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NEK7 (NM_133494) Human Tagged ORF Clone
CAT＃: RC211857
NEK7 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7)
ORF Plasmid: tGFP
"NM_133494" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211857 representing NM_133494
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTCCTCAGTTCCAACCACAGAAGGCCTTACGAC CGGATATGGGCTATAATACATTAGCCAACTTTCGAATAGAAAAGAAAATTGGTCGCGGACAATTTAGTGA AGTTTATAGAGCAGCCTGTCTCTTGGATGGAGTACCAGTAGCTTTAAAAAAAGTGCAGATATTTGATTTA ATGGATGCCAAAGCACGTGCTGATTGCATCAAAGAAATAGATCTTCTTAAGCAACTCAACCATCCAAATG TAATAAAATATTATGCATCATTCATTGAAGATAATGAACTAAACATAGTTTTGGAACTAGCAGATGCTGG CGACCTATCCAGAATGATCAAGCATTTTAAGAAGCAAAAGAGGCTAATTCCTGAAAGAACTGTTTGGAAG TATTTTGTTCAGCTTTGCAGTGCATTGGAACACATGCATTCTCGAAGAGTCATGCATAGAGATATAAAAC CAGCTAATGTGTTCATTACAGCCACTGGGGTGGTAAAACTTGGAGATCTTGGGCTTGGCCGGTTTTTCAG CTCAAAAACCACAGCTGCACATTCTTTAGTTGGTACGCCTTATTACATGTCTCCAGAGAGAATACATGAA AATGGATACAACTTCAAATCTGACATCTGGTCTCTTGGCTGTCTACTATATGAGATGGCTGCATTACAAA GTCCTTTCTATGGTGACAAAATGAATTTATACTCACTGTGTAAGAAGATAGAACAGTGTGACTACCCACC TCTTCCTTCAGATCACTATTCAGAAGAACTCCGACAGTTAGTTAATATGTGCATCAACCCAGATCCAGAG AAGCGACCAGACGTCACCTATGTTTATGACGTAGCAAAGAGGATGCATGCATGCACTGCAAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211857 representing NM_133494
Red=Cloning site Green=Tags(s) MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDL MDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWK YFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHE NGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPE KRPDVTYVYDVAKRMHACTASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133494 |
| ORF Size | 906 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133494.3 |
| RefSeq Size | 1072 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 140609 |
| UniProt ID | Q8TDX7 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 34.4 kDa |
| Gene Summary | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211857L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC211857L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), mGFP tagged |
CNY 5,890.00 |
|
| RC211857L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211857L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), mGFP tagged |
CNY 4,800.00 |
|
| RG211857 | NEK7 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 4,000.00 |
|
| SC120526 | NEK7 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 2,400.00 |
|
| SC323481 | NEK7 (untagged)-Kinase deficient mutant (K63M) of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 2,400.00 |
