PLSCR3 (NM_020360) Human Tagged ORF Clone
CAT#: RC210587
PLSCR3 (Myc-DDK-tagged)-Human phospholipid scramblase 3 (PLSCR3), transcript variant 1
ORF Plasmid: tGFP
"NM_020360" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210587 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCTACTTGCCCCCCAAAGGCTACGCCCCTTCGCCCCCACCTCCCTACCCTGTCACCCCTGGGT ACCCGGAGCCGGCGCTACATCCTGGGCCCGGGCAGGCGCCAGTGCCCGCCCAGGTACCTGCCCCAGCTCC CGGCTTCGCCCTCTTCCCCTCGCCTGGCCCCGTGGCCTTGGGGTCTGCTGCCCCCTTCTTGCCACTGCCA GGGGTGCCTTCTGGCCTCGAATTCCTGGTGCAGATTGATCAGATTTTGATTCACCAGAAGGCTGAGCGAG TGGAAACGTTCCTAGGCTGGGAGACCTGTAATCGGTATGAACTGCGCTCTGGGGCCGGGCAGCCCCTGGG TCAGGCGGCCGAGGAGAGCAACTGCTGCGCCCGTCTGTGCTGTGGCGCCCGCCGGCCGCTGCGTGTCCGC CTGGCCGACCCCGGGGACCGTGAGGTGCTGCGTTTGCTCCGCCCGCTGCACTGTGGCTGCAGCTGCTGCC CCTGTGGCCTCCAGGAGATGGAAGTACAGGCTCCACCAGGCACCACCATTGGCCACGTGCTACAGACCTG GCATCCCTTCCTCCCCAAGTTCTCCATCCAGGATGCCGATCGCCAGACAGTCTTGCGAGTGGTGGGGCCC TGCTGGACCTGTGGCTGTGGCACAGACACCAACTTTGAGGTGAAGACTCGGGATGAATCCCGCAGTGTGG GCCGCATCAGCAAGCAGTGGGGGGGCCTGGTCCGAGAAGCCCTCACAGATGCAGATGACTTTGGCCTACA GTTCCCGCTGGACCTGGATGTGAGGGTGAAGGCTGTGCTGCTGGGAGCCACATTCCTCATTGACTACATG TTCTTTGAGAAGCGAGGAGGCGCTGGGCCCTCTGCCGTCACCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210587 protein sequence
Red=Cloning site Green=Tags(s) MAGYLPPKGYAPSPPPPYPVTPGYPEPALHPGPGQAPVPAQVPAPAPGFALFPSPGPVALGSAAPFLPLP GVPSGLEFLVQIDQILIHQKAERVETFLGWETCNRYELRSGAGQPLGQAAEESNCCARLCCGARRPLRVR LADPGDREVLRLLRPLHCGCSCCPCGLQEMEVQAPPGTTIGHVLQTWHPFLPKFSIQDADRQTVLRVVGP CWTCGCGTDTNFEVKTRDESRSVGRISKQWGGLVREALTDADDFGLQFPLDLDVRVKAVLLGATFLIDYM FFEKRGGAGPSAVTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020360 |
ORF Size | 885 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020360.4 |
RefSeq Size | 2089 bp |
RefSeq ORF | 888 bp |
Locus ID | 57048 |
UniProt ID | Q9NRY6 |
Domains | Scramblase |
MW | 31.6 kDa |
Gene Summary | May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Proteomic Profiling of S-acylated Macrophage Proteins Identifies a Role for Palmitoylation in Mitochondrial Targeting of Phospholipid Scramblase 3
,B. Alex Merrick, Suraj Dhungana, Jason G. Williams, Jim J. Aloor, Shyamal Peddada, Kenneth B. Tomer, and Michael B. Fessler,
Mol. Cell. Proteomics, Oct 2011; 10: M110.006007.
[PLSCR3]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210587L3 | Lenti ORF clone of Human phospholipid scramblase 3 (PLSCR3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210587L4 | Lenti ORF clone of Human phospholipid scramblase 3 (PLSCR3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG210587 | PLSCR3 (tGFP-tagged) - Human phospholipid scramblase 3 (PLSCR3), transcript variant 1 |
CNY 4,000.00 |
|
SC113138 | PLSCR3 (untagged)-Human phospholipid scramblase 3 (PLSCR3), transcript variant 1 |
CNY 2,400.00 |