United States
Germany
Japan
United Kingdom
China
TSGA2 (RSPH1) (NM_080860) Human Tagged ORF Clone
CAT＃: RC210926
RSPH1 (Myc-DDK-tagged)-Human radial spoke head 1 homolog (Chlamydomonas) (RSPH1)
ORF Plasmid: tGFP
"NM_080860" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT79; RSP44; RSPH10A; TSA2; TSGA2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210926 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACCTGGGCTCGGAGGAGTTGGAGGAGGAGGGAGAGAATGATATTGGGGAATATGAGGGGGGTC GGAATGAGGCAGGCGAAAGGCACGGACGTGGGAGGGCACGGCTACCCAACGGGGACACCTACGAAGGGAG CTACGAATTCGGTAAAAGACATGGCCAGGGGATCTACAAATTTAAAAATGGTGCTCGATATATCGGAGAA TATGTTAGAAATAAAAAGCACGGTCAAGGCACTTTTATATATCCAGATGGATCCAGATATGAAGGAGAGT GGGCAAATGACCTGCGGCACGGCCATGGCGTATACTACTACATCAATAATGACACCTACACTGGAGAGTG GTTTGCTCATCAAAGGCATGGGCAAGGCACCTATTTATACGCGGAGACGGGCAGTAAGTATGTTGGCACC TGGGTGAACGGACAGCAGGAGGGCACGGCCGAGCTCATTCACCTGAACCACAGGTACCAGGGCAAGTTCT TGAACAAAAATCCTGTTGGCCCTGGAAAGTATGTATTTGATGTTGGGTGTGAACAACATGGTGAATATCG TTTAACAGATATGGAAAGAGGAGAAGAGGAAGAGGAGGAAGAATTAGTAACTGTTGTTCCAAAATGGAAA GCTACCCAAATCACTGAATTGGCCCTGTGGACACCAACTCTCCCCAAAAAGCCGACCTCTACGGATGGAC CTGGCCAAGACGCTCCAGGAGCTGAGAGTGCAGGAGAACCCGGGGAGGAGGCCCAGGCTCTGCTGGAGGG CTTCGAGGGTGAGATGGACATGAGGCCTGGAGATGAAGATGCAGACGTCCTCCGGGAAGAGAGCCGGGAG TATGACCAGGAGGAGTTCCGCTATGACATGGATGAGGGAAACATTAATTCTGAAGAAGAAGAAACTAGAC AGTCAGACCTCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210926 protein sequence
Red=Cloning site Green=Tags(s) MSDLGSEELEEEGENDIGEYEGGRNEAGERHGRGRARLPNGDTYEGSYEFGKRHGQGIYKFKNGARYIGE YVRNKKHGQGTFIYPDGSRYEGEWANDLRHGHGVYYYINNDTYTGEWFAHQRHGQGTYLYAETGSKYVGT WVNGQQEGTAELIHLNHRYQGKFLNKNPVGPGKYVFDVGCEQHGEYRLTDMERGEEEEEEELVTVVPKWK ATQITELALWTPTLPKKPTSTDGPGQDAPGAESAGEPGEEAQALLEGFEGEMDMRPGDEDADVLREESRE YDQEEFRYDMDEGNINSEEEETRQSDLQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080860 |
| ORF Size | 927 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080860.4 |
| RefSeq Size | 1445 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 89765 |
| UniProt ID | Q8WYR4 |
| MW | 35.1 kDa |
| Gene Summary | This gene encodes a male meiotic metaphase chromosome-associated acidic protein. This gene is expressed in tissues with motile cilia or flagella, including the trachea, lungs, airway brushings, and testes. Mutations in this gene result in primary ciliary dyskinesia-24. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210926L3 | Lenti ORF clone of Human radial spoke head 1 homolog (Chlamydomonas) (RSPH1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210926L4 | Lenti ORF clone of Human radial spoke head 1 homolog (Chlamydomonas) (RSPH1), mGFP tagged |
CNY 5,890.00 |
|
| RG210926 | RSPH1 (tGFP-tagged) - Human radial spoke head 1 homolog (Chlamydomonas) (RSPH1) |
CNY 5,200.00 |
|
| SC305866 | RSPH1 (untagged)-Human radial spoke head 1 homolog (Chlamydomonas) (RSPH1) |
CNY 3,600.00 |
