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KLF10 (NM_005655) Human Tagged ORF Clone
CAT＃: RC210565
KLF10 (Myc-DDK-tagged)-Human Kruppel-like factor 10 (KLF10), transcript variant 1
ORF Plasmid: tGFP
"NM_005655" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EGR-alpha; EGRA; TIEG; TIEG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210565 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCTGAAAGGCCAA AAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAAGCTGTAGAAGCACTTATGTC AATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTTGAAAACAGACCTGTTACACCAGTATCTGAT TTGTCAGAGGAAGAGAATCTGCTTCCGGGAACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTC CACCTTACAGTCCTTCTGACTTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGT ACACTTCAAGTCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCTGATGCCCAGC TATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTATCAGAACAATTCTTTTAGAAG AAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATACCATGTGCCGCTGTGTCACCAAACAGATCC AAATGTGAGAGAAACACAGTGGCAGATGTTGATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGC CTTCCTCAGAGACGGTCATCTGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGT CTCTCCACCTGCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCCGTTTGCCCCC CTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTGGTACCCCAGCCCGTTGTGCA GAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGACTCTCTCCCATTGCCCCTGCTCCTGGGTTT TCCCCTTCAGCAGCAAAAGTCACTCCTCAGATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACC CAGGATGTGGCAAGACATACTTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAA GCCTTTCAGCTGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGGAGTGACCATT TGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGGCAGATGGAAGTGAGCAAGCT AAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210565 protein sequence
Red=Cloning site Green=Tags(s) MLNFGASLQQTAEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSD LSEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIAAPFKEEEKS PVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHLNVEAARKNIPCAAVSPNRS KCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQQKSVLVSPPAVSAGGVPPMPVICQMVPLP ANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGF SPSAAKVTPQIDSSRIRSHICSHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHR RTHTGEKKFACPMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005655 |
| ORF Size | 1440 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005655.4 |
| RefSeq Size | 3115 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 7071 |
| UniProt ID | Q13118 |
| Domains | zf-C2H2 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 52.6 kDa |
| Gene Summary | This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210565L1 | Lenti ORF clone of Human Kruppel-like factor 10 (KLF10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210565L2 | Lenti ORF clone of Human Kruppel-like factor 10 (KLF10), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC210565L3 | Lenti ORF clone of Human Kruppel-like factor 10 (KLF10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210565L4 | Lenti ORF clone of Human Kruppel-like factor 10 (KLF10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210565 | KLF10 (tGFP-tagged) - Human Kruppel-like factor 10 (KLF10), transcript variant 1 |
CNY 7,088.00 |
|
| SC116618 | KLF10 (untagged)-Human Kruppel-like factor 10 (KLF10), transcript variant 1 |
CNY 5,488.00 |
