United States
Germany
Japan
United Kingdom
China
MAPRE1 (NM_012325) Human Tagged ORF Clone
CAT＃: RC210749
MAPRE1 (Myc-DDK-tagged)-Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1)
ORF Plasmid: tGFP
"NM_012325" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210749 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGAACGTATACTCAACGTCAGTGACCAGTGATAACCTAAGTCGACATGACATGCTGGCCTGGA TCAATGAGTCTCTGCAGTTGAATCTGACAAAGATCGAACAGTTGTGCTCAGGGGCTGCGTATTGTCAGTT TATGGACATGCTGTTCCCTGGCTCCATTGCCTTGAAGAAAGTGAAATTCCAAGCTAAGCTAGAACACGAG TACATCCAGAACTTCAAAATACTACAAGCAGGTTTTAAGAGAATGGGTGTTGACAAAATAATTCCTGTGG ACAAATTAGTAAAAGGAAAGTTTCAGGACAATTTTGAATTCGTTCAGTGGTTCAAGAAGTTTTTCGATGC AAACTATGATGGAAAAGACTATGACCCTGTGGCTGCCAGACAAGGTCAAGAAACTGCAGTGGCTCCTTCC CTTGTTGCTCCAGCTCTGAATAAACCGAAGAAACCTCTCACTTCTAGCAGTGCAGCTCCCCAGAGGCCCA TCTCAACACAGAGAACCGCTGCGGCTCCTAAGGCTGGCCCTGGTGTGGTGCGAAAGAACCCTGGTGTGGG CAACGGAGACGACGAGGCAGCTGAGTTGATGCAGCAGGTCAACGTATTGAAACTTACTGTTGAAGACTTG GAGAAAGAGAGGGATTTCTACTTCGGAAAGCTACGGAACATTGAATTGATTTGCCAGGAGAACGAGGGGG AAAACGACCCTGTATTGCAGAGGATTGTAGACATTCTGTATGCCACAGATGAAGGCTTTGTGATACCTGA TGAAGGGGGCCCACAGGAGGAGCAAGAAGAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210749 protein sequence
Red=Cloning site Green=Tags(s) MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHE YIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKDYDPVAARQGQETAVAPS LVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDL EKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012325 |
| ORF Size | 804 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012325.3 |
| RefSeq Size | 2640 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 22919 |
| UniProt ID | Q15691 |
| Domains | CH, EB1 |
| Protein Families | Druggable Genome |
| MW | 30 kDa |
| Gene Summary | The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210749L1 | Lenti ORF clone of Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210749L2 | Lenti ORF clone of Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1), mGFP tagged |
CNY 6,000.00 |
|
| RC210749L3 | Lenti ORF clone of Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210749L4 | Lenti ORF clone of Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1), mGFP tagged |
CNY 5,890.00 |
|
| RG210749 | MAPRE1 (tGFP-tagged) - Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1) |
CNY 5,200.00 |
|
| SC115455 | MAPRE1 (untagged)-Human microtubule-associated protein, RP/EB family, member 1 (MAPRE1) |
CNY 3,600.00 |
