ZNF50 (ZSCAN22) (NM_181846) Human Tagged ORF Clone

CAT#: RC210960

ZSCAN22 (Myc-DDK-tagged)-Human zinc finger and SCAN domain containing 22 (ZSCAN22)

ORF Plasmid: DDK tGFP



  "NM_181846" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-ZSCAN22 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HKR2; ZNF50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210960 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATCCCCAAGCACTCCCTGAGCCCAGTGCCGTGGGAAGAGGACAGCTTCCTTCAAGTGAAGGTGG
AGGAGGAAGAGGAAGCCAGCCTCTCCCAGGGCGGAGAATCCAGCCATGACCACATTGCTCACTCTGAGGC
TGCACGCCTGCGCTTCCGGCACTTCCGCTATGAGGAGGCATCTGGTCCACACGAGGCCCTGGCCCACCTC
CGAGCGCTGTGCTGTCAGTGGCTGCAGCCCGAGGCGCACTCCAAGGAGCAGATACTGGAGCTGCTGGTGC
TGGAGCAGTTCCTGGGTGCGCTGCCCCCAGAGATCCAAGCCTGGGTGGGAGCCCAGAGTCCCAAGAGCGG
AGAGGAAGCCGCTGTGCTGGTGGAGGATCTGACTCAGGTGCTGGACAAGAGAGGATGGGATCCAGGAGCC
GAGCCCACAGAGGCAAGCTGCAAACAGAGTGACCTGGGAGAGTCAGAGCCATCAAATGTCACTGAGACCC
TCATGGGAGGTGTTTCCCTTGGACCCGCCTTTGTCAAGGCATGTGAACCTGAGGGCAGCTCAGAGAGGTC
TGGACTATCAGGGGAGATCTGGACAAAGTCTGTCACCCAACAGATCCACTTCAAGAAAACTTCAGGGCCT
TACAAGGATGTCCCCACAGACCAGCGTGGCCGTGAATCTGGTGCCTCGAGGAACAGTTCTAGTGCGTGGC
CAAACCTCACCTCCCAAGAGAAGCCTCCTTCAGAAGACAAATTTGATCTGGTGGATGCTTATGGGACAGA
GCCTCCATACACCTACTCAGGGAAGAGGTCCTCCAAGTGTCGCGAGTGTAGGAAGATGTTCCAGAGTGCT
TCGGCGCTCGAGGCACACCAGAAGACCCATTCTCGGAAGACCCCATATGCCTGCAGCGAGTGTGGGAAAG
CCTTCAGCCGGAGCACTCACCTCGCCCAGCACCAGGTTGTCCACACAGGGGCGAAGCCCCATGAGTGTAA
GGAATGTGGGAAGGCCTTCAGCCGAGTCACCCACCTGACTCAGCACCAAAGGATTCATACTGGAGAGAAA
CCCTACAAATGTGGGGAATGTGGTAAAACCTTCAGCCGCAGCACTCACCTCACCCAGCACCAGCGGGTGC
ACACGGGGGAGCGGCCCTACGAGTGTGACGCGTGTGGGAAAGCCTTCAGCCAGAGCACGCACCTGACTCA
ACACCAGCGCATCCACACCGGGGAGAAGCCCTACAAGTGTGACGCGTGTGGCCGAGCCTTCAGCGACTGC
TCAGCCCTGATCCGACATCTGAGAATCCACTCTGGAGAGAAGCCATATCAGTGTAAGGTTTGTCCGAAGG
CCTTTGCACAGAGCTCCTCCCTCATTGAGCACCAGAGGATCCACACGGGAGAGAAGCCTTATAAGTGCAG
CGACTGTGGGAAGGCCTTCAGTCGTAGCTCAGCCCTGATGGTTCACTTGCGGATCCACATCACGGTGCTG
CAA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC210960 protein sequence
Red=Cloning site Green=Tags(s)

MAIPKHSLSPVPWEEDSFLQVKVEEEEEASLSQGGESSHDHIAHSEAARLRFRHFRYEEASGPHEALAHL
RALCCQWLQPEAHSKEQILELLVLEQFLGALPPEIQAWVGAQSPKSGEEAAVLVEDLTQVLDKRGWDPGA
EPTEASCKQSDLGESEPSNVTETLMGGVSLGPAFVKACEPEGSSERSGLSGEIWTKSVTQQIHFKKTSGP
YKDVPTDQRGRESGASRNSSSAWPNLTSQEKPPSEDKFDLVDAYGTEPPYTYSGKRSSKCRECRKMFQSA
SALEAHQKTHSRKTPYACSECGKAFSRSTHLAQHQVVHTGAKPHECKECGKAFSRVTHLTQHQRIHTGEK
PYKCGECGKTFSRSTHLTQHQRVHTGERPYECDACGKAFSQSTHLTQHQRIHTGEKPYKCDACGRAFSDC
SALIRHLRIHSGEKPYQCKVCPKAFAQSSSLIEHQRIHTGEKPYKCSDCGKAFSRSSALMVHLRIHITVL
Q

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_181846
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_181846.3
RefSeq Size 4643 bp
RefSeq ORF 1476 bp
Locus ID 342945
UniProt ID P10073
Protein Families Druggable Genome
MW 54.6 kDa
Gene Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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