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Phospholipase c eta 1 (PLCH1) (NM_014996) Human Tagged ORF Clone
CAT＃: RC210686
- TrueORF®
PLCH1 (Myc-DDK-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 2
ORF Plasmid: tGFP
"NM_014996" in other vectors (4)
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CNY 16,530.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PLC-L3; PLC eta 1; PLCL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210686 representing NM_014996
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGATGCAGTCCGGGACACAGATGATCAAGCTGAAACGTGGAACCAAAGGGCTTGTCCGCCTCT TTTACCTGGATGAGCACCGGACACGCCTCCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACT TATTGATTCCATTTACAAAGTGACTGAGGGCCGGCAGTCTGAAATATTCCACAGACAAGCTGAGGGGAAC TTCGACCCCAGCTGCTGCTTCACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATCACCTCCA ACCCCGAGGAGGCCCGCACCTGGATCACAGGCCTCAAGTACCTGATGGCTGGCATCAGTGATGAAGACTC CCTTGCCAAAAGGCAGAGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAATGGT GACGGCTTGCTGAATATTGAAGAGATACATCAGCTGATGCATAAACTGAATGTTAATCTGCCCCGAAGAA AAGTCAGACAAATGTTTCAGGAAGCCGACACAGATGAGAATCAGGGAACTTTGACATTTGAAGAGTTCTG TGTTTTTTACAAAATGATGTCTTTGAGACGAGACCTTTATTTGTTACTTTTGAGCTACAGTGACAAGAAA GATCACCTAACTGTGGAAGAACTGGCTCAGTTTTTGAAGGTGGAGCAAAAGATGAATAATGTGACAACGG ACTATTGTCTTGACATCATAAAGAAGTTTGAAGTTTCAGAAGAAAATAAGGTGAAAAATGTTCTTGGCAT AGAAGGCTTCACGAACTTCATGCGTAGTCCTGCCTGTGACATATTTAACCCATTGCACCATGAAGTGTAC CAAGACATGGATCAGCCCCTCTGCAACTACTACATTGCTTCCTCTCACAATACATACCTGACTGGAGACC AGCTCCTTTCTCAGTCCAAAGTGGATATGTATGCACGGGTGCTGCAAGAGGGCTGTCGCTGTGTGGAAGT TGACTGTTGGGATGGCCCAGATGGAGAGCCAGTAGTACATCATGGTTACACTCTCACTTCAAAAATTCTC TTCAGAGATGTTGTGGAGACCATCAACAAGCATGCCTTTGTGAAGAATGAGTTTCCTGTTATATTGTCTA TCGAGAATCACTGCAGTATCCAGCAGCAAAGGAAGATTGCTCAGTACCTGAAAGGAATATTCGGAGACAA ACTGGACCTGTCATCTGTTGATACAGGGGAGTGCAAGCAGCTTCCAAGCCCTCAAAGTTTGAAAGGCAAA ATTCTAGTGAAGGGTAAGAAGTTGCCTTATCACCTTGGGGATGATGCAGAGGAAGGGGAAGTTTCTGATG AGGACAGTGCAGATGAAATTGAAGACGAGTGCAAATTCAAGCTCCATTATAGTAATGGGACCACTGAGCA TCAGGTGGAATCTTTCATAAGGAAAAAACTGGAGTCACTGTTAAAAGAATCTCAAATTCGAGATAAAGAA GATCCTGATAGTTTCACAGTGCGGGCACTACTGAAGGCCACGCATGAAGGCTTAAATGCACACCTGAAGC AGAGTCCAGATGTAAAGGAAAGTGGAAAGAAATCACATGGACGATCCCTCATGACCAACTTTGGAAAACA TAAGAAAACTACAAAATCACGGTCTAAATCTTACAGTACTGATGATGAGGAAGACACACAGCAGAGTACT GGCAAGGAGGGTGGCCAGCTGTACAGATTGGGTCGCCGAAGGAAAACCATGAAGCTCTGCCGAGAACTCT CTGATTTGGTTGTGTACACAAACTCCGTGGCCGCTCAGGACATTGTGGATGACGGAACCACAGGAAATGT GTTATCATTCAGTGAAACAAGAGCACATCAGGTTGTTCAGCAAAAATCAGAGCAGTTCATGATTTATAAT CAAAAGCAACTCACGAGGATTTACCCCTCTGCCTACCGCATTGATTCCAGTAACTTCAACCCTCTCCCCT ACTGGAACGCAGGCTGCCAGCTAGTGGCACTGAATTATCAATCTGAAGGACGAATGATGCAGTTAAACCG