RGS8 (NM_033345) Human Tagged ORF Clone
CAT#: RC210236
RGS8 (Myc-DDK-tagged)-Human regulator of G-protein signaling 8 (RGS8), transcript variant 1
ORF Plasmid: tGFP
"NM_033345" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210236 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAACACCTTAACCCGAAGCCTCTCTGACCATCCAGTTGGCAAAGACCCTCAGGCCATGAGGACTG GCCAAAGACAGAACAAAGGGATGAGGACTCGACTGGGATGCCTGTCTCACAAGTCAGACTCGTGTAGTGA TTTCACAGCTATTCTTCCAGACAAACCCAACCGCGCTCTCAAGAGATTATCGACAGAAGAAGCTACGAGG TGGGCAGATTCCTTTGATGTGCTTCTCTCTCATAAGTATGGGGTGGCTGCATTCCGTGCCTTCTTGAAGA CGGAGTTCAGTGAGGAGAACCTGGAATTCTGGTTGGCCTGTGAGGAGTTCAAGAAGACCAGGTCCACTGC AAAACTGGTCTCTAAGGCCCATAGGATCTTTGAGGAGTTTGTGGATGTGCAGGCTCCACGGGAGGTAAAC ATTGACTTCCAGACCCGAGAAGCCACGAGGAAGAACCTGCAGGAGCCATCCCTGACTTGCTTTGACCAAG CCCAAGGAAAAGTACACAGCCTCATGGAGAAAGACTCTTACCCCAGGTTCCTGAGGTCCAAAATGTACTT AGATCTGCTGTCCCAAAGCCAGAGGAGGCTCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210236 protein sequence
Red=Cloning site Green=Tags(s) MWNTLTRSLSDHPVGKDPQAMRTGQRQNKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATR WADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVN IDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_033345 |
ORF Size | 594 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033345.3 |
RefSeq Size | 783 bp |
RefSeq ORF | 597 bp |
Locus ID | 85397 |
UniProt ID | P57771 |
Domains | RGS |
Protein Families | Druggable Genome |
MW | 23 kDa |
Gene Summary | This gene is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210236L3 | Lenti ORF clone of Human regulator of G-protein signaling 8 (RGS8), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210236L4 | Lenti ORF clone of Human regulator of G-protein signaling 8 (RGS8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG210236 | RGS8 (tGFP-tagged) - Human regulator of G-protein signaling 8 (RGS8), transcript variant 1 |
CNY 4,000.00 |
|
SC310633 | RGS8 (untagged)-Human regulator of G-protein signaling 8 (RGS8), transcript variant 1 |
CNY 2,400.00 |