United States
Germany
Japan
United Kingdom
China
KCC4 (SLC12A7) (NM_006598) Human Tagged ORF Clone
CAT＃: RC210512
SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7)
ORF Plasmid: tGFP
"NM_006598" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,544.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KCC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210512 representing NM_006598
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGCCCACCAACTTCACCGTGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGCCGAGC GGACGGAGGCTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGGAGATGGAAATCCAAG AGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAGCTTCTTTGAAGGGAAGAACATGGCA CTTTTCGAGGAGGAGATGGACAGTAACCCCATGGTGTCCTCGCTGCTCAACAAGCTGGCCAACTACACCA ACCTGAGCCAGGGCGTGGTGGAGCACGAGGAGGACGAGGAGAGCCGGCGGCGGGAGGCCAAGGCTCCGCG CATGGGCACCTTCATCGGCGTCTACCTGCCGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGC CTGACGTGGATCGTGGGGGTGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACATGTA CAATGCTGACCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGCTGGCGGGTCCTACTA CATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGGCCTCTGCTTCTACCTGGGCACGACG TTTGCAGGGGCCATGTATATTTTGGGGACCATCGAGATTTTTCTGACGTACATCTCCCCGGGTGCGGCCA TCTTCCAGGCGGAGGCTGCAGGTGGCGAGGCGGCCGCCATGCTGCACAACATGCGTGTGTACGGCACGTG CACGCTCGTGCTCATGGCCCTGGTGGTCTTCGTGGGCGTCAAGTATGTCAACAAGCTGGCGCTGGTCTTC CTGGCCTGCGTCGTGCTGTCCATCCTGGCCATCTATGCCGGCGTCATCAAGTCTGCCTTCGACCCCCCGG ACATCCCGGTCTGCCTCCTGGGGAACCGCACGCTGTCACGGCGCAGCTTCGATGCCTGCGTCAAGGCCTA CGGCATCCACAACAACTCAGCCACCTCCGCGCTCTGGGGCCTCTTCTGCAACGGCTCCCAGCCCAGCGCC GCCTGTGACGAGTACTTCATCCAGAACAACGTCACCGAAATCCAGGGCATCCCGGGCGCGGCCAGTGGTG TCTTCCTGGAGAACCTGTGGAGTACGTACGCGCACGCGGGGGCGTTTGTGGAGAAGAAAGGTGTGCCCTC GGTGCCCGTGGCAGAGGAGAGCCGTGCCAGCGCACTGCCCTACGTGCTCACCGACATCGCGGCCTCCTTC ACCCTGCTGGTTGGCATCTACTTCCCCTCCGTGACCGGTATCATGGCGGGTTCAAACCGGTCCGGGGACC TCAAGGATGCACAGAAGTCCATCCCCACGGGGACCATCCTGGCCATAGTGACGACGTCTTTCATCTATCT CTCCTGCATTGTGCTGTTTGGGGCCTGCATTGAAGGCGTGGTCTTACGAGATAAGTTCGGGGAGGCCCTG CAGGGGAACCTGGTCATCGGCATGCTGGCCTGGCCCTCCCCCTGGGTCATCGTCATCGGCTCCTTCTTCT CCACCTGCGGTGCCGGCCTGCAGAGCCTCACGGGGGCACCGCGCCTACTGCAGGCCATTGCCCGTGACGG CATCGTCCCCTTCCTGCAGGTGTTTGGCCACGGGAAGGCCAACGGGGAGCCCACGTGGGCGCTGCTGCTG ACAGTCCTCATCTGCGAGACTGGCATCCTCATCGCCTCTCTGGACAGCGTGGCCCCGATCCTCTCCATGT TCTTCCTCATGTGCTACCTGTTCGTGAACCTGGCCTGCGCCGTGCAGACCCTGCTACGTACCCCCAACTG GCGTCCACGCTTCAAGTTCTACCACTGGACCCTGTCCTTTCTGGGTATGAGCCTGTGCCTGGCGCTGATG TTCATCTGCTCCTGGTACTACGCGCTGTCCGCCATGCTCATCGCTGGCTGCATCTACAAGTACATCGAGT ACCGCGGGGCCGAGAAGGAGTGGGGCGATGGCATCCGTGGCCTATCCCTGAACGCCGCCCGCTACGCCCT GCTGCGCGTGGAGCACGGTCCCCCCCACACCAAGAACTGGAGGCCCCAGGTGCTGGTGATGCTGAACCTG GACGCGGAGCAGGCCGTGAAGCACCCCCGCCTGCTGTCCTTCACGTCGCAGCTGAAGGCCGGCAAGGGCC TGACCATCGTGGGCTCGGTGCTGGAGGGGACGTACCTGGACAAGCACATGGAGGCTCAGCGGGCCGAGGA GAACATACGGTCCCTAATGAGCACAGAGAAGACCAAGGGCTTCTGCCAGCTGGTGGTCTCGTCCAGCCTG CGGGATGGCATGTCCCACCTGATCCAGTCGGCCGGCCTGGGCGGCCTGAAGCACAACACGGTGCTCATGG CCTGGCCCGCATCCTGGAAGCAGGAGGACAACCCCTTCTCCTGGAAGAACTTTGTGGACACCGTCCGCGA CACCACCGCCGCGCACCAGGCTCTGCTGGTGGCCAAGAACGTCGACTCGTTTCCGCAAAACCAGGAGCGC