AGCCAAATTCAAGGCAAATGGCAATTGTGGCTATGTCCTCAAACCCCAGCAAATGTGCAAAGGTACTTTC AACCCTTTCTCTGGTGACCCTCTTCCTGCCAACCCCAAAAAGCAGCTCATCCTGAAAGTTATCAGTGGAC AGCAACTCCCCAAACCTCCAGACTCCATGTTTGGAGATCGAGGCGAGATCATTGACCCTTTTGTTGAAGT TGAAATTATTGGATTGCCAGTAGATTGTTGTAAAGATCAAACCCGTGTGGTAGATGACAATGGATTTAAC CCTGTGTGGGAAGAAACACTGACATTTACAGTACACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGT GGGATCACGATCCCATTGGACGAGACTTTGTTGGACAAAGAACTGTGACCTTCAGCAGCTTAGTGCCTGG CTACCGGCATGTCTATTTGGAAGGACTGACAGAAGCATCCATATTTGTACACATAACCATCAATGAAATC TATGGAAAGAACAGACAACTCCAGGGTCTGAAGGGACTGTTCAATAAGAATCCTAGGCACAGTTCTTCAG AAAACAATTCCCATTATGTACGGAAGCGATCCATTGGAGATAGAATTCTGCGACGCACAGCTAGCGCCCC AGCCAAAGGCAGGAAAAAGAGCAAAATGGGCTTCCAAGAAATGGTGGAGATAAAGGATTCTGTGTCCGAG GCCACAAGAGATCAAGATGGCGTGCTGAGGAGGACCACACGCAGTTTGCAAGCACGCCCTGTCTCTATGC CTGTTGACAGAAACCTTCTGGGAGCTTTGTCGCTGCCTGTATCTGAAACAGCAAAAGACATTGAAGGAAA AGAAAACTCTCTAGCAGAAGATAAAGATGGCAGAAGAAAAGGGAAAGCAAGTATAAAAGATCCACATTTT CTAAATTTCAACAAAAAGTTATCATCCTCCTCCAGTGCTCTGCTCCACAAAGATACCAGCCAAGGGGACA CCATTGTATCTACTGCCCACATGTCAGTCACAGGAGAACAGCTGGGCATGTCAAGTCCTAGGGGTGGGAG AACCACATCAAATGCCACAAGCAATTGCCAGGAAAACCCCTGTCCCAGCAAGTCTCTCTCCCCAAAGCAG CATTTGGCTCCCGATCCTGTAGTTAACCCCACACAAGATCTGCATGGTGTGAAAATCAAGGAAAAGGGTA ATCCTGAGGACTTTGTGGAAGGGAAAAGCATCTTGTCAGGAAGCGTCCTTTCTCATAGCAACCTAGAAAT TAAGAACCTGGAAGGTAATAGGGGTAAGGGCCGAGCTGCAACATCCTTTTCTTTGTCAGACGTCTCCATG CTCTGTTCTGACATACCTGACCTACATTCAACTGCAATTCTGCAGGAGAGTGTAATTTCCCATCTTATTG ACAATGTCACTTTAACAAATGAGAATGAGCCGGGCAGTTCCATCTCAGCCCTGATTGGCCAGTTTGATGA GACCAACAATCAGGCTCTCACAGTTGTTTCTCATCTTCATAATACCAGTGTGATGTCAGGCCATTGTCCC TTGCCTAGCCTGGGCCTAAAAATGCCCATCAAGCATGGTTTTTGCAAGGGAAAATCCAAGTCTTCCTTCC TGTGCTCATCTCCGGAGCTGATAGCACTCTCGAGTTCTGAGACCACCAAACATGCAACGAACACAGTTTA TGAAACTACCTGCACTCCCATCTCTAAAACCAAACCAGATGATGACCTTTCTAGTAAGGCCAAGACAGCG GCCTTAGAAAGCAACCTGCCTGGATCCCCTAATACTTCTCGTGGCTGGTTACCAAAAAGTCCTACCAAGG GAGAAGACTGGGAAACACTGAAGAGCTGCAGCCCTGCCTCTTCCCCTGATTTGACCCTGGAGGATGTAAT AGCTGATCCCACTCTCTGTTTCAATTCTGGGGAGAGCAGCCTTGTGGAAATTGATGGAGAATCAGAAAAT CTTTCTCTAACAACCTGTGAATATAGGAGAGAGGGCACAAGTCAACTTGCTTCTCCTTTAAAACTCAAGT ACAATCAGGGTGTGGTAGAACACTTTCAAAGAGGTTTGAGAAACGGCTACTGTAAAGAGACCCTCCGCCC TTCTGTCCCTGAAATATTCAACAATATTCAAGATGTCAAAACTCAAAGTATTTCTTATCTAGCCTATCAG GGTGCTGGCTTTGTGCATAATCATTTCTCAGATTCAGATGCAAAAATGTTCCAGACCTGTGTGCCCCAGC AATCTAGTGCTCAAGATATGCATGTCCCTGTACCCAAGCAGTTGGCACATCTTCCTTTGCCTGCTCTGAA ACTGCCTAGTCCTTGCAAATCCAAAAGTCTGGGGGACTTAACATCAGAGGACATTGCCTGCAATTTTGAG AGCAAGTACCAGTGTATTAGTAAGAGTTTTGTTACAACTGGCATTAGAGACAAGAAGGGCGTGACTGTGA AGACAAAGTCGTTAGAGCCTATAGATGCCCTGACCGAGCAGCTTCGGAAGCTTGTGTCCTTTGACCAGGA AGACAACTGCCAAGTGCTATATTCAAAGCAGGATGCCAATCAGCTCCCCCGGGCACTGGTCAGGAAGTTG TCATCCAGAAGTCAGAGCAGAGTGCGCAATATTGCTAGTCGTGCCAAGGAGAAACAGGAAGCCAACAAGC AGAAAGTTCCAAACCCCAGCAATGGGGCAGGAGTGGTTCTTAGAAACAAACCCTCAGCACCCACCCCTGC AGTGAATCGCCACTCCACCGGCTCCTACATCGCAGGCTACCTGAAGAACACGAAAGGGGGTGGCCTTGAA GGCCGGGGCATCCCAGAGGGGGCATGCACGGCTCTTCACTATGGCCACGTTGACCAGTTTTGTTCAGATA ATTCTGTTTTGCAGACTGAGCCAAGCAGTGATGATAAACCAGAAATTTATTTTCTTTTGAGACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210686 representing NM_014996
Red=Cloning site Green=Tags(s) MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGN FDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNG DGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKK DHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVY QDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKIL FRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGK ILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKE DPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQST GKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYN QKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTF NPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFN PVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEI YGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSE ATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHF LNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQ HLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSM LCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCP LPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTA ALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESEN LSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQ GAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFE SKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKL SSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLE GRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014996 |
| ORF Size | 4965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014996.2, NP_055811.1 |
| RefSeq Size | 6128 bp |
| RefSeq ORF | 5058 bp |
| Locus ID | 23007 |
| UniProt ID | Q4KWH8 |
| Protein Families | Druggable Genome |
| MW | 184.9 kDa |
| Gene Summary | PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210686L3 | Lenti-ORF clone of PLCH1 (Myc-DDK-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 2 |
CNY 18,430.00 |
|
| RC210686L4 | Lenti-ORF clone of PLCH1 (mGFP-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 2 |
CNY 18,430.00 |
|
| RG210686 | PLCH1 (tGFP-tagged) - Human phospholipase C, eta 1 (PLCH1), transcript variant 2 |
CNY 18,150.00 |
|
| SC304200 | PLCH1 (untagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 2 |
CNY 10,450.00 |
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