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5HT4 Receptor (HTR4) (NM_000870) Human Tagged ORF Clone
CAT＃: RC210367
HTR4 (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b
ORF Plasmid: tGFP
"NM_000870" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HT4; 5-HT4R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210367 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCA CGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGGGA CAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCG GTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTC TTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGCTGCATTTCTCTGGA TAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTA ATGCTGGGAGGCTGCTGGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACA TTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCAT GGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTG GCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAG CCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCATAGCACTCATCGCATGAGGACAGAGACCAAAGC AGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATT GTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCA ATTCCGGGTTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCT CTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGTTCAACCACAACC ATTAATGGATCCACACATGTACTAAGGGATGCAGTGGAGTGTGGTGGCCAGTGGGAGAGTCAGTGTCACC CGCCAGCAACTTCTCCTTTGGTGGCTGCTCAGCCCAGTGACACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210367 protein sequence
Red=Cloning site Green=Tags(s) MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVS VLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIAL MLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVL AYYRIYVTAKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNI VDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTT INGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000870 |
| ORF Size | 1164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000870.7 |
| RefSeq Size | 3331 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 3360 |
| UniProt ID | Q13639 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| MW | 43.8 kDa |
| Gene Summary | This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210367L3 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210367L4 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
| RG210367 | HTR4 (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b |
CNY 5,256.00 |
|
| SC310455 | HTR4 (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b |
CNY 3,656.00 |
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