G6PC2 (NM_021176) Human Tagged ORF Clone
CAT#: RC211146
G6PC2 (Myc-DDK-tagged)-Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1
ORF Plasmid: tGFP
"NM_021176" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | IGRP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211146 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCCTTCACAGGAATGGAGTGCTCATAATTCAGCATTTGCAGAAGGACTACCGAGCTTACTACA CTTTTCTAAATTTTATGTCCAATGTTGGAGACCCCAGGAATATCTTTTTCATTTATTTTCCACTTTGTTT TCAATTTAATCAGACAGTTGGAACCAAGATGATATGGGTAGCAGTCATTGGGGATTGGTTAAATCTTATA TTTAAATGGATATTATTTGGTCATCGACCTTACTGGTGGGTCCAAGAAACTCAGATTTACCCAAATCACT CAAGTCCATGCCTTGAACAGTTCCCTACTACATGTGAAACAGGTCCAGGAAGTCCATCTGGCCATGCAAT GGGCGCATCCTGTGTCTGGTATGTCATGGTAACCGCTGCCCTGAGCCACACTGTCTGTGGGATGGATAAG TTCTCTATCACTCTGCACAGACTGACCTGGTCATTTCTTTGGAGTGTTTTTTGGTTGATTCAAATCAGTG TCTGCATCTCCAGAGTATTCATAGCAACACATTTTCCTCATCAAGTTATTCTTGGAGTAATTGGTGGCAT GCTGGTGGCAGAGGCCTTTGAACACACTCCAGGCATCCAAACGGCCAGTCTGGGCACATACCTGAAGACC AACCTCTTTCTCTTCCTGTTTGCAGTTGGCTTTTACCTGCTTCTTAGGGTGCTCAACATTGACCTGCTGT GGTCCGTGCCCATAGCCAAAAAGTGGTGTGCTAACCCCGACTGGATCCACATTGACACCACGCCTTTTGC TGGACTCGTGAGAAACCTTGGGGTCCTCTTTGGCTTGGGCTTTGCAATCAACTCAGAGATGTTCCTCCTG AGCTGCCGAGGGGGAAATAACTACACACTGAGCTTCCGGTTGCTCTGTGCCTTGACCTCATTGACAATAC TGCAGCTCTACCATTTCCTCCAGATCCCGACTCACGAAGAGCATTTATTTTATGTGCTGTCTTTTTGTAA AAGTGCATCCATTCCCCTAACTGTGGTTGCTTTCATTCCCTACTCTGTTCATATGTTAATGAAACAAAGC GGAAAGAAGAGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211146 protein sequence
Red=Cloning site Green=Tags(s) MDFLHRNGVLIIQHLQKDYRAYYTFLNFMSNVGDPRNIFFIYFPLCFQFNQTVGTKMIWVAVIGDWLNLI FKWILFGHRPYWWVQETQIYPNHSSPCLEQFPTTCETGPGSPSGHAMGASCVWYVMVTAALSHTVCGMDK FSITLHRLTWSFLWSVFWLIQISVCISRVFIATHFPHQVILGVIGGMLVAEAFEHTPGIQTASLGTYLKT NLFLFLFAVGFYLLLRVLNIDLLWSVPIAKKWCANPDWIHIDTTPFAGLVRNLGVLFGLGFAINSEMFLL SCRGGNNYTLSFRLLCALTSLTILQLYHFLQIPTHEEHLFYVLSFCKSASIPLTVVAFIPYSVHMLMKQS GKKSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021176 |
ORF Size | 1065 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021176.3 |
RefSeq Size | 3096 bp |
RefSeq ORF | 1068 bp |
Locus ID | 57818 |
UniProt ID | Q9NQR9 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Adipocytokine signaling pathway, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism |
MW | 40.6 kDa |
Gene Summary | This gene encodes an enzyme belonging to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyzes the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, allowing the release of glucose into the bloodstream. The family member encoded by this gene is found in pancreatic islets and does not exhibit phosphohydrolase activity, but it is a major target of cell-mediated autoimmunity in diabetes. Several alternatively spliced transcript variants of this gene have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Tissue-Specific Alteration of Metabolic Pathways Influences Glycemic Regulation
,Ng, NHJ;Willems, SM;Fernandez, J;Fine, RS;Wheeler, E;,
BioRxiv
[G6PC2]
|
Identification and functional characterization of G6PC2 coding variants influencing glycemic traits define an effector transcript at the G6PC2-ABCB11 locus
,Mahajan, A;Sim, X;Ng, HJ;Manning, A;Rivas, MA;Highland, HM;Locke, AE;Grarup, N;Im, HK;Cingolani, P;Flannick, J;Fontanillas, P;Fuchsberger, C;Gaulton, KJ;Teslovich, TM;Rayner, NW;Robertson, NR;Beer, NL;Rundle, JK;Bork-Jensen, J;Ladenvall, C;Blancher, C;Buc,
PLoS Genet.
,PubMed ID 25625282
[G6PC2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211146L1 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC211146L2 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC211146L3 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC211146L4 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG211146 | G6PC2 (tGFP-tagged) - Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1 |
CNY 5,256.00 |
|
SC304916 | G6PC2 (untagged)-Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1 |
CNY 7,220.00 |