TRPC5 (NM_012471) Human Tagged ORF Clone
CAT#: RC213238
TRPC5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5)
ORF Plasmid: tGFP
"NM_012471" in other vectors (6)
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CNY 7,128.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PPP1R159; TRP5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213238 representing NM_012471
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAACTGTACTACAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTGCAAATTGTGA GGGCTGAGACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTGGAGAAGGGGGACTATGCCAC TGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAATGTTAACATCAACTGCATGGACCCCTTGGGC CGGAGTGCCCTGCTCATTGCCATTGAGAACGAGAACCTGGAGATCATGGAGCTACTGCTGAACCACAGCG TGTATGTGGGTGATGCATTGCTCTATGCCATACGCAAGGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAG CTACAGGCGGCCCAGCGGAGAGAAGCAGGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACA CCGGACATCACTCCCATCATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTGCTTGTCCAAA AACGGGTCACTATCCCACGGCCCCACCAGATCCGCTGCAACTGTGTGGAGTGTGTGTCTAGTTCAGAGGT AGACAGCCTGCGCCACTCTCGCTCCCGACTGAACATCTATAAGGCTCTGGCAAGCCCCTCACTCATTGCC TTATCAAGTGAGGACCCCATCCTAACTGCCTTCCGTCTGGGCTGGGAGCTCAAGGAGCTCAGCAAGGTGG AGAATGAGTTCAAGGCCGAGTATGAGGAGCTCTCTCAGCAGTGCAAGCTCTTTGCCAAAGACCTGCTGGA CCAAGCTCGGAGCTCCAGGGAACTGGAGATCATCCTCAACCATCGAGATGACCACAGTGAAGAGCTTGAC CCTCAGAAGTACCATGACCTGGCCAAGTTGAAGGTGGCAATCAAATACCACCAGAAAGAGTTTGTTGCTC AGCCCAACTGCCAACAGTTGCTTGCCACCCTGTGGTATGATGGCTTCCCTGGATGGCGGCGGAAACACTG GGTAGTCAAGCTTCTAACCTGCATGACCATTGGGTTCCTGTTTCCCATGCTGTCTATAGCCTACCTGATC TCACCCAGGAGCAACCTTGGGCTGTTCATCAAGAAACCCTTTATCAAGTTTATCTGCCACACAGCATCCT ATTTGACCTTCCTCTTTATGCTTCTCCTGGCTTCTCAGCACATTGTCAGGACAGACCTTCATGTACAGGG GCCTCCCCCAACTGTCGTGGAATGGATGATATTGCCTTGGGTTCTAGGTTTCATTTGGGGTGAGATTAAG GAAATGTGGGATGGTGGATTTACTGAATACATCCATGACTGGTGGAACCTGATGGATTTTGCAATGAACT CCCTCTACCTGGCAACTATTTCCCTGAAGATTGTGGCCTATGTCAAGTATAATGGTTCTCGTCCAAGGGA GGAATGGGAAATGTGGCACCCGACTCTGATTGCGGAAGCACTCTTCGCAATATCCAACATTTTAAGTTCG TTGCGTCTCATATCCCTGTTCACAGCCAACTCCCACTTAGGACCTCTGCAGATCTCTTTGGGACGCATGC TGCTTGATATCCTCAAATTCCTCTTTATCTACTGCCTGGTACTACTAGCTTTTGCCAATGGACTGAACCA GCTTTACTTCTATTATGAAACCAGAGCTATCGATGAGCCTAACAACTGCAAGGGGATCCGATGTGAGAAA CAGAACAATGCCTTCTCCACGCTCTTTGAGACTCTTCAGTCACTCTTCTGGTCTGTATTTGGCCTTTTAA ATCTATATGTCACCAATGTGAAAGCCAGACACGAATTCACCGAGTTTGTAGGAGCTACCATGTTTGGAAC ATACAATGTCATCTCCCTGGTAGTGCTGCTGAACATGCTGATTGCTATGATGAACAACTCCTATCAGCTT ATTGCCGATCATGCTGATATCGAGTGGAAGTTTGCAAGGACGAAGCTCTGGATGAGTTACTTTGATGAAG GTGGCACCTTGCCACCTCCTTTCAACATCATCCCCAGCCCCAAGTCATTTCTATACCTTGGTAACTGGTT CAACAACACCTTCTGCCCCAAAAGAGACCCTGACGGTAGACGGAGAAGGCGCAACTTGAGAAGTTTCACA GAACGCAATGCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGGAATTTAGTCAAAAGAT ATGTGGCTGCTATGATAAGAAATTCCAAAACACATGAGGGACTTACAGAAGAAAATTTTAAGGAATTAAA GCAAGACATCTCCAGCTTTCGGTATGAAGTGCTTGACCTCTTGGGAAATAGAAAACATCCAAGGAGCTTT TCCACTAGCAGCACTGAACTGTCTCAGAGAGACGATAATAATGATGGCAGTGGTGGGGCTCGGGCCAAAT CCAAGAGTGTCTCTTTTAATTTAGGCTGCAAGAAAAAGACTTGCCATGGGCCACCTCTCATCAGAACCAT GCCAAGGTCCAGTGGTGCCCAAGGAAAGTCAAAAGCTGAGTCATCAAGCAAACGCTCCTTCATGGGTCCT TCTCTCAAGAAACTGGGTCTCCTATTCTCCAAATTTAATGGTCATATGTCTGAACCCAGTTCAGAGCCAA TGTACACAATTTCTGATGGAATTGTTCAGCAGCACTGTATGTGGCAGGACATCAGATATTCTCAGATGGA GAAAGGGAAAGCAGAGGCCTGTTCTCAAAGTGAAATTAACCTCAGTGAGGTAGAATTAGGTGAAGTCCAG GGCGCTGCTCAGAGCAGTGAATGCCCTCTAGCCTGTTCCAGCTCTCTTCACTGTGCATCCAGCATCTGCT CCTCAAATTCTAAACTTTTAGACTCCTCAGAGGATGTATTTGAAACTTGGGGAGAGGCTTGTGACTTGCT CATGCACAAATGGGGTGATGGACAGGAAGAACAAGTTACAACTCGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213238 representing NM_012471
