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NFAT4 (NFATC3) (NM_004555) Human Tagged ORF Clone
CAT＃: RC212523
NFATC3 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2
ORF Plasmid: tGFP
"NM_004555" in other vectors (4)
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CNY 7,440.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NF-AT4c; NFAT4; NFATX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212523 representing NM_004555
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACTGCAAACTGTGGCGCCCACGACGAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGC CGGCGCCGCCGCCCCCGGGCTCGCGGCCTGCAGATCTTGAGCCAGATGATTGTGCATCCATTTACATCTT TAATGTAGATCCACCTCCATCTACTTTAACCACACCACTTTGCTTACCACATCATGGATTACCGTCTCAC TCTTCTGTTTTGTCACCATCGTTTCAGCTCCAAAGTCACAAAAACTATGAAGGAACTTGTGAGATTCCTG AATCTAAATATAGCCCATTAGGTGGTCCCAAACCCTTTGAGTGCCCAAGTATTCAAATTACATCTATCTC TCCTAACTGTCATCAAGAATTAGATGCACATGAAGATGACCTACAGATAAATGACCCAGAACGGGAATTT TTGGAAAGGCCTTCTAGAGATCATCTCTATCTTCCTCTTGAGCCATCCTACCGGGAGTCTTCTCTTAGTC CTAGTCCTGCCAGCAGCATCTCTTCTAGGAGTTGGTTCTCTGATGCATCTTCTTGTGAATCGCTTTCACA TATTTATGATGATGTGGACTCAGAGTTGAATGAAGCTGCAGCCCGATTTACCCTTGGATCCCCTCTGACT TCTCCTGGTGGCTCTCCAGGGGGCTGCCCTGGAGAAGAAACTTGGCATCAACAGTATGGACTTGGACACT CATTATCACCCAGGCAATCTCCTTGCCACTCTCCTAGATCCAGTGTCACTGATGAGAATTGGCTGAGCCC CAGGCCAGCCTCAGGACCCTCATCAAGGCCCACATCCCCCTGTGGGAAACGGAGGCACTCCAGTGCTGAA GTTTGTTATGCTGGGTCCCTTTCACCCCATCACTCACCTGTTCCTTCACCTGGTCACTCCCCCAGGGGAA GTGTGACAGAAGATACGTGGCTCAATGCTTCTGTCCATGGTGGGTCAGGCCTTGGCCCTGCAGTTTTTCC ATTTCAGTACTGTGTAGAGACTGACATCCCTCTCAAAACAAGGAAAACTTCTGAAGATCAAGCTGCCATA CTACCAGGAAAATTAGAGCTGTGTTCAGATGACCAAGGGAGTTTATCACCAGCCCGGGAGACTTCAATAG ATGATGGCCTTGGATCTCAGTATCCTTTAAAGAAAGATTCATGTGGTGATCAGTTTCTTTCAGTTCCTTC ACCCTTTACCTGGAGCAAACCAAAGCCTGGCCACACCCCTATATTTCGCACATCTTCATTACCTCCACTA GACTGGCCTTTACCAGCTCATTTTGGACAATGTGAACTGAAAATAGAAGTGCAACCTAAAACTCATCATC GAGCCCATTATGAAACTGAAGGTAGCCGAGGGGCAGTAAAAGCATCTACTGGGGGACATCCTGTTGTGAA GCTCCTGGGCTATAACGAAAAGCCAATAAATCTACAAATGTTTATTGGGACAGCAGATGATCGATATTTA CGACCTCATGCATTTTACCAGGTGCATCGAATCACTGGGAAGACAGTCGCTACTGCAAGCCAAGAGATAA TAATTGCCAGTACAAAAGTTCTGGAAATTCCACTTCTTCCTGAAAATAATATGTCAGCCAGTATTGATTG TGCAGGTATTTTGAAACTCCGCAATTCAGATATAGAACTTCGAAAAGGAGAAACTGATATTGGCAGAAAG AATACTAGAGTACGACTTGTGTTTCGTGTACACATCCCACAGCCCAGTGGAAAAGTCCTTTCTCTGCAGA TAGCCTCTATACCCGTTGAGTGCTCCCAGCGGTCTGCTCAAGAACTTCCTCATATTGAGAAGTACAGTAT CAACAGTTGTTCTGTAAATGGAGGTCATGAAATGGTTGTGACTGGATCTAATTTTCTTCCAGAATCCAAA ATCATTTTTCTTGAAAAAGGACAAGATGGACGACCTCAGTGGGAGGTAGAAGGGAAGATAATCAGGGAAA AATGTCAAGGGGCTCACATTGTCCTTGAAGTTCCTCCATATCATAACCCAGCAGTTACAGCTGCAGTGCA GGTGCACTTTTATCTTTGCAATGGCAAGAGGAAAAAAAGCCAGTCTCAACGTTTTACTTATACACCAGTT TTGATGAAGCAAGAACACAGAGAAGAGATTGATTTGTCTTCAGTTCCATCTTTGCCTGTGCCTCATCCTG CTCAGACCCAGAGGCCTTCCTCTGATTCAGGGTGTTCACATGACAGTGTACTGTCAGGACAGAGAAGTTT GATTTGCTCCATCCCACAAACATATGCATCCATGGTGACCTCATCCCATCTGCCACAGTTGCAGTGTAGA GATGAGAGTGTTAGTAAAGAACAGCATATGATTCCTTCTCCAATTGTACACCAGCCTTTTCAAGTCACAC CAACACCTCCTGTGGGGTCTTCCTATCAGCCTATGCAAACTAATGTTGTGTACAATGGACCAACTTGTCT TCCTATTAATGCTGCCTCTAGTCAAGAATTTGATTCAGTTTTGTTTCAGCAGGATGCAACTCTTTCTGGT