C19orf28 (MFSD12) (NM_174983) Human Tagged ORF Clone
CAT#: RC209689
- TrueORF®
MFSD12 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3
ORF Plasmid: tGFP
"NM_174983" in other vectors (6)
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CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C19orf28; PP3501 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209689 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTGGCGCGGCTGA GCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTCACCTACCTGCTGCTCTACCT GCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTGCTGCTGCTGCTGGGCCAGGTGGCCGACGGG CTGTGCACACCGCTCGTGGGCTACGAGGCCGACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCCCGCGCA AGGCCTGGCACCTGGTCGGCACCGTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGG CTGTGGGGCGGCCACGCCCGAGTGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTT GGCTGGGCCTCCACACAGATCTCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGACCATGAGAAGG TGGAGCTCACGGCACTCAGGTATGCGTTCACCGTGGTGGCCAACATCACCGTCTACGGCGCCGCCTGGCT CCTGCTGCACCTGCAGGGCTCGTCGCGGGTGGAGCCCACCCAAGACATCAGCATCAGCGACCAGCTGGGG GGCCAGGACGTGCCCGTGTTCCGGAACCTGTCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGC TATTCCACCTGGGCACCCGGGAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTT GGCCCCTGCCACGGCCCAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTG GGCATACTGTACATGACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATGTACCTCACCT ACTCGCTCCACCTGCCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATGTACCTCAGCGGCTTCTTGTC CTCCTTCCTCATGAAGCCCATCAACAAGTGCATTGGGAGGAACATGACCTACTTCTCAGGCCTCCTGGTG ATCCTGGCCTTTGCCGCCTGGGTGGCGCTGGCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGC TGCTGGGTGCTGGCTGTGCCACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCA CACGAACAGCGGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTC ATGGCCATCCAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGCCTTTACCACT GGGCGATGGTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGTCTCTGTAGCCTCCTGCTGTG GCCGACCCGCCTGCGACGCTGGGACCGTGATGCCCGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209689 protein sequence
Red=Cloning site Green=Tags(s) MGPGPPAAGAAPSPRPLSLVARLSYAVGHFLNDLCASMWFTYLLLYLHSVRAYSSRGAGLLLLLGQVADG LCTPLVGYEADRAASCCARYGPRKAWHLVGTVCVLLSFPFIFSPCLGCGAATPEWAALLYYGPFIVIFQF GWASTQISHLSLIPELVTNDHEKVELTALRYAFTVVANITVYGAAWLLLHLQGSSRVEPTQDISISDQLG GQDVPVFRNLSLLVVGVGAVFSLLFHLGTRERRRPHAEEPGEHTPLLAPATAQPLLLWKHWLREPAFYQV GILYMTTRLIVNLSQTYMAMYLTYSLHLPKKFIATIPLVMYLSGFLSSFLMKPINKCIGRNMTYFSGLLV ILAFAAWVALAEGLGVAVYAAAVLLGAGCATILVTSLAMTADLIGPHTNSGAFVYGSMSFLDKVANGLAV MAIQSLHPCPSELCCRACVSLYHWAMVAVTGGVGVAAALCLCSLLLWPTRLRRWDRDARP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_174983 |
ORF Size | 1440 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_174983.5 |
RefSeq Size | 2149 bp |
RefSeq ORF | 1443 bp |
Locus ID | 126321 |
UniProt ID | Q6NUT3 |
Protein Families | Transmembrane |
MW | 52 kDa |
Gene Summary | Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin pigmentation (PubMed:33208952). In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin pigmentation (PubMed:33208952). Also catalyzes import of cysteine into lysosomes in non-pigmented cells (PubMed:33208952).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209689L3 | Lenti ORF clone of Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209689L4 | Lenti ORF clone of Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG209689 | MFSD12 (tGFP-tagged) - Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3 |
CNY 7,088.00 |
|
SC108337 | MFSD12 (untagged)-Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3 |
CNY 5,488.00 |
|
SC317797 | MFSD12 (untagged)-Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3 |
CNY 7,220.00 |
|
SC317798 | MFSD12 (untagged)-Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 3 |
CNY 7,220.00 |
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