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ADAMTS3 (NM_014243) Human Tagged ORF Clone
CAT＃: RC211602
- TrueORF®
ADAMTS3 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3)
ORF Plasmid: tGFP
"NM_014243" in other vectors (6)
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CNY 12,584.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADAMTS-4; HKLLS3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211602 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTCCTGTCACTTTGGTTGATAGCAGCCGCTCTGGTAGAGGTTAGGACTTCAGCTGATGGACAAG CTGGTAATGAAGAAATGGTGCAAATAGATTTACCAATAAAGAGATATAGAGAGTATGAGCTGGTGACTCC AGTCAGCACAAATCTAGAAGGACGCTATCTCTCCCATACTCTTTCTGCGAGTCACAAAAAGAGGTCAGCG AGGGACGTGTCTTCCAACCCTGAGCAGTTGTTCTTTAACATCACGGCATTTGGAAAAGATTTTCATCTGC GACTAAAGCCCAACACTCAACTAGTAGCTCCTGGGGCTGTTGTGGAGTGGCATGAGACATCTCTGGTGCC TGGGAATATAACCGATCCCATTAACAACCATCAACCAGGAAGTGCTACGTATAGAATCCGGAAAACAGAG CCTTTGCAGACTAACTGTGCTTATGTTGGTGACATCGTGGACATTCCAGGAACCTCTGTTGCCATCAGCA ACTGTGATGGTCTGGCTGGAATGATAAAAAGTGATAATGAAGAGTATTTCATTGAACCCTTGGAAAGAGG TAAACAGATGGAGGAAGAAAAAGGAAGGATTCATGTTGTCTACAAGAGATCAGCTGTAGAACAGGCTCCC ATAGACATGTCCAAAGACTTCCACTACAGAGAGTCGGACCTGGAAGGCCTTGATGATCTAGGTACTGTTT ATGGCAACATCCACCAGCAGCTGAATGAAACAATGAGACGCCGCAGACACGCGGGAGAAAACGATTACAA TATCGAGGTACTGCTGGGAGTGGATGACTCTGTGGTCCGTTTCCATGGCAAAGAGCACGTCCAAAACTAC CTCCTGACCCTAATGAACATTGTGAATGAAATTTACCATGATGAGTCCCTCGGAGTGCATATAAATGTGG TCCTGGTGCGCATGATAATGCTGGGATATGCAAAGTCCATCAGCCTCATAGAAAGGGGAAACCCATCCAG AAGCTTGGAGAATGTGTGTCGCTGGGCGTCCCAACAGCAAAGATCTGATCTCAACCACTCTGAACACCAT GACCATGCAATTTTTTTAACCAGGCAAGACTTTGGACCTGCTGGAATGCAAGGATATGCTCCAGTCACCG GCATGTGTCATCCAGTGAGAAGTTGTACCCTGAATCATGAGGATGGTTTTTCATCTGCTTTTGTAGTAGC CCATGAAACGGGCCATGTGTTGGGAATGGAGCATGATGGACAAGGCAACAGGTGTGGTGATGAGACTGCT ATGGGAAGTGTCATGGCTCCCTTGGTACAAGCAGCATTCCATCGTTACCACTGGTCCCGATGCAGTGGTC AAGAACTGAAAAGATATATCCATTCCTATGACTGTCTCCTTGATGACCCTTTTGATCATGATTGGCCTAA ACTCCCAGAACTTCCTGGAATCAATTATTCTATGGATGAGCAATGTCGTTTTGATTTTGGTGTTGGCTAT AAAATGTGCACCGCGTTCCGAACCTTTGACCCATGTAAACAGCTGTGGTGTAGCCATCCTGATAATCCCT ACTTTTGTAAGACTAAAAAGGGACCTCCACTTGATGGGACTGAATGTGCTGCTGGAAAATGGTGCTATAA GGGTCATTGCATGTGGAAGAATGCTAATCAGCAAAAACAAGATGGCAATTGGGGGTCATGGACTAAATTT GGCTCCTGTTCTCGGACATGTGGAACTGGTGTTCGTTTCAGAACACGCCAGTGCAATAATCCCATGCCCA