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GPR7 (NPBWR1) (NM_005285) Human Tagged ORF Clone
CAT＃: RC212756
NPBWR1 (Myc-DDK-tagged)-Human neuropeptides B/W receptor 1 (NPBWR1)
ORF Plasmid: tGFP
"NM_005285" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212756 representing NM_005285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACGCCTCGTTCTCGGAGCCCTGGCCCGCCAACGCATCGGGCCCGGACCCGGCGCTGAGCTGCT CCAACGCGTCGACTCTGGCGCCGCTGCCGGCGCCGCTGGCGGTGGCTGTACCAGTTGTCTACGCGGTGAT CTGCGCCGTGGGTCTGGCGGGCAACTCCGCCGTGCTGTACGTGTTGCTGCGGGCGCCCCGCATGAAGACC GTCACCAACCTGTTCATCCTCAACCTGGCCATCGCCGACGAGCTCTTCACGCTGGTGCTGCCCATCAACA TCGCCGACTTCCTGCTGCGGCAGTGGCCCTTCGGGGAGCTCATGTGCAAGCTCATCGTGGCTATCGACCA GTACAACACCTTCTCCAGCCTCTACTTCCTCACCGTCATGAGCGCCGACCGCTACCTGGTGGTGTTGGCC ACTGCGGAGTCGCGCCGGGTGGCCGGCCGCACCTACAGCGCCGCGCGCGCGGTGAGCCTGGCCGTGTGGG GGATCGTCACACTCGTCGTGCTGCCCTTCGCAGTCTTCGCCCGGCTAGACGACGAGCAGGGCCGGCGCCA GTGCGTGCTAGTCTTTCCGCAGCCCGAGGCCTTCTGGTGGCGCGCGAGCCGCCTCTACACGCTCGTGCTG GGCTTCGCCATCCCCGTGTCCACCATCTGTGTCCTCTATACCACCCTGCTGTGCCGGCTGCATGCCATGC GGCTGGACAGCCACGCCAAGGCCCTGGAGCGCGCCAAGAAGCGGGTGACCTTCCTGGTGGTGGCAATCCT GGCGGTGTGCCTCCTCTGCTGGACGCCCTACCACCTGAGCACCGTGGTGGCGCTCACCACCGACCTCCCG CAGACGCCGCTGGTCATCGCTATCTCCTACTTCATCACCAGCCTGAGCTACGCCAACAGCTGCCTCAACC CCTTCCTCTACGCCTTCCTGGACGCCAGCTTCCGCAGGAACCTCCGCCAGCTGATAACTTGCCGCGCGGC AGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC212756 representing NM_005285
Red=Cloning site Green=Tags(s) MDNASFSEPWPANASGPDPALSCSNASTLAPLPAPLAVAVPVVYAVICAVGLAGNSAVLYVLLRAPRMKT VTNLFILNLAIADELFTLVLPINIADFLLRQWPFGELMCKLIVAIDQYNTFSSLYFLTVMSADRYLVVLA TAESRRVAGRTYSAARAVSLAVWGIVTLVVLPFAVFARLDDEQGRRQCVLVFPQPEAFWWRASRLYTLVL GFAIPVSTICVLYTTLLCRLHAMRLDSHAKALERAKKRVTFLVVAILAVCLLCWTPYHLSTVVALTTDLP QTPLVIAISYFITSLSYANSCLNPFLYAFLDASFRRNLRQLITCRAAA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005285 |
| ORF Size | 984 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005285.5 |
| RefSeq Size | 987 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 2831 |
| UniProt ID | P48145 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 35.9 kDa |
| Gene Summary | Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Modulation of CaV1. 2 calcium channel by neuropeptide W regulates vascular myogenic tone via G protein-coupled receptor 7
,Ji, L;Zhu, H;Chen, H;Fan, W;Chen, J;Chen, J;,
Journal of Hypertension
,PubMed ID 26536090
[GPR7]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212756L3 | Lenti ORF clone of Human neuropeptides B/W receptor 1 (NPBWR1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212756L4 | Lenti ORF clone of Human neuropeptides B/W receptor 1 (NPBWR1), mGFP tagged |
CNY 5,890.00 |
|
| RG212756 | NPBWR1 (tGFP-tagged) - Human neuropeptides B/W receptor 1 (NPBWR1) |
CNY 4,000.00 |
|
| SC116841 | NPBWR1 (untagged)-Human neuropeptides B/W receptor 1 (NPBWR1) |
CNY 2,400.00 |
