DNAJB7 (NM_145174) Human Tagged ORF Clone

CAT#: RC211335

DNAJB7 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7)

ORF Plasmid: DDK tGFP



  "NM_145174" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DNAJB7 Antibody - C-terminal region
    • 50 ug

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DJ5; HSC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211335 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGATTACTATGAAGTTCTAGGACTGCAAAGATATGCTTCACCTGAGGACATTAAAAAAGCTTATC
ATAAAGTGGCACTTAAATGGCACCCTGATAAAAATCCAGAAAATAAAGAAGAAGCAGAGAGAAAATTCAA
AGAAGTAGCTGAGGCATACGAGGTATTATCAAATGATGAGAAACGGGACATTTATGATAAATATGGCACA
GAAGGATTAAACGGAGGTGGAAGTCATTTTGATGATGAATGTGAGTACGGCTTCACATTCCATAAGCCAG
ATGATGTTTTTAAAGAAATTTTTCATGAAAGGGATCCATTTTCTTTTCACTTCTTTGAAGACTCGCTTGA
GGACCTGTTAAATCGTCCAGGAAGCTCCTATGGAAACAGAAACAGAGATGCAGGATACTTTTTCTCCACT
GCCAGTGAATATCCAATTTTTGAGAAATTTTCTTCATATGATACAGGATATACATCACAGGGTTCATTGG
GGCATGAAGGCCTTACTTCTTTCTCTTCCCTGGCTTTTGATAATAGTGGGATGGACAACTACATATCTGT
TACAACTTCAGACAAAATCGTTAATGGCAGAAATATTAATACAAAGAAAATTATTGAAAGTGATCAAGAA
AGAGAAGCTGAAGATAATGGAGAGTTGACATTTTTTCTTGTAAATAGTGTGGCCAATGAAGAGGGCTTTG
CAAAAGAATGCAGCTGGAGAACACAGTCATTCAACAACTATTCACCAAATTCTCACAGCTCCAAACATGT
ATCTCAATATACTTTCGTGGACAATGATGAGGGAGGTATATCTTGGGTTACCAGCAACAGAGATCCCCCT
ATTTTCTCAGCAGGAGTCAAAGAGGGTGGTAAGAGGAAAAAAAAGAAGCGTAAAGAGGTGCAAAAGAAGT
CTACCAAAAGGAATTGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211335 protein sequence
Red=Cloning site Green=Tags(s)

MVDYYEVLGLQRYASPEDIKKAYHKVALKWHPDKNPENKEEAERKFKEVAEAYEVLSNDEKRDIYDKYGT
EGLNGGGSHFDDECEYGFTFHKPDDVFKEIFHERDPFSFHFFEDSLEDLLNRPGSSYGNRNRDAGYFFST
ASEYPIFEKFSSYDTGYTSQGSLGHEGLTSFSSLAFDNSGMDNYISVTTSDKIVNGRNINTKKIIESDQE
REAEDNGELTFFLVNSVANEEGFAKECSWRTQSFNNYSPNSHSSKHVSQYTFVDNDEGGISWVTSNRDPP
IFSAGVKEGGKRKKKKRKEVQKKSTKRNC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145174
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145174.2
RefSeq Size 2578 bp
RefSeq ORF 930 bp
Locus ID 150353
UniProt ID Q7Z6W7
Protein Families Druggable Genome
MW 35.4 kDa
Gene Summary The protein encoded by this intronless gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain.[provided by RefSeq, Mar 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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