SLC7A2 (NM_001008539) Human Tagged ORF Clone

CAT#: RC210214

SLC7A2 (Myc-DDK-tagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (SLC7A2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001008539" in other vectors (4)

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CNY 4,896.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SLC7A2 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ATRC2; CAT2; HCAT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210214 representing NM_001008539
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCTTGCAGAGCCGCGCTGACCTTTGCCCGATGTCTGATCCGGAGAAAAATCGTGACCCTGGACA
GTCTAGAAGACACCAAATTATGCCGCTGCTTATCCACCATGGACCTCATTGCCCTGGGCGTTGGAAGCAC
CCTTGGGGCCGGGGTTTATGTCCTCGCTGGGGAGGTGGCCAAGGCAGACTCGGGCCCCAGCATCGTGGTG
TCCTTCCTCATTGCTGCCCTGGCTTCAGTGATGGCTGGCCTCTGCTATGCCGAATTTGGGGCCCGTGTTC
CCAAGACGGGGTCTGCATATTTGTACACCTACGTGACTGTCGGAGAGCTGTGGGCCTTCATCACTGGCTG
GAATCTCATTTTATCGTATGTGATAGGTACATCAAGTGTTGCAAGAGCCTGGAGTGGCACCTTTGATGAA
CTTCTTAGCAAACAGATTGGTCAGTTTTTGAGGACATACTTCAGAATGAATTACACTGGTCTTGCAGAAT
ATCCCGATTTTTTTGCTGTGTGCCTTATATTACTTCTAGCAGGTCTTTTGTCTTTTGGAGTAAAAGAGTC
TGCTTGGGTGAATAAAGTCTTCACAGCTGTTAATATTCTCGTCCTTCTGTTTGTGATGGTTGCTGGGTTT
GTGAAAGGAAATGTGGCAAACTGGAAGATTAGTGAAGAGTTTCTCAAAAATATATCAGCAAGTGCCAGAG
AGCCACCTTCTGAAAACGGAACAAGTATCTATGGGGCTGGTGGCTTTATGCCTTATGGCTTTACGGGAAC
GTTGGCTGGTGCTGCAACTTGCTTTTATGCCTTTGTGGGATTTGACTGCATTGCAACAACTGGTGAAGAA
GTTCGGAATCCCCAGAAAGCTATTCCCATTGGAATTGTGACGTCTTTGCTTGTTTGCTTTATGGCCTATT
TTGGGGTCTCTGCAGCTTTAACACTTATGATGCCGTACTACCTCCTCGATGAAAAAAGCCCCCTTCCTGT
AGCGTTTGAATATGTGGGATGGGGTCCTGCCAAATATGTCGTCGCAGCTGGTTCTCTCTGCGCCTTGTCA
ACAAGTCTTCTTGGATCCATTTTCCCAATGCCTCGTGTAATCTATGCTATGGCGGAGGATGGGTTGCTTT
TCAAATGTCTAGCTCAAATCAATTCCAAAACGAAGACACCAATAATTGCTACTTTATCATCGGGTGCAGT
GGCAGCTTTGATGGCCTTTCTGTTTGACCTGAAGGCGCTTGTGGACATGATGTCCATTGGCACCCTCATG
GCCTACTCTCTGGTGGCAGCCTGTGTTCTCATCCTCAGGTACCAGCCTGGCTTATCTTACGACCAGCCCA
AATGTTCTCCTGAGAAAGATGGTCTGGGATCGTCTCCCAGGGTAACCTCGAAGAGTGAGTCCCAGGTCAC
CATGCTGCAGAGACAGGGCTTCAGCATGCGGACCCTCTTCTGCCCCTCCCTTCTGCCAACACAGCAGTCA
GCTTCTCTCGTGAGCTTTCTGGTAGGATTCCTAGCTTTCCTCGTGTTGGGCCTGAGTGTCTTGACCACTT
ACGGAGTTCATGCCATCACCAGGCTGGAGGCCTGGAGCCTCGCTCTCCTCGCGCTGTTTCTTGTTCTCTT
CGTTGCCATCGTTCTCACCATCTGGAGGCAGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTTCCATTC
TTACCATTTTTGCCAGCGTTCAGCATCTTGGTGAACATTTACTTGATGGTCCAGTTAAGTGCAGACACTT
GGGTCAGATTCAGCATTTGGATGGCAATTGGCTTCCTGATTTACTTTTCTTATGGCATTAGACACAGCCT
GGAGGGTCATCTGAGAGATGAAAACAATGAAGAAGATGCTTATCCAGACAACGTTCATGCAGCAGCAGAA
GAAAAATCTGCCATTCAAGCAAATGACCATCACCCAAGAAATCTCAGTTCACCTTTCATATTCCATGAAA
AGACAAGTGAATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210214 representing NM_001008539
Red=Cloning site Green=Tags(s)

MIPCRAALTFARCLIRRKIVTLDSLEDTKLCRCLSTMDLIALGVGSTLGAGVYVLAGEVAKADSGPSIVV
SFLIAALASVMAGLCYAEFGARVPKTGSAYLYTYVTVGELWAFITGWNLILSYVIGTSSVARAWSGTFDE
LLSKQIGQFLRTYFRMNYTGLAEYPDFFAVCLILLLAGLLSFGVKESAWVNKVFTAVNILVLLFVMVAGF
VKGNVANWKISEEFLKNISASAREPPSENGTSIYGAGGFMPYGFTGTLAGAATCFYAFVGFDCIATTGEE
VRNPQKAIPIGIVTSLLVCFMAYFGVSAALTLMMPYYLLDEKSPLPVAFEYVGWGPAKYVVAAGSLCALS
TSLLGSIFPMPRVIYAMAEDGLLFKCLAQINSKTKTPIIATLSSGAVAALMAFLFDLKALVDMMSIGTLM
AYSLVAACVLILRYQPGLSYDQPKCSPEKDGLGSSPRVTSKSESQVTMLQRQGFSMRTLFCPSLLPTQQS
ASLVSFLVGFLAFLVLGLSVLTTYGVHAITRLEAWSLALLALFLVLFVAIVLTIWRQPQNQQKVAFMVPF
LPFLPAFSILVNIYLMVQLSADTWVRFSIWMAIGFLIYFSYGIRHSLEGHLRDENNEEDAYPDNVHAAAE
EKSAIQANDHHPRNLSSPFIFHEKTSEF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001008539
ORF Size 1974 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001008539.4
RefSeq Size 7389 bp
RefSeq ORF 1977 bp
Locus ID 6542
UniProt ID P52569
Protein Families Druggable Genome, Transmembrane
MW 71.5 kDa
Gene Summary The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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