NLRX1 (NM_024618) Human Tagged ORF Clone

CAT#: RC211111

NLRX1 (Myc-DDK-tagged)-Human NLR family member X1 (NLRX1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_024618" in other vectors (6)

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CNY 7,144.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NLRX1 mouse monoclonal antibody,clone OTI4H8
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CLR11.3; DLNB26; NOD5; NOD9; NOD26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211111 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTGGGGCCACCATTTGCCCAGGGCCTCTTGGGGCTCTGGTTTTAGAAGAGCACTCCAGCGACCAG
ATGATCGTATCCCCTTCCTGATCCACTGGAGTTGGCCCCTTCAAGGGGAGCGTCCCTTTGGGCCCCCTAG
GGCCTTTATACGCCACCACGGAAGCTCGGTAGATAGCGCTCCCCCACCCGGGAGGCATGGACGGCTGTTC
CCCAGCGCCTCTGCAACTGAAGCTATACAGCGGCACCGCCGGAACCTGGCTGAGTGGTTCAGCCGGCTGC
CCAGGGAGGAGCGCCAGTTTGGCCCAACCTTTGCCCTAGACACGGTCCACGTTGACCCTGTGATCCGCGA
GAGTACCCCTGATGAGCTACTTCGCCCACCCGCGGAGCTGGCCCTGGAGCATCAGCCACCCCAGGCCGGG
CTCCCCCCACTGGCCTTGTCTCAGCTCTTTAACCCGGATGCCTGTGGGCGCCGGGTGCAGACAGTGGTGC
TGTATGGGACAGTGGGCACAGGCAAGAGCACGCTGGTGCGCAAGATGGTTCTGGACTGGTGTTATGGGCG
GCTGCCGGCCTTCGAGCTGCTCATCCCCTTCTCCTGTGAGGACCTGTCATCCCTGGGCCCTGCCCCAGCC
TCCCTGTGCCAACTTGTGGCCCAGCGCTACACGCCCCTGAAGGAGGTTCTGCCCCTGATGGCTGCTGCTG
GGTCCCACCTCCTCTTTGTGCTCCATGGCTTAGAGCATCTCAACCTCGACTTCCGGCTGGCAGGCACGGG
ACTTTGTAGTGACCCGGAGGAACCGCAGGAACCAGCTGCTATCATCGTCAACCTGCTGCGCAAATACATG
CTGCCTCAGGCCAGCATTCTGGTGACCACTCGGCCCTCTGCCATTGGCCGTATCCCCAGCAAGTACGTGG
GCCGCTATGGTGAGATCTGCGGTTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTCAA
CCAGCCGTACTGCGGGTATGCCGTTGGCGGTTCAGGTGTCTCTGCCACACCAGCTCAGCGTGACCACCTG
GTGCAGATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATAGCCGCTGCCTGCTTCCTGCCGTCCTATT
GCTGGCTCGTTTGTGCCACCTTGCACTTCCTGCATGCCCCCACGCCTGCTGGGCAGACCCTTACAAGCAT
CTATACCAGCTTCCTGCGCCTCAACTTCAGCGGGGAAACCCTGGACAGCACTGACCCCTCCAATTTGTCC
CTGATGGCCTATGCAGCCCGAACCATGGGCAAGTTGGCCTATGAGGGGGTGTCCTCCCGCAAGACCTACT
TCTCTGAAGAGGATGTCTGTGGCTGCCTGGAGGCTGGCATCAGGACGGAGGAGGAGTTTCAGCTGCTGCA
CATCTTCCGTCGGGATGCCCTGAGGTTTTTCCTGGCCCCATGTGTGGAGCCAGGGCGTGCAGGCACCTTC
GTGTTCACCGTGCCCGCCATGCAGGAATACCTGGCTGCCCTCTACATTGTGCTGGGTTTGCGCAAGACGA
CCCTGCAAAAGGTGGGCAAGGAAGTGGCTGAGCTCGTGGGCCGTGTTGGGGAGGACGTCAGCCTGGTACT
GGGCATCATGGCCAAGCTGCTGCCTCTGCGGGCTCTGCCTCTGCTCTTCAACCTGATCAAGGTGGTTCCA
CGAGTGTTTGGGCGCATGGTGGGTAAAAGCCGGGAGGCGGTGGCTCAGGCCATGGTGCTGGAGATGTTTC
GAGAGGAGGACTACTACAACGATGATGTTCTGGACCAGATGGGCGCCAGTATCCTGGGCGTGGAGGGCCC
CCGGCGCCACCCAGATGAGCCCCCTGAGGATGAAGTCTTCGAGCTCTTCCCCATGTTCATGGGGGGGCTT
CTCTCTGCCCACAACCGAGCTGTGCTAGCTCAGCTTGGCTGCCCCATCAAGAACCTGGATGCCCTGGAGA
ATGCCCAGGCCATCAAGAAGAAGCTGGGCAAGCTGGGCCGGCAGGTGCTGCCCCCATCAGAGCTCCTTGA
CCACCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCCGCTGAGGTGCTCAGCTCCCTGCGTCAG
CTCAACCTGGCAGGTGTGCGCATGACACCAGTCAAGTGCACAGTGGTGGCAGCTGTGCTGGGCAGCGGAA
GGCATGCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTAGATCCTGCTGGGCTGCGCACACTCCTGCC
TGTCTTCCTGCGTGCCCGGAAGCTGGGCTTGCAACTCAACAGCCTGGGCCCTGAGGCCTGCAAGGACCTC
CGAGACCTGTTGCTGCATGACCAGTGCCAAATTACCACACTGCGGCTGTCCAACAACCCGCTGACGGCGG
CAGGTGTTGCCGTGCTAATGGAGGGGCTGGCAGGAAACACCTCAGTGACGCACCTGTCCCTGCTGCACAC
GGGCCTTGGGGACGAAGGCCTGGAGCTGCTGGCTGCCCAGCTGGACCGCAACCGGCAGCTGCAGGAGCTG
AACGTGGCGTACAACGGTGCTGGTGACACAGCGGCCCTGGCCCTGGCCAGAGCTGCCCGGGAGCACCCTT
CCCTGGAACTGCTACACCTCTACTTCAATGAGCTGAGCTCAGAGGGCCGCCAGGTCTTGCGAGACTTGGG
GGGTGCTGCTGAAGGTGGTGCCCGGGTGGTGGTGTCACTGACAGAGGGGACGGCGGTGTCAGAATACTGG
TCAGTGATCCTCAGTGAAGTCCAGCGGAACCTCAATAGCTGGGATCGGGCCCGGGTTCAGCGACACCTTG
AGCTCCTACTGCGGGATCTGGAAGATAGCCGGGGTGCCACCCTTAATCCTTGGCGCAAGGCCCAGCTGCT
GCGAGTGGAGGGCGAGGTCAGGGCCCTCCTGGAGCAGCTGGGAAGCTCTGGAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211111 protein sequence
Red=Cloning site Green=Tags(s)

