MC4 Receptor (MC4R) (NM_005912) Human Tagged ORF Clone
CAT#: RC211026
MC4R (Myc-DDK-tagged)-Human melanocortin 4 receptor (MC4R)
ORF Plasmid: tGFP
"NM_005912" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BMIQ20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211026 representing NM_005912
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTCCACCCACCGTGGGATGCACACTTCTCTGCACCTCTGGAACCGCAGCAGTTACAGACTGC ACAGCAATGCCAGTGAGTCCCTTGGAAAAGGCTACTCTGATGGAGGGTGCTACGAGCAACTTTTTGTCTC TCCTGAGGTGTTTGTGACTCTGGGTGTCATCAGCTTGTTGGAGAATATCTTAGTGATTGTGGCAATAGCC AAGAACAAGAATCTGCATTCACCCATGTACTTTTTCATCTGCAGCTTGGCTGTGGCTGATATGCTGGTGA GCGTTTCAAATGGATCAGAAACCATTGTCATCACCCTATTAAACAGTACAGATACGGATGCACAGAGTTT CACAGTGAATATTGATAATGTCATTGACTCGGTGATCTGTAGCTCCTTGCTTGCATCCATTTGCAGCCTG CTTTCAATTGCAGTGGACAGGTACTTTACTATCTTCTATGCTCTCCAGTACCATAACATTATGACAGTTA AGCGGGTTGGGATCATCATAAGTTGTATCTGGGCAGCTTGCACGGTTTCAGGCATTTTGTTCATCATTTA CTCAGATAGTAGTGCTGTCATCATCTGCCTCATCACCATGTTCTTCACCATGCTGGCTCTCATGGCTTCT CTCTATGTCCACATGTTCCTGATGGCCAGGCTTCACATTAAGAGGATTGCTGTCCTCCCCGGCACTGGTG CCATCCGCCAAGGTGCCAATATGAAGGGAGCGATTACCTTGACCATCCTGATTGGCGTCTTTGTTGTCTG CTGGGCCCCATTCTTCCTCCACTTAATATTCTACATCTCTTGTCCTCAGAATCCATATTGTGTGTGCTTC ATGTCTCACTTTAACTTGTATCTCATACTGATCATGTGTAATTCAATCATCGATCCTCTGATTTATGCAC TCCGGAGTCAAGAACTGAGGAAAACCTTCAAAGAGATCATCTGTTGCTATCCCCTGGGAGGCCTTTGTGA CTTGTCTAGCAGATAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211026 representing NM_005912
Red=Cloning site Green=Tags(s) MVNSTHRGMHTSLHLWNRSSYRLHSNASESLGKGYSDGGCYEQLFVSPEVFVTLGVISLLENILVIVAIA KNKNLHSPMYFFICSLAVADMLVSVSNGSETIVITLLNSTDTDAQSFTVNIDNVIDSVICSSLLASICSL LSIAVDRYFTIFYALQYHNIMTVKRVGIIISCIWAACTVSGILFIIYSDSSAVIICLITMFFTMLALMAS LYVHMFLMARLHIKRIAVLPGTGAIRQGANMKGAITLTILIGVFVVCWAPFFLHLIFYISCPQNPYCVCF MSHFNLYLILIMCNSIIDPLIYALRSQELRKTFKEIICCYPLGGLCDLSSRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005912 |
ORF Size | 996 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005912.3 |
RefSeq Size | 999 bp |
RefSeq ORF | 999 bp |
Locus ID | 4160 |
UniProt ID | P32245 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 36.8 kDa |
Gene Summary | The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity. [provided by RefSeq, Jan 2010] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
---|
Molecular evolution of the proopiomelanocortin system in Barn owl species
,null,
PLoS ONE
,PubMed ID 32369484
[MC4 Receptor]
|
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis
,Montero-Melendez, T;Nagano, A;Chelala, C;Filer, A;Buckley, CD;Perretti, M;,
Nat Commun
,PubMed ID 32029712
[MC4 Receptor]
|
Ligand Bias and Its Association With Pro-resolving Actions of Melanocortin Drugs
,Patruno, S;Garrido-Mesa, J;Romano, M;Perretti, M;Montero-Melendez, T;,
Front Pharmacol
,PubMed ID 30154720
[MC4 Receptor]
|
MC4R-dependent suppression of appetite by bone-derived lipocalin 2
,Mosialou, I;Shikhel, S;Liu, JM;Maurizi, A;Luo, N;He, Z;Huang, Y;Zong, H;Friedman, RA;Barasch, J;Lanzano, P;Deng, L;Leibel, RL;Rubin, M;Nicholas, T;Chung, W;Zeltser, LM;Williams, KW;Pessin, JE;Kousteni, S;,
Nature
,PubMed ID 28273060
[MC4 Receptor]
|
Obesity-Linked Variants of Melanocortin-4 Receptor Are Misfolded in the Endoplasmic Reticulum and Can Be Rescued to the Cell Surface by a Chemical Chaperone
,Susana Granell, Sameer Mohammad, Ramanagouda Ramanagoudr-Bhojappa, and Giulia Baldini,
Mol. Endocrinol., Sep 2010; 24: 1805 - 1821
[MC4 Receptor]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211026L1 | Lenti ORF clone of Human melanocortin 4 receptor (MC4R), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC211026L2 | Lenti ORF clone of Human melanocortin 4 receptor (MC4R), mGFP tagged |
CNY 5,890.00 |
|
RC211026L3 | Lenti ORF clone of Human melanocortin 4 receptor (MC4R), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC211026L4 | Lenti ORF clone of Human melanocortin 4 receptor (MC4R), mGFP tagged |
CNY 5,890.00 |
|
RG211026 | MC4R (tGFP-tagged) - Human melanocortin 4 receptor (MC4R) |
CNY 4,000.00 |
|
SC116445 | MC4R (untagged)-Human melanocortin 4 receptor (MC4R) |
CNY 12,928.00 |
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