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WDR45 (NM_007075) Human Tagged ORF Clone
CAT＃: RC209654
- TrueORF®
WDR45 (Myc-DDK-tagged)-Human WD repeat domain 45 (WDR45), transcript variant 1
ORF Plasmid: tGFP
"NM_007075" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JM5; NBIA4; NBIA5; WDRX1; WIPI-4; WIPI4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209654 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCAACAGCCACTTCGAGGAGTGACCAGCCTGCGTTTCAACCAAGACCAAAGCTGCTTTTGCTGCG CCATGGAGACAGGTGTGCGCATCTACAACGTGGAGCCCTTGATGGAGAAGGGGCATCTGGACCACGAGCA GGTGGGCAGCATGGGCTTGGTGGAGATGCTGCACCGCTCCAACCTTCTGGCCTTGGTGGGCGGTGGTAGT AGTCCCAAGTTCTCAGAGATCTCAGCAGTGCTGATCTGGGACGATGCCCGGGAGGGCAAGGACTCCAAGG AGAAGCTGGTGCTGGAGTTCACCTTCACCAAGCCAGTGCTTTCTGTGCGCATGCGCCATGACAAGATCGT GATCGTGCTGAAGAACCGCATCTATGTGTACTCCTTCCCCGACAATCCCCGAAAGCTGTTTGAGTTTGAT ACCCGGGACAACCCCAAGGGGCTCTGTGACCTCTGCCCCAGCCTGGAGAAGCAACTGCTAGTGTTCCCGG GACACAAGTGTGGGAGTCTGCAACTTGTGGACCTGGCGAGCACAAAGCCTGGCACCTCGTCTGCTCCATT CACGATCAATGCACATCAGAGTGACATAGCCTGTGTGTCTCTAAACCAGCCAGGCACTGTAGTGGCCTCA GCCTCCCAGAAGGGTACCCTTATTCGCCTCTTTGACACACAATCCAAGGAGAAACTGGTGGAGCTGCGCC GAGGCACTGACCCTGCCACCCTCTACTGCATTAACTTCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAG TGATAAGGGTACTGTCCATATCTTTGCTCTCAAGGATACCCGCCTCAACCGCCGCTCCGCGCTGGCTCGC GTGGGCAAGGTGGGGCCTATGATTGGGCAGTACGTGGACTCTCAGTGGAGCCTGGCGAGCTTCACTGTGC CTGCTGAGTCAGCTTGCATCTGCGCCTTCGGTCGCAATACTTCCAAGAACGTCAACTCTGTCATTGCCAT CTGCGTAGATGGGACCTTCCACAAATATGTCTTCACTCCTGATGGAAACTGCAACAGAGAGGCTTTCGAC GTGTACCTTGACATCTGTGATGATGATGACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209654 protein sequence
Red=Cloning site Green=Tags(s) MTQQPLRGVTSLRFNQDQSCFCCAMETGVRIYNVEPLMEKGHLDHEQVGSMGLVEMLHRSNLLALVGGGS SPKFSEISAVLIWDDAREGKDSKEKLVLEFTFTKPVLSVRMRHDKIVIVLKNRIYVYSFPDNPRKLFEFD TRDNPKGLCDLCPSLEKQLLVFPGHKCGSLQLVDLASTKPGTSSAPFTINAHQSDIACVSLNQPGTVVAS ASQKGTLIRLFDTQSKEKLVELRRGTDPATLYCINFSHDSSFLCASSDKGTVHIFALKDTRLNRRSALAR VGKVGPMIGQYVDSQWSLASFTVPAESACICAFGRNTSKNVNSVIAICVDGTFHKYVFTPDGNCNREAFD VYLDICDDDDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007075 |
| ORF Size | 1083 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007075.3, NP_009006.2 |
| RefSeq Size | 1925 bp |
| RefSeq ORF | 1086 bp |
| Locus ID | 11152 |
| UniProt ID | Q9Y484 |
| Domains | WD40 |
| MW | 39.9 kDa |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation
,null,
Journal of neurochemistry
,PubMed ID 34837396
[WDR45]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209654L1 | Lenti ORF clone of Human WD repeat domain 45 (WDR45), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209654L2 | Lenti ORF clone of Human WD repeat domain 45 (WDR45), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209654L3 | Lenti ORF clone of Human WD repeat domain 45 (WDR45), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209654L4 | Lenti ORF clone of Human WD repeat domain 45 (WDR45), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209654 | WDR45 (tGFP-tagged) - Human WD repeat domain 45 (WDR45), transcript variant 1 |
CNY 5,256.00 |
|
| SC115710 | WDR45 (untagged)-Human WD repeat domain 45 (WDR45), transcript variant 1 |
CNY 3,656.00 |
|
| SC321010 | WDR45 (untagged)-Human WD repeat domain 45 (WDR45), transcript variant 1 |
CNY 3,656.00 |
