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VCIP 135 (VCPIP1) (NM_025054) Human Tagged ORF Clone
CAT＃: RC210546
VCPIP1 (Myc-DDK-tagged)-Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1)
ORF Plasmid: tGFP
"NM_025054" in other vectors (6)
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CNY 8,512.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DUBA3; VCIP135 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210546 representing NM_025054
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAGCCGCCGCCGCCGCCGCCTCCGTTGCCGCCGCCACCTCCTCCCCCTGAGGCTCCACAGACTC CGTCGTCCTTGGCGTCGGCGGCTGCTTCGGGGGGGCTTTTGAAGCGGAGAGACCGGAGAATCCTTTCCGG GAGCTGCCCGGATCCGAAGTGTCAGGCGCGTCTATTTTTCCCGGCCTCCGGTTCTGTCAGCATCGAGTGT ACCGAGTGCGGCCAGCGGCACGAGCAGCAACAGCTGCTGGGGGTTGAGGAGGTGACCGACCCGGACGTAG TGCTACACAACCTGCTGCGGAACGCGCTGCTCGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGT AAAGGTGATGGGCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTATGGAATGGAC AAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCAGGGCGAACTGTTCGATTGCGCCTTACTGG GTGACCGCGCCTTCCTCATAGAACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAG CCTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAGCCAGGAATGTCTCATTCCA GTGCATGTGGACGGGGATGGACACTGCTTGGTGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCT TCTGGCATGCCTTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCAAGCTCTGTT CCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCAATGAGTGTGACCCTCTGTTTGTACCACCT GAGGGTGTTCCCTTGGGCCTGAGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTA TTCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCACCTTTCTACCTGGGCTCAT CCCTGCAGAGAAGTGCACTGGGAAAGATGGTCATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCC GGTAGAAACCATTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTATGAATTTGC TTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAGTACATAAAACTTGAAGAGGATGGTGGTTG TGTTATTGGAGGTGACAGAAGTTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTC TTTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAGTATTTCTACAGAAGGACTG GAGTGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCA CAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGGAGGGAAA TTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTACAGCTTTCCCTTGAACA ATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACCAGACTATGGAATGAGTAACCTAACAGC TTGTAATTGGTGCCATGGCACATCTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGA GACAGAACTAATTCTAGGTCCACTGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGG AGTATGACAATCTACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAGAAACAGT ATATTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATGTTGCAATGAAACTTGTT ACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTCAGAAAGTTGTCCACACTATATTGCATC AGACTGCCAAAAAGAATCCCGATGATTATACTCCTGTAAATATAGATGGTGCTCACGCCCAAAGAGTTGG AGATGTTCAAGGACAAGAATCAGAGTCTCAGCTCCCAACTAAAATTATTCTTACTGGACAGAAAACAAAA ACTTTGCACAAGGAGGAGTTAAACATGAGTAAAACTGAAAGAACTATTCAACAGAATATTACGGAACAGG CTTCTGTAATGCAGAAACGGAAAACAGAGAAGTTAAAACAAGAACAAAAAGGGCAACCCAGGACTGTTTC TCCCAGTACCATTCGTGATGGTCCATCCTCTGCACCTGCTACACCTACCAAGGCTCCCTATTCACCGACA ACTTCTAAGGAGAAGAAGATCCGAATCACAACTAATGATGGACGACAGTCCATGGTTACCCTTAAGTCTT CAACAACCTTTTTTGAACTTCAGGAAAGTATAGCCAGAGAATTCAACATTCCTCCATATTTACAGTGTAT TCGATACGGGTTTCCTCCTAAAGAGTTAATGCCACCACAGGCAGGAATGGAAAAGGAACCAGTTCCTTTA CAGCATGGCGACAGAATTACAATAGAAATTCTAAAAAGTAAAGCTGAAGGTGGTCAGTCTGCTGCAGCAC ACTCAGCCCACACTGTGAAACAAGAAGATATTGCTGTTACTGGTAAACTGTCATCTAAGGAACTTCAGGA