PRPSAP2 (NM_002767) Human Tagged ORF Clone

CAT#: RC210719

PRPSAP2 (Myc-DDK-tagged)-Human phosphoribosyl pyrophosphate synthetase-associated protein 2 (PRPSAP2)

ORF Plasmid: DDK tGFP



  "NM_002767" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PRPSAP2 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PAP41
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210719 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTGTGTGACGCCACCTGAATTAGAAACCAAGATGAACATAACCAAAGGTGGTCTGGTGTTGTTTT
CAGCAAACTCGAATTCATCATGTATGGAGCTATCAAAGAAAATTGCAGAGCGGCTAGGGGTGGAGATGGG
CAAAGTGCAGGTTTACCAGGAACCTAACAGAGAAACGAGAGTACAAATTCAAGAGTCTGTGAGGGGAAAA
GATGTTTTCATCATCCAAACTGTTTCGAAGGACGTGAACACCACCATCATGGAGCTCCTGATCATGGTGT
ATGCATGTAAGACCTCTTGTGCCAAGAGCATCATTGGCGTGATACCCTACTTTCCTTACAGCAAGCAGTG
CAAGATGAGAAAAAGAGGCTCCATTGTCTCTAAATTGCTGGCTTCCATGATGTGCAAAGCTGGTCTAACT
CATCTTATTACTATGGATTTACACCAGAAGGAAATTCAGGGCTTCTTCAATATTCCTGTTGACAATTTAA
GAGCATCTCCCTTCTTATTACAGTATATTCAAGAAGAGATCCCAGATTACAGGAATGCAGTAATCGTGGC
CAAGTCTCCAGCCTCGGCGAAGAGGGCACAGTCTTTTGCTGAGCGCCTGCGCCTGGGAATTGCAGTGATT
CATGGAGAGGCGCAGGATGCCGAGTCGGACTTGGTGGATGGACGGCATTCCCCACCCATGGTCAGAAGTG
TGGCTGCCATCCACCCCAGCCTGGAGATCCCCATGCTGATTCCTAAAGAAAAGCCCCCAATCACGGTTGT
GGGTGATGTTGGAGGAAGGATTGCCATCATCGTGGATGACATCATTGATGATGTTGACAGCTTTCTTGCT
GCAGCAGAGACCCTGAAGGAAAGAGGTGCATATAAGATCTTTGTGATGGCAACTCATGGCTTGTTGTCTT
CTGACGCCCCCCGGCGGATTGAAGAGTCTGCCATTGATGAGGTGGTGGTCACCAATACAATTCCACATGA
AGTCCAGAAGCTCCAGTGCCCCAAGATTAAAACTGTGGATATCAGCATGATCCTTTCAGAGGCGATCCGT
CGGATCCACAATGGGGAGTCCATGTCCTACCTTTTCAGAAACATAGGCTTAGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210719 protein sequence
Red=Cloning site Green=Tags(s)

MFCVTPPELETKMNITKGGLVLFSANSNSSCMELSKKIAERLGVEMGKVQVYQEPNRETRVQIQESVRGK
DVFIIQTVSKDVNTTIMELLIMVYACKTSCAKSIIGVIPYFPYSKQCKMRKRGSIVSKLLASMMCKAGLT
HLITMDLHQKEIQGFFNIPVDNLRASPFLLQYIQEEIPDYRNAVIVAKSPASAKRAQSFAERLRLGIAVI
HGEAQDAESDLVDGRHSPPMVRSVAAIHPSLEIPMLIPKEKPPITVVGDVGGRIAIIVDDIIDDVDSFLA
AAETLKERGAYKIFVMATHGLLSSDAPRRIEESAIDEVVVTNTIPHEVQKLQCPKIKTVDISMILSEAIR
RIHNGESMSYLFRNIGLDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002767
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002767.4
RefSeq Size 2021 bp
RefSeq ORF 1110 bp
Locus ID 5636
UniProt ID O60256
Domains Pribosyltran
Protein Families Druggable Genome
MW 40.9 kDa
Gene Summary This gene encodes a protein that associates with the enzyme phosphoribosylpyrophosphate synthetase (PRS). PRS catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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