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KLF8 (NM_007250) Human Tagged ORF Clone
CAT＃: RC211273
KLF8 (Myc-DDK-tagged)-Human Kruppel-like factor 8 (KLF8), transcript variant 1
ORF Plasmid: tGFP
"NM_007250" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BKLF3; ZNF741 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211273 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCGATATGGATAAACTCATAAACAACTTGGAGGTCCAACTTAATTCAGAAGGTGGCTCAATGCAGG TATTCAAGCAGGTCACTGCTTCTGTTCGGAACAGAGATCCCCCTGAGATAGAATACAGAAGTAATATGAC TTCTCCAACACTCCTGGATGCCAACCCCATGGAGAACCCAGCACTGTTTAATGACATCAAGATTGAGCCC CCAGAAGAACTTTTGGCTAGTGATTTCAGCCTGCCCCAAGTGGAACCAGTTGACCTCTCCTTTCACAAGC CCAAGGCTCCTCTCCAGCCTGCTAGCATGCTACAAGCTCCAATACGTCCCCCCAAGCCACAGTCTTCTCC CCAGACCCTTGTGGTGTCCACGTCAACATCTGACATGAGCACTTCAGCAAACATTCCTACTGTTCTGACC CCAGGCTCTGTCCTGACCTCCTCTCAGAGCACTGGTAGCCAGCAGATCTTACATGTCATTCACACTATCC CCTCAGTCAGTCTGCCAAATAAGATGGGTGGCCTGAAGACCATCCCAGTGGTAGTGCAGTCTCTGCCCAT GGTGTATACTACTTTGCCTGCAGATGGGGGCCCTGCAGCCATTACAGTCCCACTCATTGGAGGAGATGGT AAAAATGCTGGATCAGTGAAAGTTGACCCCACCTCCATGTCTCCACTGGAAATTCCAAGTGACAGTGAGG AGAGTACAATTGAGAGTGGATCCTCAGCCTTGCAGAGTCTGCAGGGACTACAGCAAGAACCAGCAGCAAT GGCCCAAATGCAGGGAGAAGAGTCGCTTGACTTGAAGAGAAGACGGATTCACCAATGTGACTTTGCAGGA TGCAGCAAAGTGTACACCAAAAGCTCTCACCTGAAAGCTCACCGCAGAATCCATACAGGAGAGAAGCCTT ATAAATGCACCTGGGATGGCTGCTCCTGGAAATTTGCTCGCTCAGATGAGCTCACTCGCCATTTCCGCAA GCACACAGGCATCAAGCCTTTTCGGTGCACAGACTGCAACCGCAGCTTTTCTCGTTCTGACCACCTGTCC CTGCATCGCCGTCGCCATGACACCATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC211273 protein sequence
Red=Cloning site Green=Tags(s) MVDMDKLINNLEVQLNSEGGSMQVFKQVTASVRNRDPPEIEYRSNMTSPTLLDANPMENPALFNDIKIEP PEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQTLVVSTSTSDMSTSANIPTVLT PGSVLTSSQSTGSQQILHVIHTIPSVSLPNKMGGLKTIPVVVQSLPMVYTTLPADGGPAAITVPLIGGDG KNAGSVKVDPTSMSPLEIPSDSEESTIESGSSALQSLQGLQQEPAAMAQMQGEESLDLKRRRIHQCDFAG CSKVYTKSSHLKAHRRIHTGEKPYKCTWDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLS LHRRRHDTM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007250 |
| ORF Size | 1077 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007250.5, NP_009181.2 |
| RefSeq Size | 5434 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 11279 |
| UniProt ID | O95600 |
| Protein Families | Transcription Factors |
| MW | 39.3 kDa |
| Gene Summary | This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211273L1 | Lenti ORF clone of Human Kruppel-like factor 8 (KLF8), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC211273L2 | Lenti ORF clone of Human Kruppel-like factor 8 (KLF8), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC211273L3 | Lenti ORF clone of Human Kruppel-like factor 8 (KLF8), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211273L4 | Lenti ORF clone of Human Kruppel-like factor 8 (KLF8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG211273 | KLF8 (tGFP-tagged) - Human Kruppel-like factor 8 (KLF8), transcript variant 1 |
CNY 4,370.00 |
|
| SC310502 | KLF8 (untagged)-Human Kruppel-like factor 8 (KLF8), transcript variant 1 |
CNY 7,220.00 |
