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MIDN (NM_177401) Human Tagged ORF Clone
CAT＃: RC210528
- TrueORF®
MIDN (Myc-DDK-tagged)-Human midnolin (MIDN)
ORF Plasmid: tGFP
"NM_177401" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210528 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCAGCCCGGCGGCGCCCGGAGCTGCCGGCGCGGGGCCCCCGGCGGCGCCTGCGAGCTGGGCC CGGCGGCCGAGGCGGCGCCCATGAGCCTCGCCATCCACAGCACCACGGGCACCCGCTACGACCTGGCCGT GCCGCCCGACGAGACGGTGGAGGGGCTGCGCAAGCGGTTGTCCCAGCGCCTCAAAGTGCCCAAGGAGCGC CTGGCTCTTCTCCACAAAGACACCCGGCTCAGTTCGGGGAAGCTGCAGGAGTTCGGCGTGGGTGATGGCA GCAAGCTGACCTTGGTACCCACCGTGGAAGCGGGCCTCATGTCTCAGGCCTCAAGGCCGGAACAGTCCGT GATGCAAGCTCTCGAGAGTCTCACGGAGACGCAGGTCAGTGACTTCCTGTCGGGCCGTTCGCCACTGACA CTGGCCTTGCGTGTGGGCGACCACATGATGTTCGTGCAGCTGCAGCTCGCGGCCCAGCACGCTCCACTGC AACACCGCCATGTGCTGGCCGCTGCGGCCGCCGCCGCTGCTGCGCGGGGGGACCCCAGCATAGCCTCCCC CGTGTCCTCGCCCTGCCGGCCGGTGTCCAGTGCCGCCCGAGTCCCCCCGGTGCCCACCAGCCCGTCCCCT GCATCTCCCTCGCCCATCACAGCCGGCTCCTTCCGGTCCCACGCAGCCTCCACCACCTGCCCGGAGCAGA TGGACTGCTCCCCCACGGCCAGCAGCAGTGCCAGTCCTGGTGCCAGCACCACGTCTACCCCAGGGGCCAG CCCTGCCCCCCGCTCCCGAAAACCCGGCGCCGTCATCGAGAGCTTTGTGAATCACGCCCCGGGGGTCTTC TCAGGGACCTTCTCTGGCACGCTACACCCCAACTGCCAAGACAGCAGCGGGCGGCCGCGGCGTGACATCG GCACCATCCTGCAGATCCTGAACGACCTCCTGAGCGCCACCCGGCACTACCAGGGCATGCCCCCTTCGCT GGCCCAGCTCCGCTGCCACGCCCAGTGCTCCCCGGCCTCACCGGCCCCCGACCTGGCCCCCAGAACTACC TCCTGCGAGAAGCTCACGGCTGCCCCCTCAGCCTCCCTGCTGCAGGGCCAGAGCCAGATCCGCATGTGCA AGCCCCCGGGGGATCGGCTTCGGCAGACAGAAAACCGCGCCACGCGCTGCAAGGTGGAACGGCTGCAGCT GCTTCTGCAGCAGAAACGGCTCCGTAGAAAGGCCCGGCGGGACGCGCGGGGTCCGTACCACTGGTCACCC AGCCGCAAGGCCGGCCGCAGCGACAGCAGTAGCAGCGGGGGCGGCGGCAGCCCCAGCGAGGCCTCCGGCT TGGGCCTCGACTTCGAGGACTCCGTGTGGAAGCCAGAAGCCAACCCTGACATCAAGTCAGAGTTCGTGGT GGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210528 protein sequence
Red=Cloning site Green=Tags(s) MEPQPGGARSCRRGAPGGACELGPAAEAAPMSLAIHSTTGTRYDLAVPPDETVEGLRKRLSQRLKVPKER LALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLT LALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAAAAARGDPSIASPVSSPCRPVSSAARVPPVPTSPSP ASPSPITAGSFRSHAASTTCPEQMDCSPTASSSASPGASTTSTPGASPAPRSRKPGAVIESFVNHAPGVF SGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTT SCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWSP SRKAGRSDSSSSGGGGSPSEASGLGLDFEDSVWKPEANPDIKSEFVVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177401 |
| ORF Size | 1404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177401.4, NP_796375.3 |
| RefSeq Size | 3812 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 90007 |
| UniProt ID | Q504T8 |
| Protein Families | Druggable Genome |
| MW | 49.2 kDa |
| Gene Summary | Facilitates ubiquitin-independent proteasomal degradation of polycomb protein CBX4. Plays a role in inhibiting the activity of glucokinase GCK and both glucose-induced and basal insulin secretion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210528L3 | Lenti-ORF clone of MIDN (Myc-DDK-tagged)-Human midnolin (MIDN) |
CNY 5,890.00 |
|
| RC210528L4 | Lenti-ORF clone of MIDN (mGFP-tagged)-Human midnolin (MIDN) |
CNY 5,890.00 |
|
| RG210528 | MIDN (tGFP-tagged) - Human midnolin (MIDN) |
CNY 4,370.00 |
|
| SC307089 | MIDN (untagged)-Human midnolin (MIDN) |
CNY 3,656.00 |
