United States
Germany
Japan
United Kingdom
China
Estrogen Receptor 1 (ESR1) (NM_000125) Human Tagged ORF Clone
CAT＃: RC213277
ESR1 (Myc-DDK-tagged)-Human estrogen receptor 1 (ESR1), transcript variant 1
ORF Plasmid: tGFP
"NM_000125" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,648.00
| Cited in 13 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ER; Era; ESR; ESRA; ESTRR; NR3A1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213277 representing NM_000125.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCATGACCCTCCACACCAAAGCATCTGGGATGGCCCTACTGCATCAGATCCAAGGGAACGAGCTG GAGCCCCTGAACCGTCCGCAGCTCAAGATCCCCCTGGAGCGGCCCCTGGGCGAGGTGTACCTGGACAGC AGCAAGCCCGCCGTGTACAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCGGCCGCCGCCAAC GCGCAGGTCTACGGTCAGACCGGCCTCCCCTACGGCCCCGGGTCTGAGGCTGCGGCGTTCGGCTCCAAC GGCCTGGGGGGTTTCCCCCCACTCAACAGCGTGTCTCCGAGCCCGCTGATGCTACTGCACCCGCCGCCG CAGCTGTCGCCTTTCCTGCAGCCCCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCCAGCGGC TACACGGTGCGCGAGGCCGGCCCGCCGGCATTCTACAGGCCAAATTCAGATAATCGACGCCAGGGTGGC AGAGAAAGATTGGCCAGTACCAATGACAAGGGAAGTATGGCTATGGAATCTGCCAAGGAGACTCGCTAC TGTGCAGTGTGCAATGACTATGCTTCAGGCTACCATTATGGAGTCTGGTCCTGTGAGGGCTGCAAGGCC TTCTTCAAGAGAAGTATTCAAGGACATAACGACTATATGTGTCCAGCCACCAACCAGTGCACCATTGAT AAAAACAGGAGGAAGAGCTGCCAGGCCTGCCGGCTCCGCAAATGCTACGAAGTGGGAATGATGAAAGGT GGGATACGAAAAGACCGAAGAGGAGGGAGAATGTTGAAACACAAGCGCCAGAGAGATGATGGGGAGGGC AGGGGTGAAGTGGGGTCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAA CGCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCT GAGCCCCCGATACTCTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTA CTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGGCTTTGTG GATTTGACCCTCCATGATCAGGTCCACCTTCTAGAATGTGCCTGGCTAGAGATCCTGATGATTGGTCTC GTCTGGCGCTCCATGGAGCACCCAGGGAAGCTACTGTTTGCTCCTAACTTGCTCTTGGACAGGAACCAG GGAAAATGTGTAGAGGGCATGGTGGAGATCTTCGACATGCTGCTGGCTACATCATCTCGGTTCCGCATG ATGAATCTGCAGGGAGAGGAGTTTGTGTGCCTCAAATCTATTATTTTGCTTAATTCTGGAGTGTACACA TTTCTGTCCAGCACCCTGAAGTCTCTGGAAGAGAAGGACCATATCCACCGAGTCCTGGACAAGATCACA GACACTTTGATCCACCTGATGGCCAAGGCAGGCCTGACCCTGCAGCAGCAGCACCAGCGGCTGGCCCAG CTCCTCCTCATCCTCTCCCACATCAGGCACATGAGTAACAAAGGCATGGAGCATCTGTACAGCATGAAG TGCAAGAACGTGGTGCCCCTCTATGACCTGCTGCTGGAGATGCTGGACGCCCACCGCCTACATGCGCCC ACTAGCCGTGGAGGGGCATCCGTGGAGGAGACGGACCAAAGCCACTTGGCCACTGCGGGCTCTACTTCA TCGCATTCCTTGCAAAAGTATTACATCACGGGGGAGGCAGAGGGTTTCCCTGCCACGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC213277
Blue=ORF Red=Cloning site Green=Tag(s) MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAAN AQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSG YTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKA FFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEG RGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGL LTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQ GKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKIT DTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAP TSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC213277 also available, TP313277 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000125 |
| ORF Size | 1785 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 6456 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 2099 |
| UniProt ID | P03372 |
| Domains | HOLI, Oest_recep, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| MW | 66.2 kDa |
| Gene Summary | This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain. [provided by RefSeq, Jul 2020] |
Citations (13)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The crosstalk between IGF-1R and ER-α in the proliferation and anti-inflammation of nucleus pulposus cells
,Chen, RS;Zhang, XB;Zhu, XT;Wang, CS;,
Eur Rev Med Pharmacol Sci
,PubMed ID 32572901
[Estrogen Receptor 1]
|
|
Receptor-binding affinities of bisphenol A and its next-generation analogs for human nuclear receptors
,Liu, X;Sakai, H;Nishigori, M;Suyama, K;Nawaji, T;Ikeda, S;Nishigouchi, M;Okada, H;Matsushima, A;Nose, T;Shimohigashi, M;Shimohigashi, Y;,
Toxicol. Appl. Pharmacol.
