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EDDM3A (NM_006683) Human Tagged ORF Clone
CAT＃: RC210112
- TrueORF®
EDDM3A (Myc-DDK-tagged)-Human epididymal protein 3A (EDDM3A)
ORF Plasmid: tGFP
"NM_006683" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EP3A; FAM12A; HE3-ALPHA; HE3A; HE3ALPHA; RAM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210112 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCCTCTCTAAAGATTTGGGGCATACTCTTGGCCCTGCTTTGCATCCTTTGCAGGCTGTGTGTAT ACAGTAACAACATTTACTGGAGAGAATTCATAAAACTTCATTACTTAAGTCCAAGTCGAGAATTCAAAGA GTACAAATGTGATGTCCTCATGAGAGAAAAAGAGGCTCTGAAAGGCAAGAGCTTTCATATGTTCATCTAT AGCTTATGGTTCAAAATTCAGCGTGCATGCATCAATGAGAAGGGGAGTGACCGATATAGAAATGCATATG TATGGGCCCCAGGTGCCCTCAAAGTACTCGAGTGTCACTGGGAGAAGTACAACAATAGGTACACAGAGAG CAGAAGCTTCAGCTACATTGAATTCCATTGTGGCGTAGATGGATATGTTGATAACATAGAAGACCTGAGG ATTATAGAACCTATCAGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210112 protein sequence
Red=Cloning site Green=Tags(s) MTSSLKIWGILLALLCILCRLCVYSNNIYWREFIKLHYLSPSREFKEYKCDVLMREKEALKGKSFHMFIY SLWFKIQRACINEKGSDRYRNAYVWAPGALKVLECHWEKYNNRYTESRSFSYIEFHCGVDGYVDNIEDLR IIEPISN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006683 |
| ORF Size | 441 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006683.5 |
| RefSeq Size | 879 bp |
| RefSeq ORF | 444 bp |
| Locus ID | 10876 |
| UniProt ID | Q14507 |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 17.6 kDa |
| Gene Summary | Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210112L3 | Lenti ORF clone of Human epididymal protein 3A (EDDM3A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210112L4 | Lenti ORF clone of Human epididymal protein 3A (EDDM3A), mGFP tagged |
CNY 5,890.00 |
|
| RG210112 | EDDM3A (tGFP-tagged) - Human epididymal protein 3A (EDDM3A) |
CNY 2,800.00 |
|
| SC303810 | EDDM3A (untagged)-Human epididymal protein 3A (EDDM3A) |
CNY 3,990.00 |
