000384.2 |
| RefSeq Size | 6930 bp |
| RefSeq ORF | 4500 bp |
| Locus ID | 1290 |
| UniProt ID | P05997 |
| Domains | COLFI, VWC, Collagen |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| MW | 144.7 kDa |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213335L3 | Lenti ORF clone of Human collagen, type V, alpha 2 (COL5A2), Myc-DDK-tagged |
CNY 16,820.00 |
|
| RC213335L4 | Lenti ORF clone of Human collagen, type V, alpha 2 (COL5A2), mGFP tagged |
CNY 16,820.00 |
|
| RG213335 | COL5A2 (tGFP-tagged) - Human collagen, type V, alpha 2 (COL5A2) |
CNY 11,856.00 |
|
| SC119899 | COL5A2 (untagged)-Human collagen, type V, alpha 2 (COL5A2) |
CNY 15,248.00 |
|
| SC318638 | COL5A2 (untagged)-Human collagen, type V, alpha 2 (COL5A2) |
CNY 28,880.00 |
