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GLIS1 (NM_147193) Human Tagged ORF Clone
CAT＃: RC211202
GLIS1 (Myc-DDK-tagged)-Human GLIS family zinc finger 1 (GLIS1)
ORF Plasmid: tGFP
"NM_147193" in other vectors (6)
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CNY 4,616.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211202 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGCCCGCACATCCCTGTCTGCCCACTGTCGGGGCCCGCTGGCCACTGGCCTGCACCCAGACC TGGACCTCCCGGGCCGAAGCCTCGCCACCCCTGCGCCTTCCTGCTACCTTCTGGGCAGCGAACCCAGCTC TGGCCTGGGCCTCCAGCCCGAGACCCACCTCCCCGAGGGCAGCCTGAAGCGGTGCTGCGTCTTGGGCCTA CCCCCCACCTCCCCAGCCTCCTCCTCACCCTGTGCCTCCTCCGACGTCACCTCCATCATCCGCTCCTCCC AGACGTCTCTGGTCACCTGTGTAAATGGACTCCGGAGCCCCCCTCTGACGGGAGATCTGGGGGGCCCTTC CAAGCGGGCCCGGCCTGGCCCTGCATCGACGGACAGCCATGAGGGCAGCTTGCAACTTGAAGCCTGCCGG AAGGCGAGCTTCCTGAAGCAGGAACCCGCGGATGAGTTTTCAGAGCTCTTTGGGCCTCACCAGCAGGGCC TGCCGCCCCCCTATCCCCTGTCTCAGTTGCCGCCTGGCCCAAGCCTTGGAGGCCTGGGGCTGGGCCTGGC AGGCAGGGTGGTGGCCGGGCGGCAGGCGTGCCGCTGGGTGGACTGCTGTGCAGCCTATGAGCAGCAGGAG GAGCTGGTGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAGGACTTCACCTGCTTCTGGG CTGGCTGCGTGCGCCGCTACAAGCCCTTCAACGCCCGCTACAAGCTGCTCATCCACATGCGAGTGCACTC GGGCGAGAAGCCCAACAAGTGCATGTTTGAAGGCTGCAGCAAGGCCTTCTCACGGCTGGAGAACCTCAAG ATCCACCTGAGGAGCCACACGGGCGAGAAGCCGTACCTGTGCCAGCACCCGGGTTGCCAGAAGGCCTTCA GCAACTCCAGCGACCGCGCCAAGCACCAGCGCACCCACCTAGACACGAAGCCGTACGCCTGTCAGATCCC TGGCTGCTCCAAGCGCTACACAGACCCCAGCTCCCTCCGCAAGCACGTCAAGGCCCATTCAGCCAAAGAG CAGCAGGTGCGTAAGAAGCTGCATGCGGGCCCTGACACCGAGGCCGACGTCCTGACCGAGTGTCTGGTCC TGCAGCAGCTCCACACGTCCACACAGCTGGCTGCCAGCGACGGCAAGGGTGGCTGTGGCCTGGGCCAGGA GCTGCTCCCAGGTGTGTATCCTGGCTCCATCACCCCCCATAACGGACTTGCATCGGGCCTCCTGCCCCCA GCGCACGACGTACCTTCCAGGCACCACCCGCTGGATGCCACCACCAGTTCCCACCACCATCTGTCCCCTC TGCCCATGGCTGAGAGCACCCGGGATGGGTTGGGGCCCGGCCTCCTCTCACCAATAGTCAGCCCCCTGAA GGGGCTGGGGCCACCGCCGCTGCCCCCATCCTCTCAGAGCCATTCTCCGGGGGGCCAGCCCTTCCCCACA CTCCCCAGCAAGCCGTCCTACCCACCCTTCCAGAGCCCTCCACCCCCGCCTCTGCCCAGCCCACAAGGTT ACCAGGGCAGTTTCCACTCCATCCAGAGTTGCTTCCCCTATGGCGACTGCTACCGGATGGCTGAACCAGC AGCCGGTGGGGACGGACTGGTCGGGGAGACCCACGGTTTCAACCCCCTGCGGCCCAATGGCTACCACAGC CTCAGCACGCCCTTGCCTGCCACAGGCTATGAGGCCCTGGCTGAGGCCTCATGCCCCACAGCGCTGCCAC AGCAGCCATCTGAAGATGTGGTGTCCAGCGGCCCCGAGGACTGTGGCTTCTTCCCCAATGGAGCCTTTGA CCACTGCCTGGGCCACATCCCCTCCATCTACACAGACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211202 protein sequence
Red=Cloning site Green=Tags(s) MAEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGL PPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACR KASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQE ELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLK IHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKE QQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPP AHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPT LPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHS LSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147193 |
| ORF Size | 1860 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147193.2 |
| RefSeq Size | 2816 bp |
| RefSeq ORF | 1863 bp |
| Locus ID | 148979 |
| UniProt ID | Q8NBF1 |
| MW | 66 kDa |
| Gene Summary | GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211202L1 | Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), Myc-DDK-tagged |
CNY 7,016.00 |
|
| RC211202L2 | Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), mGFP tagged |
CNY 7,016.00 |
|
| RC211202L3 | Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), Myc-DDK-tagged |
CNY 7,016.00 |
|
| RC211202L4 | Lenti ORF clone of Human GLIS family zinc finger 1 (GLIS1), mGFP tagged |
CNY 7,016.00 |
|
| RG211202 | GLIS1 (tGFP-tagged) - Human GLIS family zinc finger 1 (GLIS1) |
CNY 6,216.00 |
|
| SC306307 | GLIS1 (untagged)-Human GLIS family zinc finger 1 (GLIS1) |
CNY 4,624.00 |
