HS3ST5 (NM_153612) Human Tagged ORF Clone
CAT#: RC210876
HS3ST5 (Myc-DDK-tagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5)
ORF Plasmid: tGFP
"NM_153612" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3-OST-5; 3OST5; HS3OST5; NBLA04021 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210876 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTATTCAAACAGCAGGCGTGGCTGAGACAGAAGCTCCTGGTGCTGGGAAGCCTTGCCGTTGGGAGTC TCCTGTATCTAGTCGCCAGAGTTGGGAGCTTGGATAGGCTACAACCCATTTGCCCCATTGAAGGTCGACT GGGTGGAGCCCGCACTCAGGCTGAATTCCCACTTCGCGCCCTGCAGTTTAAGCGTGGCCTGCTGCACGAG TTCCGGAAGGGCAACGCTTCCAAGGAGCAGGTTCGCCTCCATGACCTGGTCCAGCAGCTCCCCAAGGCCA TTATCATTGGGGTGAGGAAAGGAGGCACAAGGGCCCTGCTTGAAATGCTGAACCTACATCCGGCAGTAGT CAAAGCCTCTCAAGAAATCCACTTTTTTGATAATGATGAGAATTATGGTAAGGGCATTGAGTGGTATAGG AAAAAGATGCCTTTTTCCTACCCTCAGCAAATCACAATTGAAAAGAGCCCAGCATATTTTATCACAGAGG AGGTTCCAGAAAGGATTTACAAAATGAACTCATCCATCAAGTTGTTGATCATTGTCAGGGAGCCAACCAC AAGAGCTATTTCTGATTATACTCAGGTGCTAGAGGGGAAGGAGAGGAAGAACAAAACTTATTACAAGTTT GAGAAGCTGGCCATAGACCCTAATACATGCGAAGTGAACACAAAATACAAAGCAGTAAGAACCAGCATCT ACACCAAACATCTGGAAAGGTGGTTGAAATACTTTCCAATTGAGCAATTTCATGTCGTCGATGGAGATCG CCTCATCACGGAACCTCTGCCAGAACTTCAGCTCGTGGAGAAGTTCCTAAATCTGCCTCCAAGGATAAGT CAATACAATTTATACTTCAATGCTACCAGAGGGTTTTACTGCTTGCGGTTTAATATTATCTTTAATAAGT GCCTGGCGGGCAGCAAGGGGCGCATTCATCCAGAGGTGGACCCCTCTGTCATTACTAAATTGCGCAAATT CTTTCATCCTTTTAATCAAAAATTTTACCAGATCACTGGGAGGACATTGAACTGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210876 protein sequence
Red=Cloning site Green=Tags(s) MLFKQQAWLRQKLLVLGSLAVGSLLYLVARVGSLDRLQPICPIEGRLGGARTQAEFPLRALQFKRGLLHE FRKGNASKEQVRLHDLVQQLPKAIIIGVRKGGTRALLEMLNLHPAVVKASQEIHFFDNDENYGKGIEWYR KKMPFSYPQQITIEKSPAYFITEEVPERIYKMNSSIKLLIIVREPTTRAISDYTQVLEGKERKNKTYYKF EKLAIDPNTCEVNTKYKAVRTSIYTKHLERWLKYFPIEQFHVVDGDRLITEPLPELQLVEKFLNLPPRIS QYNLYFNATRGFYCLRFNIIFNKCLAGSKGRIHPEVDPSVITKLRKFFHPFNQKFYQITGRTLNWP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153612 |
ORF Size | 1038 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153612.3, NP_705840.2 |
RefSeq Size | 2744 bp |
RefSeq ORF | 1041 bp |
Locus ID | 222537 |
UniProt ID | Q8IZT8 |
Protein Families | Transmembrane |
Protein Pathways | Heparan sulfate biosynthesis |
MW | 40.4 kDa |
Gene Summary | HS3ST5 belongs to a group of heparan sulfate 3-O-sulfotransferases (EC 2.8.2.23) that transfer sulfate from 3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan sulfate and heparin (Mochizuki et al., 2003 [PubMed 12740361]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210876L3 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210876L4 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5), mGFP tagged |
CNY 5,890.00 |
|
RG210876 | HS3ST5 (tGFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5) |
CNY 5,256.00 |
|
SC306625 | HS3ST5 (untagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5) |
CNY 7,220.00 |