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BSND (NM_057176) Human Tagged ORF Clone
CAT＃: RC213125
BSND (Myc-DDK-tagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)
ORF Plasmid: tGFP
"NM_057176" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BART; DFNB73 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213125 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACGAGAAGACCTTCCGGATCGGCTTCATTGTGCTGGGGCTTTTCCTGCTGGCCCTCGGTACGT TCCTCATGAGCCATGATCGGCCCCAGGTCTACGGCACCTTCTATGCCATGGGCAGCGTCATGGTGATCGG GGGCATCATCTGGAGCATGTGCCAGTGCTACCCCAAGATCACCTTCGTCCCTGCTGACTCTGACTTTCAA GGCATCCTCTCCCCAAAGGCCATGGGCCTGCTGGAGAATGGGCTTGCTGCCGAGATGAAGAGCCCCAGTC CCCAGCCGCCCTATGTAAGGCTGTGGGAGGAAGCCGCCTATGACCAGAGCCTGCCTGACTTCAGCCACAT CCAGATGAAAGTCATGAGCTACAGTGAGGACCACCGCTCCTTGCTGGCCCCTGAGATGGGGCAGCCGAAG CTGGGAACCAGTGATGGAGGAGAAGGTGGCCCTGGCGACGTTCAGGCCTGGATGGAGGCTGCCGTGGTCA TCCACAAGGGCTCAGACGAGAGTGAAGGGGAAAGACGCCTAACTCAGAGCTGGCCCGGCCCCCTGGCCTG TCCCCAGGGCCCTGCCCCCTTGGCTTCCTTCCAAGATGACCTGGACATGGACTCCAGTGAAGGCAGCAGC CCCAATGCATCTCCACATGACAGGGAGGAAGCTTGTTCCCCACAACAGGAACCTCAGGGCTGCAGGTGCC CGCTGGACCGCTTCCAAGACTTTGCCCTGATTGATGCCCCAACGTTGGAGGATGAGCCCCAAGAGGGGCA GCAGTGGGAAATAGCCCTGCCCAACAACTGGCAGCGGTACCCAAGGACAAAGGTGGAGGAGAAGGAGGCT TCGGACACAGGTGGGGAGGAACCTGAGAAGGAAGAGGAAGACCTGTACTATGGGCTGCCAGATGGAGCCG GGGACCTCCTCCCGGACAAGGAGCTGGGTTTTGAGCCTGACACCCAAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213125 protein sequence
Red=Cloning site Green=Tags(s) MADEKTFRIGFIVLGLFLLALGTFLMSHDRPQVYGTFYAMGSVMVIGGIIWSMCQCYPKITFVPADSDFQ GILSPKAMGLLENGLAAEMKSPSPQPPYVRLWEEAAYDQSLPDFSHIQMKVMSYSEDHRSLLAPEMGQPK LGTSDGGEGGPGDVQAWMEAAVVIHKGSDESEGERRLTQSWPGPLACPQGPAPLASFQDDLDMDSSEGSS PNASPHDREEACSPQQEPQGCRCPLDRFQDFALIDAPTLEDEPQEGQQWEIALPNNWQRYPRTKVEEKEA SDTGGEEPEKEEEDLYYGLPDGAGDLLPDKELGFEPDTQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_057176 |
| ORF Size | 960 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_057176.3 |
| RefSeq Size | 1396 bp |
| RefSeq ORF | 963 bp |
| Locus ID | 7809 |
| UniProt ID | Q8WZ55 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 35.2 kDa |
| Gene Summary | This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Modified HEK cells simulate DCT cells in their sensitivity and response to changes in extracellular K
,Murthy, M;O'Shaughnessy, KM;,
Physiol Rep
,PubMed ID 31762176
[BSND]
|
|
Functional severity of CLCNKB mutations correlates with phenotypes in patients with classic Bartter's syndrome
,null,
The Journal of Physiology
,PubMed ID 28555925
[BSND]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213125L3 | Lenti ORF clone of Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213125L4 | Lenti ORF clone of Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND), mGFP tagged |
CNY 5,890.00 |
|
| RG213125 | BSND (tGFP-tagged) - Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND) |
CNY 4,000.00 |
|
| SC305749 | BSND (untagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND) |
CNY 2,400.00 |
