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GRASP65 (GORASP1) (NM_031899) Human Tagged ORF Clone
CAT＃: RC210076
GORASP1 (Myc-DDK-tagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1)
ORF Plasmid: tGFP
"NM_031899" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GOLPH5; GRASP65; P65 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGGCGTCAGCGCTGAGCAGCCCGCAGGCGGCGCCGAGGGCTTCCACCTCCACGGGGTGCAGG AGAACTCCCCAGCCCAGCAGGCGGGCCTGGAGCCCTACTTTGACTTCATCATCACCATTGGGCACTCGAG GCTGAACAAGGAGAATGACACCCTGAAGGCACTACTGAAAGCCAATGTGGAGAAGCCCGTGAAGCTGGAG GTGTTCAATATGAAGACCATGAGGGTGCGCGAGGTGGAGGTGGTGCCCAGCAACATGTGGGGCGGCCAGG GCCTACTGGGTGCCAGTGTGCGCTTCTGCAGCTTCCGCAGGGCCAGTGAGCAGGTGTGGCATGTGCTGGA TGTGGAACCATCTTCACCTGCTGCCCTTGCCGGCCTGCGCCCCTACACAGACTATGTGGTTGGTTCGGAC CAGATTCTCCAGGAGTCCGAGGACTTCTTTACGCTCATCGAGTCTCATGAGGGGAAGCCCTTGAAGCTGA TGGTGTATAACTCCAAGTCAGACTCCTGCCGGGAGGTGACTGTAACTCCCAACGCAGCCTGGGGTGGAGA GGGCAGTCTGGGATGTGGCATTGGCTATGGGTATCTACACCGGATCCCAACTCAGCCCCCCAGCTACCAC AAGAAGCCACCTGGCACCCCACCACCTTCTGCTCTACCACTTGGTGCCCCACCACCTGATGCTCTACCAC CTGGACCCACCCCCGAGGACTCTCCTTCCCTGGAGACAGGTTCCAGGCAGAGTGACTACATGGAGGCCCT GCTGCAGGCACCTGGCTCCTCCATGGAGGATCCCCTTCCTGGGCCTGGGAGTCCCAGCCACAGTGCTCCA GACCCTGATGGACTTCCCCATTTCATGGAGACTCCTCTTCAGCCCCCACCTCCAGTGCAGCGAGTTATGG ACCCAGGCTTCCTGGACGTGTCGGGAATTTCTCTCTTGGACAACAGCAATGCCAGTGTGTGGCCCAGCCT GCCCTCTTCCACAGAACTGACCACCACAGCTGTCTCAACCTCAGGGCCAGAGGACATCTGCTCCAGCAGC AGTTCTCATGAGCGGGGTGGTGAGGCTACATGGTCTGGGTCAGAGTTTGAGGTCTCCTTCCTGGACAGCC CAGGTGCCCAAGCCCAGGCGGACCACCTGCCTCAGCTGACTCTTCCTGACAGTCTCACCTCTGCAGCCTC ACCAGAAGATGGGCTGTCCGCCGAGCTGCTTGAAGCTCAGGCTGAGGAGGAACCAGCAAGCACAGAGGGC CTAGATACTGGGACGGAGGCTGAGGGGCTGGACAGCCAGGCCCAGATCTCTACCACAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210076 protein sequence
Red=Cloning site Green=Tags(s) MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLE VFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSD QILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPPSYH KKPPGTPPPSALPLGAPPPDALPPGPTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPSHSAP DPDGLPHFMETPLQPPPPVQRVMDPGFLDVSGISLLDNSNASVWPSLPSSTELTTTAVSTSGPEDICSSS SSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEG LDTGTEAEGLDSQAQISTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031899 |
| ORF Size | 1320 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031899.3 |
| RefSeq Size | 3789 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 64689 |
| UniProt ID | Q9BQQ3 |
| Domains | GRASP55_65 |
| MW | 46.5 kDa |
| Gene Summary | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210076L1 | Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210076L2 | Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), mGFP tagged |
CNY 5,890.00 |
|
| RC210076L3 | Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210076L4 | Lenti ORF clone of Human golgi reassembly stacking protein 1, 65kDa (GORASP1), mGFP tagged |
CNY 5,890.00 |
|
| RG210076 | GORASP1 (tGFP-tagged) - Human golgi reassembly stacking protein 1, 65kDa (GORASP1) |
CNY 5,256.00 |
|
| SC107158 | GORASP1 (untagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1) |
CNY 3,656.00 |
|
| SC323878 | GORASP1 (untagged)-Human golgi reassembly stacking protein 1, 65kDa (GORASP1) |
CNY 3,656.00 |
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