,PubMed ID 31195007
[Estrogen Receptor 1]
|
|
ERα upregulates the expression of long non-coding RNA LINC00472 which suppresses the phosphorylation of NF-κB in breast cancer
,Wang, Z;Katsaros, D;Biglia, N;Shen, Y;Loo, L;Yu, X;Lin, H;Fu, Y;Chu, WM;Fei, P;Ni, Y;Jia, W;Deng, X;Qian, B;Yu, H;,
Breast Cancer Res. Treat.
,PubMed ID 30830488
[Estrogen Receptor 1]
|
|
Polyfluorinated iodine alkanes regulated distinct breast cancer cell progression through binding with estrogen receptor alpha or beta isoforms
,Song, W;Liu, QS;Sun, Z;Yang, X;Zhou, Q;Jiang, G;,
Environ. Pollut.
,PubMed ID 29665550
[Estrogen Receptor 1]
|
|
Estrogen receptor regulates hormone-induced growth arrest in a luminal A like breast cancer model
,Haddon, L;Hu, S;Jabbari, H;Loney, B;Lichtensztejn, Z;Fahlman, R;Hitt, M;McManus, K;Dabbs, K;Mackey, J;Hugh, J;,
bioRxiv
[Estrogen Receptor 1]
|
|
ER alpha binding by transcription factors NFIB and YBX1 enables FGFR2 signalling to modulate estrogen responsiveness in breast cancer
,Campbell, TM;Castro, MA;deOliveira, KG;Ponder, BA;Meyer, KB;,
Cancer Res.2017
,PubMed ID 29180470
[Estrogen Receptor 1]
|
|
RIZ1 is regulated by estrogen and suppresses tumor progression in endometrial cancer
,Yang, T;Ren, C;Jiang, A;Yu, Z;Li, G;Wang, G;Zhang, Q;,
Biochem. Biophys. Res. Commun.
,PubMed ID 28528974
[Estrogen Receptor 1]
|
|
Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer
,Hamilton, DH;Griner, LM;Keller, JM;Hu, X;Southall, N;Marugan, J;David, JM;Ferrer, M;Palena, C;,
Clin. Cancer Res
,PubMed ID 27267852
[Estrogen Receptor 1]
|
|
Synthesis and structure-activity relationships of 1-benzylindane derivatives as selective agonists for estrogen receptor beta
,Yonekubo, S;Fushimi, N;Miyagi, T;Nakanishi, O;Katsuno, K;Ozawa, M;Handa, C;Furuya, N;Muranaka, H;,
Bioorg. Med. Chem.
,PubMed ID 27692995
[Estrogen Receptor 1]
|
|
Let-7c blocks estrogen-activated Wnt signaling in induction of self-renewal of breast cancer stem cells
,Sun, X;Xu, C;Tang, SC;Wang, J;Wang, H;Wang, P;Du, N;Qin, S;Li, G;Xu, S;Tao, Z;Liu, D;Ren, H;,
Cancer Gene Ther.
,PubMed ID 26987290
[Estrogen Receptor 1]
|
|
Estrogen receptor alpha inhibits RLR-mediated immune response via ubiquitinating TRAF3
,Wang, C;Huang, Y;Sheng, J;Huang, H;Zhou, J;,
Cell. Signal.
,PubMed ID 26186972
[Estrogen Receptor 1]
|
|
Estrogen receptor a enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner
,Cao, P;Feng, F;Dong, G;Yu, C;Feng, S;Song, E;Shi, G;Liang, Y;Liang, G;,
BMC Cancer
,PubMed ID 26122040
[Estrogen Receptor 1]
|
|
?9-Tetrahydrocannabinol Disrupts Estrogen-Signaling through Up-Regulation of Estrogen Receptor β (ERβ)
,null,
Chemical research in toxicology
,PubMed ID 23718638
[Estrogen Receptor 1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213277L1 | Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, Myc-DDK-tagged |
CNY 9,048.00 |
|
| RC213277L2 | Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, mGFP tagged |
CNY 9,048.00 |
|
| RC213277L3 | Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, Myc-DDK-tagged |
CNY 9,048.00 |
|
| RC213277L4 | Lenti ORF clone of Human estrogen receptor 1 (ESR1), transcript variant 1, mGFP tagged |
CNY 9,048.00 |
|
| RG213277 | ESR1 (tGFP-tagged) - Human estrogen receptor 1 (ESR1), transcript variant 1 |
CNY 4,370.00 |
|
| SC125287 | ESR1 (untagged)-Human estrogen receptor 1 (ESR1), transcript variant 1 |
CNY 6,656.00 |
其它Estrogen Receptor 1产品