MRWGHHLPRASWGSGFRRALQRPDDRIPFLIHWSWPLQGERPFGPPRAFIRHHGSSVDSAPPPGRHGRLF
PSASATEAIQRHRRNLAEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAG
LPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPA
SLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYM
LPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHL
VQMLSRNLEGHHQIAAACFLPSYCWLVCATLHFLHAPTPAGQTLTSIYTSFLRLNFSGETLDSTDPSNLS
LMAYAARTMGKLAYEGVSSRKTYFSEEDVCGCLEAGIRTEEEFQLLHIFRRDALRFFLAPCVEPGRAGTF
VFTVPAMQEYLAALYIVLGLRKTTLQKVGKEVAELVGRVGEDVSLVLGIMAKLLPLRALPLLFNLIKVVP
RVFGRMVGKSREAVAQAMVLEMFREEDYYNDDVLDQMGASILGVEGPRRHPDEPPEDEVFELFPMFMGGL
LSAHNRAVLAQLGCPIKNLDALENAQAIKKKLGKLGRQVLPPSELLDHLFFHYEFQNQRFSAEVLSSLRQ
LNLAGVRMTPVKCTVVAAVLGSGRHALDEVNLASCQLDPAGLRTLLPVFLRARKLGLQLNSLGPEACKDL
RDLLLHDQCQITTLRLSNNPLTAAGVAVLMEGLAGNTSVTHLSLLHTGLGDEGLELLAAQLDRNRQLQEL
NVAYNGAGDTAALALARAAREHPSLELLHLYFNELSSEGRQVLRDLGGAAEGGARVVVSLTEGTAVSEYW
SVILSEVQRNLNSWDRARVQRHLELLLRDLEDSRGATLNPWRKAQLLRVEGEVRALLEQLGSSGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024618
ORF Size 2925 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024618.4
RefSeq Size 3777 bp
RefSeq ORF 2928 bp
Locus ID 79671
UniProt ID Q86UT6
Domains LRR, LRR_RI
Protein Pathways RIG-I-like receptor signaling pathway
MW 107.6 kDa
Gene Summary The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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