NBPF9 (NM_001037675) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AE01
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC210283 ORF sequence, codon optimized. Due to the complexity of NM_001037675, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTGTCTGCTGGTCCCTGGTCTAGCGAGAAGGCTGAAATGAACATTCTGGAGATCAATGAGAAAC TTCGGCCTCAGTTGGCTGAAAACAAGCAGCAGTTCGGAAACCTGAAGGAGCGGTGCTTTCTGACCCAGCT GGCCGGATTCCTGGCCAACCGGCAAAAGAAGTATAAATATGAGGAGTGTAAAGACCTGATTAAATTTATG CTGAGAAACGAGAGACAGTTCAAAGAGGAAAAGCTCGCCGAACAGTTGAAGCAGGCTGAAGAACTCCGCC AGTACAAAGTTCTGGTTCATTCACAGGAGCGGGAGCTCACGCAGCTCAAGGAAAAACTTCGGGAGGGAAG AGATGCCTCTCGCTCCCTCAATGAGCACCTGCAAGCACTGCTTACTCCCGACGAGCCCGATAAGTCCCAG GGACAAGACCTCCAAGAGCAGCTGGCTGAAGGATGCCGGTTGGCTCAACACCTGGTCCAAAAGCTGAGCC CGGAGAATGATGAAGATGAGGACGAAGATGTGCAGGTGGAAGAGGATGAAAAGGTGCTCGAGTCACCTGC TCCCCGCGAAGTCCAAAAGGCCGAGGAATCTAAAGTTACTGAGGACTCCCTTGAGGAGTGCGCAATAACA TGTAGCAACAGTCACGGCCCCTGCGATTCCAATCAGCCTCACAAGAACATAAAAATTACATTCGAAGAGG ACGAGGTGAACTCAACTCTGGTGGTGGACCGCGAAAGCTCCCACGACGAGTGTCAAGATGCCTTGAACAT CTTGCCTGTTCCTGGACCTACCAGTTCCGCAACTAACGTCAGTATGGTGGTTTCCGCGGGCCCTCTCTCT TCTGAGAAGGCCGAGATGAATATCCTGGAGATCAATGAGAAGTTGCGACCTCAGTTGGCAGAGAAGAAAC AGCAATTCCGCAATCTCAAGGAGAAATGTTTTCTGACCCAGCTCGCAGGGTTCCTTGCCAATCAGCAAAA CAAGTATAAGTACGAGGAATGTAAGGACCTGATCAAATTCATGCTGAGAAATGAACGGCAGTTTAAAGAA GAGAAACTCGCCGAGCAGCTGAAGCAGGCAGAGGAGCTTAGGCAGTATAAAGTCCTTGTTCATGCCCAGG AAAGAGAGCTCACCCAACTGCGCGAGAAGCTGAGGGAAGGCAGGGACGCATCCCGCTCTCTTAACGAGCA TCTGCAGGCCCTTCTGACACCTGATGAGCCAGATAAGTCACAGGGCCAAGACCTGCAGGAGCAACTCGCT GAAGGCTGCCGACTCGCCCAACATTTGGTACAGAAGCTGTCTCCAGAAAATGATAACGACGACAATGAGG ATGTGCAGGTAGAAGTGGCAGAAAAGGTGCAGAAATCTAGTGCGCCACGAGAGATGCAGAAAGCTGAGGA GAAAGAAGTACCAGAGGATAGCCTCGAGGAGTGCGCAATCACCTATAGCAATAGCCACGGTCCTTATGAT TCTAATCAACCACACAGAAAGACCAAAATCACGTTCGAGGAAGATAAAGTGGACTCCACACTCATCGGGA GTTCCTCCCACGTGGAGAGGGAGGATGCAGTGCACATAATCCCTGAGAACGAGTCCGACGATGAGGAAGA AGAGGAGAAAGGACCAGTTAGTCCTAGAAATCTCCAGGAGTCAGAAGAAGAGGAGGTTCCACAGGAATCT TGGGATGAGGGCTACTCAACTCCTAGCATACCCCCCGAGATGCTGGCCTCCTATAAGTCATATTCTAGTA CATTCCACTCCCTCGAGGAGCAGCAGGTCTGTATGGCCGTTGATATTGGCAGGCATAGGTGGGATCAGGT GAAAAAAGAAGACCAAGAAGCGACCGGGCCCCGGCTGAGCCGCGAACTCCTTGACGAGAAAGGCCCTGAA GTGTTGCAAGATAGCCTCTACCGGTGCTACAGCACCCCAAGCGGGTGCCTGGAGCTCACAGATAGTTGCC AACCTTACCGAAGCGCTTTTTACGTACTCGAGCAGCAGAGGGTCGGACTGGCCGTCGATATGGATGAGAT CGAAAAATACCAGGAGGTTGAAGAGGATCAGGATCCTAGTTGCCCGAGACTCTCCCGCGAACTGCTTGAT