COLQ (NM_005677) Human Tagged ORF Clone
CAT#: RC210401
COLQ (Myc-DDK-tagged)-Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I
ORF Plasmid: tGFP
"NM_005677" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CMS5; EAD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210401 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGC CGACTTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGG CCACAAAGCATGCTGCCTGCTGACGCCTCCTCCACCACCACTGTTCCCACCACCATTCTTCAGAGGTGGC CGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCGTGCA TGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGG ACCAAAGGGAGAAAAGGGGGAGCTTGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCT GGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTATGA TGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATC CAGAGGTGAAAAGGGTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGG CAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCCACAGGAAGACCAG GAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTC TGGTCAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGG GAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATAACCCTTCCT ACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGA GCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGAC AGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCA CCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTG CATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCT GACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACT GCTACATCGACTCCACGCCCTGCCGCTACTTCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210401 protein sequence
Red=Cloning site Green=Tags(s) MVVLNPMTLGIYLQLFFLSIVSQPTFINSVLPISAALPSLDQKKRGGHKACCLLTPPPPPLFPPPFFRGG RSPLLSPDMKNLMLELETSQSPCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGELGRPGRKGRPGPPGVP GMPGPIGWPGPEGPRGEKGDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEKGDLGPKGEKGFPGFPGMLG QKGEMGPKGEPGIAGHRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSGQPGRPGPPGPPPAGQLIMGPKG ERGFPGPPGRCLCGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQEELERLNTQNAIAFRRDQRSLYFKD SLGWLPIQLTPFYPVDYTADQHGTCGDGLLQPGEECDDGNSDVGDDCIRCHRAYCGDGHRHEGVEDCDGS DFGYLTCETYLPGSYGDLQCTQYCYIDSTPCRYFT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_005677 |
ORF Size | 1365 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005677.4 |
RefSeq Size | 3007 bp |
RefSeq ORF | 1368 bp |
Locus ID | 8292 |
UniProt ID | Q9Y215 |
Protein Families | Druggable Genome, Transmembrane |
MW | 47.8 kDa |
Gene Summary | This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210401L1 | Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210401L2 | Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mGFP tagged |
CNY 5,890.00 |
|
RC210401L3 | Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210401L4 | Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mGFP tagged |
CNY 5,890.00 |
|
RG210401 | COLQ (tGFP-tagged) - Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I |
CNY 5,256.00 |
|
SC303680 | COLQ (untagged)-Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I |
CNY 3,656.00 |