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NIPP1 (PPP1R8) (NM_138558) Human Tagged ORF Clone
CAT＃: RC210575
PPP1R8 (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2
ORF Plasmid: tGFP
"NM_138558" in other vectors (4)
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CNY 3,656.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARD-1; ARD1; NIPP-1; NIPP1; PRO2047 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210575 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACTGCCCAACCTGGGCAGGTAAGCCCC CTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATTATTGATGAGAAGAA GTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATTGACCACCAGTCTTGCTCTCGGGTC CATGCTGCACTTGTCTACCACAAGCATCTGAAGAGAGTTTTCCTGATAGATCTCAACAGTACACACGGCA CTTTCTTGGGTCACATTCGGTTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATT TGGCGCATCCACAAGGGCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGAT GAGAAGATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAAACTGAGCTTG ATAACCTGACAGAGTTCATCACTGCCCACAACAAGCGGATTTCTACCCTTACCATTGAGGAGGGAAATCT GGACATTCAAAGACCAAAGAGGAAGAGGAAGAACTCACGGGTGACATTCAGTGAGGATGATGAGATCATC AACCCAGAGGATGTGGATCCCTCAGTTGGTCGATTCAGGAACATGGTGCAAACTGCAGTGGTCCCAGTCA AGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTT TGCCTTCAGCGGAGGACTCTACGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGC ATCCATGGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGATGTGGACTTGA CTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTATAACCCTGAAGCTGT AAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAGCCCACACCTTCCTTGCTG ATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210575 protein sequence
Red=Cloning site Green=Tags(s) MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRV HAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGD EKMGGEDDELKGLLGLPEEETELDNLTEFITAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEII NPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHG IHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLL I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138558 |
| ORF Size | 1056 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 2659 bp |
| RefSeq ORF | 630 bp |
| Locus ID | 5511 |
| UniProt ID | Q12972 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 38.5 kDa |
| Gene Summary | This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Memory Decline and Its Reversal in Aging and Neurodegeneration Involve miR-183/96/182 Biogenesis
,Jawaid, A;Woldemichael, BT;Kremer, EA;Laferriere, F;Gaur, N;Afroz, T;Polymenidou, M;Mansuy, IM;,
Mol. Neurobiol.
,PubMed ID 30128653
[NIPP1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210575L3 | Lenti-ORF clone of PPP1R8 (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2 |
CNY 5,890.00 |
|
| RC210575L4 | Lenti-ORF clone of PPP1R8 (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2 |
CNY 5,890.00 |
|
| RG210575 | PPP1R8 (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2 |
CNY 5,256.00 |
|
| SC109604 | PPP1R8 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2 |
CNY 2,400.00 |