GAGAAAGAACCCGAAGTGCTGCAGGATTCTCTGGGCCGGTGGTACTCTACGCCCTCTGGATATCCTGAGC TTCCAGACCTTGGTCAACCTTACTCATCTGCAGTGTATAGTCTCGAGGAACAGTACCTGGGACTCGCTCT CGACCTCGATAGAATAAAGAAAGACCAGGAAGAAGAGGAAGATCAGGGCCCACCCTGCCCTCGGCTGAGC CGGGAGCTCCTGGAGGTGGTCGAACCTGAAGTCCTGCAGGACAGTCTGGATCGCTGCTATTCAACGCCCA GCTCCTGTCTTGAACAGCCCGATTCTTGGCAGCCTTACGGGAGTTCTTTTTATGCACTCGAGGAGAAACA TGTGGGGTTCTCACTCGATGTCGGGGAGATTGAAAAAAAAGGAAAGGGAAAAAAACGCCGGGGCCGACGG AGCAAGAAGGAGAGGCGGCGAGGCCGGAAGGAAGGGGAAGAAGACCAGAATCCCCCTTGTCCACGGCTGA ATGGCGTTCTGATGGAAGTCGAAGAGCCCGAAGTTCTCCAGGATAGCCTTGATGGTTGCTATTCCACTCC TTCAATGTACTTTGAGCTTCCTGACAGCTTTCAGCATTATCGCAGCGTTTTTTACTCATTTGAGGAGCAG CACATTTCCTTCGCCCTGTACGTGGACAACCGGTTTTTCACCCTGACGGTGACCAGCTTGCACCTGGTTT TCCAGATGGAAGTGATATTTCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC210283 representing NM_001037675 Red=Cloning site Green=Tags(s) MVVSAGPWSSEKAEMNILEINEKLRPQLAENKQQFGNLKERCFLTQLAGFLANRQKKYKYEECKDLIKFM LRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLKEKLREGRDASRSLNEHLQALLTPDEPDKSQ GQDLQEQLAEGCRLAQHLVQKLSPENDEDEDEDVQVEEDEKVLESPAPREVQKAEESKVTEDSLEECAIT CSNSHGPCDSNQPHKNIKITFEEDEVNSTLVVDRESSHDECQDALNILPVPGPTSSATNVSMVVSAGPLS SEKAEMNILEINEKLRPQLAEKKQQFRNLKEKCFLTQLAGFLANQQNKYKYEECKDLIKFMLRNERQFKE EKLAEQLKQAEELRQYKVLVHAQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLA EGCRLAQHLVQKLSPENDNDDNEDVQVEVAEKVQKSSAPREMQKAEEKEVPEDSLEECAITYSNSHGPYD SNQPHRKTKITFEEDKVDSTLIGSSSHVEREDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQES WDEGYSTPSIPPEMLASYKSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDQEATGPRLSRELLDEKGPE VLQDSLYRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLD EKEPEVLQDSLGRWYSTPSGYPELPDLGQPYSSAVYSLEEQYLGLALDLDRIKKDQEEEEDQGPPCPRLS RELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSWQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRR SKKERRRGRKEGEEDQNPPCPRLNGVLMEVEEPEVLQDSLDGCYSTPSMYFELPDSFQHYRSVFYSFEEQ HISFALYVDNRFFTLTVTSLHLVFQMEVIFPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001037675
ORF Size	2826 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001037675.1, NM_001037675.2, NM_001037675.3, NP_001032764.1
RefSeq Size	4527 bp
RefSeq ORF	2829 bp
Locus ID	400818
UniProt ID	P0DPF3
MW	108.6 kDa
Gene Summary	This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. [provided by RefSeq, Apr 2013]

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