TTAGTGAATCTTGGCTGTCAACCACTGTCATCCATACCATTTCATTCTTCAAATTCAGGCTCAACAGGAC ATCTCTTAGCCCATACACCTCATTCTGTGCATACCCTGCCTCATCTGCAATCAATGGGATATCATTGTTC AAATACAGGACAAAGATCTCTTTCTTCTCCAGTGGCTGACCAGATTACAGGTCAGCCTTCGTCTCAGTTA CAACCTATTACATATGGTCCTTCACATTCAGGGTCTGCTACAACAGCTTCCCCAGCAGCTTCTCATCCCT TGGCTAGTTCACCGCTTTCTGGGCCACCATCTCCTCAGCTTCAGCCTATGCCTTACCAATCTCCTAGCTC AGGAACTGCCTCATCACCGTCTCCAGCCACCAGAATGCATTCTGGACAGCACTCAACTCAAGCACAAAGT ACGGGCCAGGGGGGTCTTTCTGCACCTTCATCCTTAATATGTCACAGTTTGTGTGATCCAGCGTCATTTC CACCTGATGGGGCAACTGTGAGCATTAAACCTGAACCAGAAGATCGAGAGCCTAACTTTGCAACCATTGG TCTGCAGGACATCACTTTAGATGATGACCAATTTATATCTGACTTGGAACACCAGCCATCAGGTTCAGCA GAGAAATGGCCTAACCACAGTGTGCTCTCATGTCCAGCTCCTTTCTGGAGAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212523 representing NM_004555
Red=Cloning site Green=Tags(s) MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSH SSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREF LERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPLT SPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSPCGKRRHSSAE VCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAI LPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPL DWPLPAHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYL RPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRK NTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESK IIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPV LMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCR DESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSG LVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVADQITGQPSSQL QPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQS TGQGGLSAPSSLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDDQFISDLEHQPSGSA EKWPNHSVLSCPAPFWRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004555 |
| ORF Size | 3204 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004555.4 |
| RefSeq Size | 4005 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 4775 |
| UniProt ID | Q12968 |
| Domains | RHD, IPT |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| MW | 115.1 kDa |
| Gene Summary | The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibition of nuclear factor of activated T cells (NFAT) c3 activation attenuates acute lung injury and pulmonary edema in murine models of sepsis
,Karpurapu, M;Lee, YG;Qian, Z;Wen, J;Ballinger, MN;Rusu, L;Chung, S;Deng, J;Qian, F;Reader, BF;Nirujogi, TS;Park, GY;Pei, D;Christman, JW;,
Oncotarget
,PubMed ID 29535830
[NFAT4]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212523L3 | Lenti ORF clone of Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2, Myc-DDK-tagged |
CNY 12,540.00 |
|
| RC212523L4 | Lenti ORF clone of Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2, mGFP tagged |
CNY 12,540.00 |
|
| RG212523 | NFATC3 (tGFP-tagged) - Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2 |
CNY 11,690.00 |
|
| SC117277 | NFATC3 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 (NFATC3), transcript variant 2 |
CNY 7,448.00 |
