ASPRV1 (NM_152792) Human Tagged ORF Clone

CAT#: RC210908

ASPRV1 (Myc-DDK-tagged)-Human aspartic peptidase, retroviral-like 1 (ASPRV1)

ORF Plasmid: DDK tGFP



  "NM_152792" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-ASPRV1 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ADLI; MUNO; SASP; SASPase; Taps
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210908 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCCCAGGGGCCAGCCTAGGCATCAAAAAGGCTCTGCAGAGTGAACAGGCCACAGCACTGCCTG
CCTCTGCCCCAGCAGTCAGCCAGCCGACCGCGCCTGCTCCCTCCTGCTTGCCCAAGGCCGGGCAAGTCAT
CCCCGCTCTGCTTCGAGAGGCCCCGTTTTCCAGCGTGATTGCGCCGACACTGCTCTGTGGGTTTCTCTTC
TTGGCGTGGGTTGCTGCTGAGGTTCCAGAGGAGAGCAGCAGGATGGCCGGGAGCGGAGCCAGGAGTGAGG
AAGGCCGCCGGCAGCATGCCTTCGTCCCGGAACCTTTTGATGGGGCCAATGTCGTCCCAAACCTCTGGCT
GCACAGCTTTGAAGTCATCAATGACCTCAACCATTGGGACCATATCACCAAGCTAAGGTTCCTGAAAGAG
TCCCTCAGAGGAGAGGCCCTGGGTGTCTACAATAGGCTCAGTCCCCAGGACCAGGGAGACTATGGGACTG
TGAAAGAGGCCCTCCTGAAGGCCTTTGGGGTCCCTGGGGCTGCCCCCAGCCACCTGCCCAAAGAGATCGT
CTTTGCCAACAGCATGGGTAAGGGCTACTATCTCAAGGGGAAGATTGGCAAAGTGCCCGTGAGGTTCCTG
GTGGACTCTGGGGCCCAGGTCTCTGTGGTCCACCCAAACTTGTGGGAGGAGGTCACTGATGGCGATCTGG
ACACCCTGCAGCCCTTTGAGAATGTGGTAAAGGTGGCCAATGGTGCTGAAATGAAGATCCTGGGTGTCTG
GGATACAGCGGTGTCCCTAGGCAAGCTGAAGCTGAAGGCACAGTTCCTAGTGGCCAATGCGAGTGCCGAG
GAAGCCATCATTGGCACTGATGTGCTCCAGGACCACAATGCTATCCTGGACTTTGAGCACCGCACATGCA
CCCTGAAAGGGAAGAAGTTTCGCCTTCTGCCTGTGGGAGGGTCCCTGGAAGATGAGTTTGACCTGGAGCT
CATAGAGGAGGACCCCTCCTCAGAAGAAGGGCGGCAGGAGCTATCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210908 protein sequence
Red=Cloning site Green=Tags(s)

MGSPGASLGIKKALQSEQATALPASAPAVSQPTAPAPSCLPKAGQVIPALLREAPFSSVIAPTLLCGFLF
LAWVAAEVPEESSRMAGSGARSEEGRRQHAFVPEPFDGANVVPNLWLHSFEVINDLNHWDHITKLRFLKE
SLRGEALGVYNRLSPQDQGDYGTVKEALLKAFGVPGAAPSHLPKEIVFANSMGKGYYLKGKIGKVPVRFL
VDSGAQVSVVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVANASAE
EAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLELIEEDPSSEEGRQELSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152792
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152792.1, NP_690005.1
RefSeq Size 2216 bp
RefSeq ORF 780 bp
Locus ID 151516
UniProt ID Q53RT3
Protein Families Druggable Genome, Protease
MW 37 kDa
Gene Summary Filaggrin is a structural protein that is crucial for in the development and maintenance of the skin barrier. This gene encodes a retroviral-like protease involved in profilaggrin-to-filaggrin processing. Expression is found primarily in the epidermis and inner root sheath of hair follicles. [provided by RefSeq, May 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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