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ALP (PDLIM3) (NM_014476) Human Tagged ORF Clone
CAT＃: RC212972
PDLIM3 (Myc-DDK-tagged)-Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1
ORF Plasmid: tGFP
"NM_014476" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212972 representing NM_014476
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCAGACGGTGATCCTCCCGGGCCCTGCGCCCTGGGGCTTCAGGCTCTCAGGGGGCATAGACTTCA ACCAGCCTTTGGTCATCACCAGGATTACACCAGGAAGCAAGGCGGCAGCTGCCAACCTGTGTCCTGGAGA TGTCATCCTGGCTATTGACGGCTTTGGGACAGAGTCCATGACTCATGCTGATGCGCAGGACAGGATTAAA GCAGCAGCTCACCAGCTGTGTCTCAAAATTGACAGGGGAGAAACTCACTTATGGTCTCCACAAGTATCTG AAGATGGGAAAGCCCATCCTTTCAAAATCAACTTAGAATCAGAACCACAGGACGGGAACTACTTTGAACA CAAGCATAATATTCGGCCCAAACCTTTCGTGATCCCGGGCCGAAGCAGTGGATGCAGCACTCCCTCCGGG ATTGACTGTGGCAGTGGACGCAGCACCCCTTCTTCTGTCAGTACTGTTAGTACCATTTGCCCAGGTGACT TGAAAGTTGCGGCTAAGCTGGCCCCTAACATTCCTTTGGAAATGGAACTTCCTGGTGTGAAGATTGTACA TGCTCAGTTTAATACACCTATGCAGTTGTACTCAGATGACAATATTATGGAAACACTCCAGGGTCAGGTT TCAACAGCCCTAGGGGAAACACCTTTGATGAGCGAGCCCACAGCCTCGGTGCCCCCCGAGTCGGACGTGT ACCGGATGCTCCACGACAATCGGAATGAGCCCACACAGCCTCGCCAGTCGGGCTCCTTCAGAGTGCTCCA GGGAATGGTGGACGATGGCTCTGATGACCGTCCGGCTGGAACGCGGAGTGTGAGAGCTCCGGTGACGAAA GTCCATGGCGGTTCAGGCGGGGCACAGAGGATGCCGCTCTGTGACAAATGTGGGAGTGGCATAGTTGGTG CTGTGGTGAAGGCGCGGGATAAGTACCGGCACCCTGAGTGCTTCGTGTGTGCCGACTGCAACCTCAACCT CAAGCAAAAGGGCTACTTCTTCATAGAAGGGGAGCTGTACTGCGAAACCCACGCAAGAGCCCGCACAAAG CCCCCAGAGGGCTATGACACGGTCACTCTGTATCCCAAAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212972 representing NM_014476
Red=Cloning site Green=Tags(s) MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIK AAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQDGNYFEHKHNIRPKPFVIPGRSSGCSTPSG IDCGSGRSTPSSVSTVSTICPGDLKVAAKLAPNIPLEMELPGVKIVHAQFNTPMQLYSDDNIMETLQGQV STALGETPLMSEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTK VHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTK PPEGYDTVTLYPKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014476 |
| ORF Size | 1092 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014476.6 |
| RefSeq Size | 1722 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 27295 |
| UniProt ID | Q53GG5 |
| Domains | PDZ, LIM, ZM |
| MW | 39.1 kDa |
| Gene Summary | The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. [provided by RefSeq, Apr 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212972L1 | Lenti ORF clone of Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC212972L2 | Lenti ORF clone of Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC212972L3 | Lenti ORF clone of Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212972L4 | Lenti ORF clone of Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212972 | PDLIM3 (tGFP-tagged) - Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1 |
CNY 5,256.00 |
|
| SC313141 | PDLIM3 (untagged)-Human PDZ and LIM domain 3 (PDLIM3), transcript variant 1 |
CNY 5,990.00 |
