MARCKS (NM_002356) Human Tagged ORF Clone
CAT#: RC210277
- TrueORF®
MARCKS (Myc-DDK-tagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS)
ORF Plasmid: tGFP
"NM_002356" in other vectors (6)
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CNY 3,600.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 80K-L; MACS; PKCSL; PRKCSL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210277 representing NM_002356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCCCAGTTCTCCAAGACCGCAGCGAAGGGAGAAGCCGCCGCGGAGAGGCCTGGGGAGGCGGCTG TGGCCTCGTCGCCTTCCAAAGCGAACGGACAGGAGAATGGCCACGTGAAGGTAAACGGCGACGCTTCGCC CGCGGCCGCCGAGTCGGGCGCCAAGGAGGAGCTGCAGGCCAACGGCAGCGCCCCGGCCGCCGACAAGGAG GAGCCCGCGGCCGCCGGGAGCGGGGCGGCGTCGCCCTCCGCGGCCGAGAAAGGTGAGCCGGCCGCCGCCG CTGCCCCCGAGGCCGGGGCCAGCCCGGTAGAGAAGGAGGCCCCCGCGGAAGGCGAGGCTGCCGAGCCCGG CTCGCCCACGGCCGCGGAGGGAGAGGCCGCGTCGGCCGCCTCCTCGACTTCTTCGCCCAAGGCCGAGGAC GGGGCCACGCCCTCGCCCAGCAACGAGACCCCGAAAAAAAAAAAGAAGCGCTTTTCCTTCAAGAAGTCTT TCAAGCTGAGCGGCTTCTCCTTCAAGAAGAACAAGAAGGAGGCTGGAGAAGGCGGTGAGGCTGAGGCGCC CGCTGCCGAAGGCGGCAAGGACGAGGCCGCCGGGGGCGCAGCTGCGGCCGCCGCCGAGGCGGGCGCGGCC TCCGGGGAGCAGGCAGCGGCGCCGGGCGAGGAGGCGGCAGCGGGCGAGGAGGGGGCGGCGGGTGGCGACC CGCAGGAGGCCAAGCCCCAGGAGGCCGCTGTCGCGCCAGAGAAGCCGCCCGCCAGCGACGAGACCAAGGC CGCCGAGGAGCCCAGCAAGGTGGAGGAGAAAAAGGCCGAGGAGGCCGGGGCCAGCGCCGCCGCCTGCGAG GCCCCCTCCGCCGCCGGGCCCGGCGCGCCCCCGGAGCAGGAGGCAGCCCCCGCGGAGGAGCCCGCGGCCG CCGCAGCCTCGTCAGCCTGCGCAGCCCCCTCACAGGAGGCCCAGCCCGAGTGCAGTCCAGAAGCCCCCCC AGCGGAGGCGGCAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210277 representing NM_002356
Red=Cloning site Green=Tags(s) MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKE EPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAED GATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAA SGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACE APSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002356 |
ORF Size | 996 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002356.7 |
RefSeq Size | 2589 bp |
RefSeq ORF | 999 bp |
Locus ID | 4082 |
UniProt ID | P29966 |
Domains | MARCKS |
Protein Families | Druggable Genome |
Protein Pathways | Fc gamma R-mediated phagocytosis |
MW | 31.4 kDa |
Gene Summary | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210277L1 | Lenti-ORF clone of MARCKS (Myc-DDK-tagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 6,000.00 |
|
RC210277L2 | Lenti-ORF clone of MARCKS (mGFP-tagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 5,890.00 |
|
RC210277L3 | Lenti-ORF clone of MARCKS (Myc-DDK-tagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 5,890.00 |
|
RC210277L4 | Lenti-ORF clone of MARCKS (mGFP-tagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 5,890.00 |
|
RG210277 | MARCKS (tGFP-tagged) - Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 5,200.00 |
|
SC125313 | MARCKS (untagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS) |
CNY 3,600.00 |