TTCGGCGGGGGCCACATCGACGTGTGGTGGATCGTGCACGACGGCGGCATGCTCATGCTGCTGCCCTTCC TGCTGCGCCAGCACAAGGTGTGGAGGAAGTGCCGGATGCGTATCTTCACCGTGGCCCAGGTGGACGACAA CAGCATCCAGATGAAGAAGGACCTGCAGATGTTCTTGTATCACTTGCGCATCAGCGCCGAGGTGGAGGTG GTGGAGATGGTTGAAAACGACATATCTGCTTTCACCTACGAGAGGACACTAATGATGGAGCAGAGGTCGC AGATGCTGAAGCAGATGCAGCTGTCCAAGAACGAGCAGGAGCGAGAGGCCCAGCTGATCCACGACAGGAA CACCGCGTCCCACACCGCGGCGGCAGCCAGGACCCAAGCGCCGCCTACGCCAGACAAGGTGCAGATGACC TGGACCAGGGAGAAGCTGATCGCTGAGAAGTACAGGAGCAGAGACACCAGCCTATCCGGTTTCAAAGACC TCTTCAGCATGAAGCCGGACCAGTCCAACGTCAGGCGGATGCACACGGCTGTGAAGCTCAATGGCGTCGT CCTCAACAAGTCCCAGGATGCGCAGCTGGTCCTGCTCAACATGCCAGGTCCTCCCAAAAACCGGCAGGGA GACGAGAACTACATGGAGTTTCTTGAAGTCCTGACCGAGGGGCTGAACAGAGTCCTCCTGGTCAGGGGTG GCGGCCGGGAGGTGATCACCATCTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210512 representing NM_006598
Red=Cloning site Green=Tags(s) MPTNFTVVPVEAHADGGGDETAERTEAPGTPEGPEPERPSPGDGNPRENSPFLNNVEVEQESFFEGKNMA LFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEESRRREAKAPRMGTFIGVYLPCLQNILGVILFLR LTWIVGVAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTT FAGAMYILGTIEIFLTYISPGAAIFQAEAAGGEAAAMLHNMRVYGTCTLVLMALVVFVGVKYVNKLALVF LACVVLSILAIYAGVIKSAFDPPDIPVCLLGNRTLSRRSFDACVKAYGIHNNSATSALWGLFCNGSQPSA ACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAHAGAFVEKKGVPSVPVAEESRASALPYVLTDIAASF TLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEAL QGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLL TVLICETGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALM FICSWYYALSAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNL DAEQAVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHMEAQRAEENIRSLMSTEKTKGFCQLVVSSSL RDGMSHLIQSAGLGGLKHNTVLMAWPASWKQEDNPFSWKNFVDTVRDTTAAHQALLVAKNVDSFPQNQER FGGGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEV VEMVENDISAFTYERTLMMEQRSQMLKQMQLSKNEQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMT WTREKLIAEKYRSRDTSLSGFKDLFSMKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKNRQG DENYMEFLEVLTEGLNRVLLVRGGGREVITIYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006598 |
| ORF Size | 3249 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006598.3 |
| RefSeq Size | 5239 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 10723 |
| UniProt ID | Q9Y666 |
| Protein Families | Transmembrane |
| MW | 118.9 kDa |
| Gene Summary | Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SLC12A7 alters adrenocortical carcinoma cell adhesion properties to promote an aggressive invasive behavior
,Brown, TC;Murtha, TD;Rubinstein, JC;Korah, R;Carling, T;,
Cell Commun. Signal
,PubMed ID 29884238
[KCC4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210512L1 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged |
CNY 9,944.00 |
|
| RC210512L2 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged |
CNY 12,730.00 |
|
| RC210512L3 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC210512L4 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged |
CNY 12,730.00 |
|
| RG210512 | SLC12A7 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) |
CNY 11,880.00 |
|
| SC303797 | SLC12A7 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) |
CNY 7,552.00 |
