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Grainyhead like protein 1 homolog (GRHL1) (NM_014552) Human Tagged ORF Clone
CAT＃: RC209683
GRHL1 (Myc-DDK-tagged)-Human grainyhead-like 1 (Drosophila) (GRHL1), transcript variant 1
ORF Plasmid: tGFP
"NM_014552" in other vectors (3)
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CNY 4,600.00
CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | grainyhead-like 1 (Drosophila); LBP-32; LBP32; LBP protein 32; leader-binding protein 32; mammalian grainyhead; MGR; TFCP2L2; transcription factor CP2-like 2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209683 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACAGGAGTACGACAACAAACGGCCAGTGTTGGTTCTTCAGAATGAAGCACTTTATCCACAGCGGC GGTCCTACACTAGTGAGGATGAGGCCTGGAAATCCTTCCTGGAAAACCCTCTCACTGCAGCGACCAAAGC GATGATGAGCATCAATGGAGATGAAGACAGCGCCGCTGCGCTGGGCCTGCTCTATGACTACTACAAGGTT CCAAGAGAGAGAAGGTCATCAACAGCAAAGCCAGAGGTGGAGCACCCTGAGCCAGATCACAGCAAAAGAA ACAGCATACCAATTGTGACAGAGCAGCCCCTCATCTCTGCTGGAGAAAACAGAGTGCAAGTACTGAAAAA TGTGCCATTTAACATTGTCCTTCCCCATGGCAACCAGCTGGGCATTGATAAGAGAGGCCATCTGACAGCT CCAGATACGACAGTCACTGTCTCCATAGCAACGATGCCTACCCACTCCATCAAGACAGAAACCCAGCCAC ATGGCTTCGCTGTGGGAATCCCCCCAGCAGTGTATCATCCTGAGCCCACTGAGCGGGTGGTGGTTTTCGA TCGGAATCTCAATACTGACCAGTTCAGCTCTGGTGCTCAAGCCCCAAATGCTCAAAGGCGAACTCCAGAC TCGACCTTCTCAGAGACCTTCAAGGAAGGCGTTCAGGAGGTTTTCTTCCCCTCGGATCTCAGTCTGCGGA TGCCTGGCATGAATTCAGAGGACTATGTTTTTGACAGTGTTTCTGGGAACAACTTTGAATATACCCTAGA AGCTTCAAAATCACTTCGACAGAAGCCAGGAGACAGTACCATGACGTACCTGAACAAAGGCCAGTTCTAT CCCATCACCTTGAAGGAGGTGAGCAGCAGTGAAGGAATCCATCATCCCATCAGCAAAGTTCGAAGTGTGA TCATGGTGGTTTTTGCTGAAGACAAAAGCAGAGAAGATCAGTTAAGGCATTGGAAGTACTGGCACTCCCG GCAGCACACCGCTAAACAAAGATGCATTGACATAGCTGACTATAAAGAAAGCTTCAACACTATCAGTAAC GTCGAGGAGATTGCGTATAACGCCATTTCCTTCACATGGGACATCAACGATGAAGCAAAGGTTTTCATCT CTGTGAACTGCTTAAGCACAGATTTCTCTTCCCAGAAGGGAGTGAAGGGGTTGCCTCTTAACATTCAAAT TGATACCTATAGTTACAACAACCGCAGCAACAAGCCTGTGCACCGGGCCTACTGCCAGATCAAGGTCTTC TGTGACAAGGGAGCTGAGCGGAAAATCAGGGATGAAGAACGAAAGCAAAGCAAAAGAAAAGTTTCTGATG TTAAAGTGCCACTGCTTCCCTCTCACAAGCGAATGGATATCACAGTTTTCAAACCCTTCATTGATCTCGA TACTCAGCCTGTCCTCTTCATTCCTGACGTGCACTTTGCCAACTTGCAGCGGGGCACTCATGTCCTTCCC ATTGCCTCTGAAGAATTGGAGGGTGAAGGCTCTGTCTTGAAAAGGGGGCCGTACGGCACAGAAGATGACT TTGCTGTCCCTCCTTCTACCAAGCTGGCCCGGATAGAAGAACCAAAGAGAGTGCTGCTCTACGTTCGAAA GGAGTCAGAAGAAGTCTTTGATGCCCTGATGCTCAAAACCCCATCTTTGAAGGGCTTGATGGAAGCTATC TCAGACAAATACGATGTTCCCCATGACAAGATTGGGAAAATATTCAAGAAGTGTAAAAAGGGGATCCTGG TGAACATGGACGACAACATTGTGAAGCATTACTCCAATGAGGACACCTTCCAGCTGCAGATTGAAGAAGC CGGGGGGTCTTACAAGCTCACCCTGACGGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209683 protein sequence
Red=Cloning site Green=Tags(s) MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKV PRERRSSTAKPEVEHPEPDHSKRNSIPIVTEQPLISAGENRVQVLKNVPFNIVLPHGNQLGIDKRGHLTA PDTTVTVSIATMPTHSIKTETQPHGFAVGIPPAVYHPEPTERVVVFDRNLNTDQFSSGAQAPNAQRRTPD STFSETFKEGVQEVFFPSDLSLRMPGMNSEDYVFDSVSGNNFEYTLEASKSLRQKPGDSTMTYLNKGQFY PITLKEVSSSEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISN VEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVF CDKGAERKIRDEERKQSKRKVSDVKVPLLPSHKRMDITVFKPFIDLDTQPVLFIPDVHFANLQRGTHVLP IASEELEGEGSVLKRGPYGTEDDFAVPPSTKLARIEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAI SDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014552 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014552.2, NP_055367.2 |
| RefSeq Size | 3610 bp |
| RefSeq ORF | 1856 bp |
| Locus ID | 29841 |
| Domains | CP2 |
| Protein Families | Transcription Factors, Transmembrane |
| MW | 70.1 kDa |
| Gene Summary | This gene encodes a member of the grainyhead family of transcription factors. The encoded protein can exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. This protein functions as a transcription factor during development. [provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209683L3 | Lenti ORF clone of Human grainyhead-like 1 (Drosophila) (GRHL1), transcript variant 1, Myc-DDK-tagged |
CNY 8,080.00 |
|
| RC209683L4 | Lenti ORF clone of Human grainyhead-like 1 (Drosophila) (GRHL1), transcript variant 1, mGFP tagged |
CNY 8,080.00 |
|
| RG209683 | GRHL1 (tGFP-tagged) - Human grainyhead-like 1 (Drosophila) (GRHL1), transcript variant 1 |
CNY 6,200.00 |
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