TCAATGGTGGTCAGGATTGTCCTGGTGTTAATTTTGAGTACCAGCTTTGTAACACAGAAGAATGCCAAAA ACACTTTGAGGACTTCAGAGCACAGCAGTGTCAGCAGCGAAACTCCCACTTTGAATACCAGAATACCAAA CACCACTGGTTGCCATATGAACATCCTGACCCCAAGAAAAGATGCCACCTTTACTGTCAGTCCAAGGAGA CTGGAGATGTTGCTTACATGAAACAACTGGTGCATGATGGAACGCACTGTTCTTACAAAGATCCATATAG CATATGTGTGCGAGGAGAGTGTGTGAAAGTGGGCTGTGATAAAGAAATTGGTTCTAATAAGGTTGAGGAT AAGTGTGGTGTCTGTGGAGGAGATAATTCCCACTGCCGAACCGTGAAGGGGACATTTACCAGAACTCCCA GGAAGCTTGGGTACCTTAAGATGTTTGATATACCCCCTGGGGCTAGACATGTGTTAATCCAAGAAGACGA GGCTTCTCCTCATATTCTTGCTATTAAGAACCAGGCTACAGGCCATTATATTTTAAATGGCAAAGGGGAG GAAGCCAAGTCGCGGACCTTCATAGATCTTGGTGTGGAGTGGGATTATAACATTGAAGATGACATTGAAA GTCTTCACACCGATGGACCTTTACATGATCCTGTTATTGTTTTGATTATACCTCAAGAAAATGATACCCG CTCTAGCCTGACATATAAGTACATCATCCATGAAGACTCTGTACCTACAATCAACAGCAACAATGTCATC CAGGAAGAATTAGATACTTTTGAGTGGGCTTTGAAGAGCTGGTCTCAGTGTTCCAAACCCTGTGGTGGAG GTTTCCAGTACACTAAATATGGATGCCGTAGGAAAAGTGATAATAAAATGGTCCATCGCAGCTTCTGTGA GGCCAACAAAAAGCCGAAACCTATTAGACGAATGTGCAATATTCAAGAGTGTACACATCCACTCTGGGTA GCAGAAGAATGGGAACACTGCACCAAAACCTGTGGAAGTTCTGGCTATCAGCTTCGCACTGTACGCTGCC TTCAGCCACTCCTTGATGGCACCAACCGCTCTGTGCACAGCAAATACTGCATGGGTGACCGTCCCGAGAG CCGCCGGCCCTGTAACAGAGTGCCCTGCCCTGCACAGTGGAAAACAGGACCCTGGAGTGAGTGTTCAGTG ACCTGCGGTGAAGGAACGGAGGTGAGGCAGGTCCTCTGCAGGGCTGGGGACCACTGTGATGGTGAAAAGC CTGAGTCGGTCAGAGCCTGTCAACTGCCTCCTTGTAATGATGAACCATGTTTGGGAGACAAGTCCATATT CTGTCAAATGGAAGTGTTGGCACGATACTGCTCCATACCAGGTTATAACAAGTTATGTTGTGAGTCCTGC AGCAAGCGCAGTAGCACCCTGCCACCACCATACCTTCTAGAAGCTGCTGAAACTCATGATGATGTCATCT CTAACCCTAGTGACCTCCCTAGATCTCTAGTGATGCCTACATCTTTGGTTCCTTATCATTCAGAGACCCC TGCAAAGAAGATGTCTTTGAGTAGCATCTCTTCAGTGGGAGGTCCAAATGCATATGCTGCTTTCAGGCCA AACAGTAAACCTGATGGTGCTAATTTACGCCAGAGGAGTGCTCAGCAAGCAGGAAGTAAGACTGTGAGAC TGGTCACCGTACCATCCTCCCCACCCACCAAGAGGGTCCACCTCAGTTCAGCTTCACAAATGGCTGCTGC TTCCTTCTTTGCAGCCAGTGATTCAATAGGTGCTTCTTCTCAGGCAAGAACCTCAAAGAAAGATGGAAAG ATCATTGACAACAGACGTCCGACAAGATCATCCACCTTAGAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211602 protein sequence