GCAAGCTGAAAAAGAAATGTACTCCTTGTGTCTTTTAGCAACATTAATGGGAGAAGATGTATGGTCTTAT GCAAAGGGACTTCCTCACATGTTTCAGCAGGGTGGTGTATTCTACAGTATTATGAAGAAAACCATGGGTA TGGCTGATGGCAAGCATTGTACTTTTCCACATCTGCCTGGCAAAACCTTTGTCTATAATGCTTCTGAAGA TAGACTGGAATTGTGTGTGGATGCTGCAGGACATTTCCCCATTGGTCCTGATGTTGAAGATTTAGTTAAA GAGGCTGTAAGTCAGGTTCGAGCAGAGGCTACTACAAGAAGTAGGGAATCAAGTCCCTCACATGGGCTAT TAAAACTAGGTAGTGGTGGAGTAGTGAAAAAGAAATCTGAGCAACTTCATAACGTAACTGCCTTTCAGGG AAAAGGGCATTCTTTAGGAACTGCATCTGGTAACCCACACCTTGATCCAAGAGCTAGGGAAACTTCAGTT GTAAGAAAGCATAATACAGGGACAGACTTTAGTAATAGTTCCACTAAAACAGAGCCTTCTGTATTCACAG CTTCTTCTAGTAATAGTGAGCTTATTCGAATAGCTCCTGGAGTAGTAACAATGAGAGACGGCAGGCAGCT TGATCCTGATTTGGTTGAGGCCCAGCGAAAAAAATTGCAGGAAATGGTTTCTTCTATTCAGGCTTCAATG GACAGGCACCTTCGGGATCAAAGTACAGAGCAGTCACCATCTGATCTTCCTCAAAGGAAAACAGAAGTTG TGAGTTCTTCTGCAAAGTCTGGGAGTCTTCAGACTGGTTTGCCTGAATCTTTTCCTTTAACTGGTGGTAC TGAAAATTTGAATACAGAAACAACTGATGGCTGTGTAGCAGATGCACTGGGAGCAGCCTTTGCCACAAGG TCAAAAGCACAAAGGGGAAATTCCGTGGAGGAGCTTGAAGAGATGGATAGTCAAGATGCTGAGATGACTA ACACAACTGAGCCAATGGATCACTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210546 representing NM_025054
Red=Cloning site Green=Tags(s) MSQPPPPPPPLPPPPPPPEAPQTPSSLASAAASGGLLKRRDRRILSGSCPDPKCQARLFFPASGSVSIEC TECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMD KQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIP VHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPP EGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSS GRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEV FMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGGK LYNLAKSTHGQLRTDKNYSFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDG DRTNSRSTGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSNVYDVAMKLV TKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQESESQLPTKIILTGQKTK TLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPT TSKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPL QHGDRITIEILKSKAEGGQSAAAHSAHTVKQEDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSY AKGLPHMFQQGGVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVK EAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASGNPHLDPRARETSV VRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASM DRHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATR SKAQRGNSVEELEEMDSQDAEMTNTTEPMDHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025054 |
| ORF Size | 3666 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025054.5 |
| RefSeq Size | 8192 bp |
| RefSeq ORF | 3669 bp |
| Locus ID | 80124 |
| UniProt ID | Q96JH7 |
| MW | 134.1 kDa |
| Gene Summary | Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210546L1 | Lenti ORF clone of Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1), Myc-DDK-tagged |
CNY 10,912.00 |
|
| RC210546L2 | Lenti ORF clone of Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1), mGFP tagged |
CNY 14,060.00 |
|
| RC210546L3 | Lenti ORF clone of Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1), Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC210546L4 | Lenti ORF clone of Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1), mGFP tagged |
CNY 10,912.00 |
|
| RG210546 | VCPIP1 (tGFP-tagged) - Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1) |
CNY 13,400.00 |
|
| SC111292 | VCPIP1 (untagged)-Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1) |
CNY 10,450.00 |
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