Red=Cloning site Green=Tags(s) MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLNAVEKGDYATVKQALQEAEIYYNVNINCMDPLG RSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRRPSGEKQVPTLMMDTQFSEFT PDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVECVSSSEVDSLRHSRSRLNIYKALASPSLIA LSSEDPILTAFRLGWELKELSKVENEFKAEYEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELD PQKYHDLAKLKVAIKYHQKEFVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLI SPRSNLGLFIKKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIK EMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIVAYVKYNGSRPREEWEMWHPTLIAEALFAISNILSS LRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETRAIDEPNNCKGIRCEK QNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQL IADHADIEWKFARTKLWMSYFDEGGTLPPPFNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRRNLRSFT ERNADSLIQNQHYQEVIRNLVKRYVAAMIRNSKTHEGLTEENFKELKQDISSFRYEVLDLLGNRKHPRSF STSSTELSQRDDNNDGSGGARAKSKSVSFNLGCKKKTCHGPPLIRTMPRSSGAQGKSKAESSSKRSFMGP SLKKLGLLFSKFNGHMSEPSSEPMYTISDGIVQQHCMWQDIRYSQMEKGKAEACSQSEINLSEVELGEVQ GAAQSSECPLACSSSLHCASSICSSNSKLLDSSEDVFETWGEACDLLMHKWGDGQEEQVTTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012471 |
ORF Size | 2919 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012471.2 |
RefSeq Size | 5839 bp |
RefSeq ORF | 2922 bp |
Locus ID | 7224 |
UniProt ID | Q9UL62 |
Domains | ANK, ion_trans |
Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
MW | 111.2 kDa |
Gene Summary | This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Transient receptor potential canonical 5 mediates inflammatory mechanical and spontaneous pain in mice
,Sadler, KE;Moehring, F;Shiers, SI;Laskowski, LJ;Mikesell, AR;Plautz, ZR;Brezinski, AN;Mecca, CM;Dussor, G;Price, TJ;McCorvy, JD;Stucky, CL;,
Science translational medicine
,PubMed ID 34039739
[TRPC5]
|
Transient receptor potential canonical 5 (TRPC5) mediates inflammatory mechanical pain
,Sadler, K;Moehring, F;Shiers, S;Laskowski, L;Mikesell, A;Plautz, Z;Mecca, C;Dussor, G;Price, T;McCorvy, J;Stucky, C;,
bioRxiv
[TRPC5]
|
Molecular Determinants of the Sensitivity to Gq/11-phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel
,Chen, X;Li, W;Riley, AM;Soliman, M;Chakraborty, S;Stamatkin, CW;Obukhov, AG;,
J. Biol. Chem
,PubMed ID 27920205
[TRPC5]
|
Englerin A Agonizes the TRPC4/C5 Cation Channels to Inhibit Tumor Cell Line Proliferation
,null,
PLoS ONE
,PubMed ID 26098886
[TRPC5]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213238L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), Myc-DDK-tagged |
CNY 9,528.00 |
|
RC213238L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), mGFP tagged |
CNY 5,890.00 |
|
RC213238L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC213238L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), mGFP tagged |
CNY 9,528.00 |
|
RG213238 | TRPC5 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5) |
CNY 8,728.00 |
|
SC310023 | TRPC5 (untagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5) |
CNY 7,136.00 |