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AMPD3 (NM_000480) Human Tagged ORF Clone
CAT＃: RC212077
AMPD3 (Myc-DDK-tagged)-Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 1
ORF Plasmid: tGFP
"NM_000480" in other vectors (4)
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CNY 5,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212077 representing NM_000480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGTCGTCCGAACCCGCTGAGATGCCGCGGCAGTTTCCCAAGCTGAACATCTCTGAAGTGGATG AGCAAGTCCGGCTCCTGGCGGAGAAGGTGTTTGCTAAAGTGCTCCGAGAAGAGGACAGCAAAGATGCCCT GTCCCTGTTCACTGTCCCAGAGGACTGCCCCATCGGGCAAAAGGAAGCCAAGGAGAGGGAGCTGCAGAAG GAGCTGGCAGAGCAGAAGTCTGTGGAGACCGCAAAAAGAAAGAAAAGTTTCAAGATGATTCGGTCCCAGT CCCTGTCTCTGCAAATGCCGCCACAGCAAGATTGGAAGGGCCCCCCGGCAGCCAGTCCGGCCATGTCTCC CACAACCCCTGTGGTCACTGGAGCCACTTCCCTGCCCACGCCAGCACCCTATGCCATGCCTGAGTTCCAG CGGGTCACCATCAGCGGAGATTACTGTGCCGGGATCACTTTGGAGGACTATGAGCAGGCAGCCAAGAGTC TGGCCAAGGCCCTAATGATCCGGGAGAAGTATGCGCGGCTCGCCTACCACCGCTTCCCGCGGATCACATC CCAGTACCTGGGTCATCCGCGGGCGGATACTGCACCTCCGGAAGAGGGCCTTCCAGACTTCCACCCTCCT CCACTGCCCCAGGAAGACCCCTACTGCCTGGATGATGCACCCCCCAACCTGGATTACTTGGTCCACATGC AGGGGGGCATCCTCTTTGTGTATGATAACAAGAAGATGCTGGAGCACCAGGAGCCGCACAGCCTACCCTA CCCCGACCTGGAGACCTACACGGTGGACATGAGCCACATCCTGGCTCTCATCACCGATGGCCCCACGAAA ACCTATTGTCACCGGCGACTGAACTTTCTGGAATCCAAGTTCAGCCTTCATGAGATGTTAAACGAAATGT CCGAGTTCAAAGAGTTGAAGAGTAACCCCCACCGGGACTTCTATAACGTGAGAAAGGTGGACACACACAT CCATGCGGCCGCCTGCATGAACCAAAAGCATCTGCTGCGCTTCATCAAGCACACATACCAGACGGAGCCT GACAGGACTGTGGCAGAGAAGCGGGGCCGGAAGATCACCCTGCGGCAGGTGTTTGACGGCCTGCACATGG ACCCCTACGACCTCACTGTGGACTCACTGGATGTCCACGCGGGCCGGCAGACATTCCACCGCTTTGACAA GTTCAACTCCAAATACAACCCTGTGGGGGCCAGTGAGCTGCGTGACCTGTATTTGAAAACTGAAAACTAT CTGGGAGGAGAGTACTTTGCTCGGATGGTCAAGGAGGTTGCCCGGGAGCTGGAGGAGAGCAAGTACCAGT ACTCAGAGCCACGGCTCTCCATCTACGGCCGCAGTCCTGAGGAGTGGCCCAACCTGGCCTACTGGTTCAT CCAGCACAAGGTCTACTCTCCCAACATGCGCTGGATCATCCAGGTGCCCCGGATTTATGACATATTTAGG TCAAAGAAGCTGCTGCCAAACTTTGGGAAGATGCTGGAGAACATCTTCCTGCCCCTTTTCAAGGCCACTA TCAACCCCCAAGATCATCGAGAGCTTCACCTCTTCCTTAAATATGTGACGGGGTTTGACAGCGTGGATGA TGAGTCCAAGCACAGCGACCACATGTTTTCCGACAAGAGCCCAAACCCGGACGTCTGGACCAGTGAGCAG AACCCACCCTACAGCTACTACCTGTACTACATGTATGCCAACATCATGGTGCTCAACAACCTCCGCAGGG