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PON1 (NM_000446) Human Tagged ORF Clone
CAT＃: RC210356
PON1 (Myc-DDK-tagged)-Human paraoxonase 1 (PON1)
ORF Plasmid: tGFP
"NM_000446" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ESA; MVCD5; PON |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210356 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGCTGATTGCGCTCACCCTCTTGGGGATGGGACTGGCACTCTTCAGGAACCACCAGTCTTCTT ACCAAACACGACTTAATGCTCTCCGAGAGGTACAACCCGTAGAACTTCCTAACTGTAATTTAGTTAAAGG AATCGAAACTGGCTCTGAAGACTTGGAGATACTGCCTAATGGACTGGCTTTCATTAGCTCTGGATTAAAG TATCCTGGAATAAAGAGCTTCAACCCCAACAGTCCTGGAAAAATACTTCTGATGGACCTGAATGAAGAAG ATCCAACAGTGTTGGAATTGGGGATCACTGGAAGTAAATTTGATGTATCTTCATTTAACCCTCATGGGAT TAGCACATTCACAGATGAAGATAATGCCATGTACCTCCTGGTGGTGAACCATCCAGATGCCAAGTCCACA GTGGAGTTGTTTAAATTTCAAGAAGAAGAAAAATCGCTTTTGCATCTAAAAACCATCAGACATAAACTTC TGCCTAATTTGAATGATATTGTTGCTGTGGGACCTGAGCACTTTTATGGCACAAATGATCACTATTTTCT TGACCCCTACTTACAATCCTGGGAGATGTATTTGGGTTTAGCGTGGTCGTATGTTGTCTACTATAGTCCA AGTGAAGTTCGAGTGGTGGCAGAAGGATTTGATTTTGCTAATGGAATCAACATTTCACCCGATGGCAAGT ATGTCTATATAGCTGAGTTGCTGGCTCATAAGATTCATGTGTATGAAAAGCATGCTAATTGGACTTTAAC TCCATTGAAGTCCCTTGACTTTAATACCCTCGTGGATAACATATCTGTGGATCCTGAGACAGGAGACCTT TGGGTTGGATGCCATCCCAATGGCATGAAAATCTTCTTCTATGACTCAGAGAATCCTCCTGCATCAGAGG TGCTTCGAATCCAGAACATTCTAACAGAAGAACCTAAAGTGACACAGGTTTATGCAGAAAATGGCACAGT GTTGCAAGGCAGTACAGTTGCCTCTGTGTACAAAGGGAAACTGCTGATTGGCACAGTGTTTCACAAAGCT CTTTACTGTGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210356 protein sequence
Red=Cloning site Green=Tags(s) MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLK YPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKST VELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSP SEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDL WVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKA LYCEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000446 |
| ORF Size | 1065 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000446.7 |
| RefSeq Size | 1769 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 5444 |
| UniProt ID | P27169 |
| Domains | Arylesterase |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Metabolic pathways |
| MW | 39.7 kDa |
| Gene Summary | This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210356L1 | Lenti ORF clone of Human paraoxonase 1 (PON1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210356L2 | Lenti ORF clone of Human paraoxonase 1 (PON1), mGFP tagged |
CNY 5,890.00 |
|
| RC210356L3 | Lenti ORF clone of Human paraoxonase 1 (PON1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210356L4 | Lenti ORF clone of Human paraoxonase 1 (PON1), mGFP tagged |
CNY 5,890.00 |
|
| RG210356 | PON1 (tGFP-tagged) - Human paraoxonase 1 (PON1) |
CNY 5,256.00 |
|
| SC119866 | PON1 (untagged)-Human paraoxonase 1 (PON1) |
CNY 3,656.00 |