Red=Cloning site Green=Tags(s) MVLLSLWLIAAALVEVRTSADGQAGNEEMVQIDLPIKRYREYELVTPVSTNLEGRYLSHTLSASHKKRSA RDVSSNPEQLFFNITAFGKDFHLRLKPNTQLVAPGAVVEWHETSLVPGNITDPINNHQPGSATYRIRKTE PLQTNCAYVGDIVDIPGTSVAISNCDGLAGMIKSDNEEYFIEPLERGKQMEEEKGRIHVVYKRSAVEQAP IDMSKDFHYRESDLEGLDDLGTVYGNIHQQLNETMRRRRHAGENDYNIEVLLGVDDSVVRFHGKEHVQNY LLTLMNIVNEIYHDESLGVHINVVLVRMIMLGYAKSISLIERGNPSRSLENVCRWASQQQRSDLNHSEHH DHAIFLTRQDFGPAGMQGYAPVTGMCHPVRSCTLNHEDGFSSAFVVAHETGHVLGMEHDGQGNRCGDETA MGSVMAPLVQAAFHRYHWSRCSGQELKRYIHSYDCLLDDPFDHDWPKLPELPGINYSMDEQCRFDFGVGY KMCTAFRTFDPCKQLWCSHPDNPYFCKTKKGPPLDGTECAAGKWCYKGHCMWKNANQQKQDGNWGSWTKF GSCSRTCGTGVRFRTRQCNNPMPINGGQDCPGVNFEYQLCNTEECQKHFEDFRAQQCQQRNSHFEYQNTK HHWLPYEHPDPKKRCHLYCQSKETGDVAYMKQLVHDGTHCSYKDPYSICVRGECVKVGCDKEIGSNKVED KCGVCGGDNSHCRTVKGTFTRTPRKLGYLKMFDIPPGARHVLIQEDEASPHILAIKNQATGHYILNGKGE EAKSRTFIDLGVEWDYNIEDDIESLHTDGPLHDPVIVLIIPQENDTRSSLTYKYIIHEDSVPTINSNNVI QEELDTFEWALKSWSQCSKPCGGGFQYTKYGCRRKSDNKMVHRSFCEANKKPKPIRRMCNIQECTHPLWV AEEWEHCTKTCGSSGYQLRTVRCLQPLLDGTNRSVHSKYCMGDRPESRRPCNRVPCPAQWKTGPWSECSV TCGEGTEVRQVLCRAGDHCDGEKPESVRACQLPPCNDEPCLGDKSIFCQMEVLARYCSIPGYNKLCCESC SKRSSTLPPPYLLEAAETHDDVISNPSDLPRSLVMPTSLVPYHSETPAKKMSLSSISSVGGPNAYAAFRP NSKPDGANLRQRSAQQAGSKTVRLVTVPSSPPTKRVHLSSASQMAAASFFAASDSIGASSQARTSKKDGK IIDNRRPTRSSTLER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014243 |
| ORF Size | 3615 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014243.1, NP_055058.1 |
| RefSeq Size | 5836 bp |
| RefSeq ORF | 3618 bp |
| Locus ID | 9508 |
| UniProt ID | O15072 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 135.6 kDa |
| Gene Summary | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease, a member of the procollagen aminopropeptidase subfamily of proteins, may play a role in the processing of type II fibrillar collagen in articular cartilage. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211602L1 | Lenti-ORF clone of ADAMTS3 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 14,984.00 |
|
| RC211602L2 | Lenti-ORF clone of ADAMTS3 (mGFP-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 7,600.00 |
|
| RC211602L3 | Lenti-ORF clone of ADAMTS3 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 7,600.00 |
|
| RC211602L4 | Lenti-ORF clone of ADAMTS3 (mGFP-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 7,600.00 |
|
| RG211602 | ADAMTS3 (tGFP-tagged) - Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 14,184.00 |
|
| SC120706 | ADAMTS3 (untagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 3 (ADAMTS3) |
CNY 12,592.00 |
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