AGCGCGGCCTGAGCACGTTCCTGTTCCGGCCGCACTGTGGGGAAGCCGGCTCCATCACCCACCTGGTGTC TGCCTTCCTCACTGCTGACAACATTTCCCACGGGCTGCTCCTCAAGAAGAGTCCGGTATTGCAGTATCTC TACTACCTTGCTCAGATCCCCATTGCCATGTCTCCTCTTAGCAACAACAGTTTGTTCCTCGAATATTCCA AGAACCCTCTGAGGGAATTCCTACACAAGGGACTGCATGTTTCTCTTTCCACCGATGACCCCATGCAGTT CCACTACACGAAGGAAGCACTTATGGAAGAATATGCCATTGCAGCTCAAGTGTGGAAGCTGAGCACCTGC GACCTGTGTGAGATCGCCAGGAACAGCGTGCTGCAGAGCGGCCTCTCGCATCAGGAAAAGCAAAAGTTTC TGGGACAAAATTATTATAAAGAAGGACCTGAAGGAAATGATATTCGAAAGACAAATGTGGCTCAGATCCG GATGGCATTCCGATATGAGACCTTATGCAATGAGCTCAGCTTCCTGTCTGATGCTATGAAATCAGAAGAG ATCACCGCCTTGACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212077 representing NM_000480
Red=Cloning site Green=Tags(s) MALSSEPAEMPRQFPKLNISEVDEQVRLLAEKVFAKVLREEDSKDALSLFTVPEDCPIGQKEAKERELQK ELAEQKSVETAKRKKSFKMIRSQSLSLQMPPQQDWKGPPAASPAMSPTTPVVTGATSLPTPAPYAMPEFQ RVTISGDYCAGITLEDYEQAAKSLAKALMIREKYARLAYHRFPRITSQYLGHPRADTAPPEEGLPDFHPP PLPQEDPYCLDDAPPNLDYLVHMQGGILFVYDNKKMLEHQEPHSLPYPDLETYTVDMSHILALITDGPTK TYCHRRLNFLESKFSLHEMLNEMSEFKELKSNPHRDFYNVRKVDTHIHAAACMNQKHLLRFIKHTYQTEP DRTVAEKRGRKITLRQVFDGLHMDPYDLTVDSLDVHAGRQTFHRFDKFNSKYNPVGASELRDLYLKTENY LGGEYFARMVKEVARELEESKYQYSEPRLSIYGRSPEEWPNLAYWFIQHKVYSPNMRWIIQVPRIYDIFR SKKLLPNFGKMLENIFLPLFKATINPQDHRELHLFLKYVTGFDSVDDESKHSDHMFSDKSPNPDVWTSEQ NPPYSYYLYYMYANIMVLNNLRRERGLSTFLFRPHCGEAGSITHLVSAFLTADNISHGLLLKKSPVLQYL YYLAQIPIAMSPLSNNSLFLEYSKNPLREFLHKGLHVSLSTDDPMQFHYTKEALMEEYAIAAQVWKLSTC DLCEIARNSVLQSGLSHQEKQKFLGQNYYKEGPEGNDIRKTNVAQIRMAFRYETLCNELSFLSDAMKSEE ITALTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000480 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000480.3 |
| RefSeq Size | 4371 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 272 |
| UniProt ID | Q01432 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| MW | 89.5 kDa |
| Gene Summary | This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212077L3 | Lenti ORF clone of Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212077L4 | Lenti ORF clone of Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212077 | AMPD3 (tGFP-tagged) - Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 1 |
CNY 4,370.00 |
|
| SC300076 | AMPD3 (untagged)-Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 1 |
CNY 7,220.00 |
