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SELS (SELENOS) (NM_203472) Human Tagged ORF Clone
CAT＃: RC210475
- TrueORF®
VIMP (Myc-DDK-tagged)-Human selenoprotein S (SELS), transcript variant 1, (Note, selenocysteine protein, internal stop codon, see reference data summary)
ORF Plasmid: tGFP
"NM_203472" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Untagged ORF Clone |
| Synonyms | AD-015; ADO15; SBBI8; SELS; SEPS1; VIMP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210475 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACGCCAAGAGGAGTCTCTGTCCGCGCGGCCGGCCCTGGAGACCGAGGGGCTGCGCTTCCTGCACA CCACGGTGGGCTCCCTGCTGGCCACCTATGGCTGGTACATCGTCTTCAGCTGCATCCTTCTCTACGTGGT CTTTCAGAAGCTTTCCGCCCGGCTAAGAGCCTTGAGGCAGAGGCAGCTGGACCGAGCTGCGGCTGCTGTG GAACCTGATGTTGTTGTTAAACGACAAGAAGCTTTAGCAGCTGCTCGACTGAAAATGCAAGAAGAACTAA ATGCGCAAGTTGAAAAGCATAAGGAAAAACTGAAACAACTTGAAGAAGAAAAAAGGAGACAGAAGATTGA AATGTGGGACAGCATGCAAGAAGGAAAAAGTTACAAAGGAAATGCAAAGAAGCCCCAGGAGGAAGACAGT CCTGGGCCTTCCACTTCATCTGTCCTGAAACGGAAATCGGACAGAAAGCCTTTGCGGGGAGGAGGTTATA ACCCGTTGTCTGGTGAAGGAGGCGGAGCTTGCTCCTGGAGACCTGGACGCAGAGGCCCGTCATCTGGCGG ATGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210475 protein sequence
Red=Cloning site Green=Tags(s) MERQEESLSARPALETEGLRFLHTTVGSLLATYGWYIVFSCILLYVVFQKLSARLRALRQRQLDRAAAAV EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEEDS PGPSTSSVLKRKSDRKPLRGGGYNPLSGEGGGACSWRPGRRGPSSGG*G TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203472 |
| ORF Size | 567 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_203472.1, NP_982298.1 |
| RefSeq Size | 1417 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 55829 |
| UniProt ID | Q9BQE4 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a transmembrane protein that is localized in the endoplasmic reticulum (ER). It is involved in the degradation process of misfolded proteins in the ER, and may also have a role in inflammation control. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Two additional phylogenetically conserved stem-loop structures (Stem-loop 1 and Stem-loop 2) in the 3' UTR of this mRNA have been shown to function as modulators of Sec insertion. An alternatively spliced transcript variant, lacking the SECIS element and encoding a non-Sec containing shorter isoform, has been described for this gene (PMID:23614019). [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210475L1 | Lenti-ORF, VIMP (Myc-DDK-tagged)-Human selenoprotein S (SELS), transcript variant 1, (Note, selenocysteine protein, internal stop codon, see summary) |
CNY 4,800.00 |
|
| RG210475 | VIMP (GFP-tagged) - Human selenoprotein S (SELS), transcript variant 1, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 4,000.00 |
|
| SC308201 | VIMP (untagged)-Human selenoprotein S (SELS), transcript variant 1 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,990.00 |
|
| SC321028 | VIMP (untagged)-Human selenoprotein S (SELS), transcript variant 1 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,696.00 |
