United States
Germany
Japan
United Kingdom
China
KLHL13 (NM_033495) Human Tagged ORF Clone
CAT＃: RC209484
KLHL13 (Myc-DDK-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1
ORF Plasmid: tGFP
"NM_033495" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BKLHD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209484 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATTGAAATGGAAAACGAGCTCTCCTGCTATCTGGAAATTCCCAGTTCCTGTGCTTAAAACATCCA GGTCAACTCCACTTTCTCCAGCATACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCT TGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACCTACACGCATCTTTACC AGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATG TGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGCTAGTGA TTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAATGTGCATTAAACTTCATGGTGTG AGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACC TTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACTTCTGTAAAGTGTTTCT CATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGCCAACACCTACAATCTAACCGAA GTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGTTCTTGA AACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTAAGCACTGTACTGAACTTGAGCT CTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAG AACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACGGTGGATTTCATGAGAA CTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAATGATGCCATATATGCAGCCAGT TATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTGCTGAGG CAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCCCATGAGTGGAAATCGTTAGCCC CCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACA GAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGATCCTCGATACAATAAA TGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGT ATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAACAAATGA ATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGGAACTGTGTATGGAGGAGTGATG TATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACA AATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTACAGTGGGAGAAAGGCT CTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTCCTAAGCTGTGAATACTAT TCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGGTCGCTG TCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATCGTTGTATGGTAGAGATAGTGCA GAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGT GCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGAGAGTCCCCTCTTTCTG CACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209484 protein sequence
Red=Cloning site Green=Tags(s) MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYISLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFT SNTHSSVVLQGFDQLRLEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGV SKVGLRKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTE VDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMK NIRFPLMTPQELINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLR QQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNK WMQVASLNEKRTFFHLSALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVM YISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYY SPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIR ACTLTVFPPEETTPSPSRESPLSAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033495 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033495.3 |
| RefSeq Size | 3991 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 90293 |
| UniProt ID | Q9P2N7 |
| Domains | BTB, Kelch |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 73.9 kDa |
| Gene Summary | This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209484L1 | Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, Myc-DDK-tagged |
CNY 7,280.00 |
|
| RC209484L2 | Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209484L3 | Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209484L4 | Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209484 | KLHL13 (tGFP-tagged) - Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1 |
CNY 6,480.00 |
|
| SC111483 | KLHL13 (untagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1 |
CNY 4,888.00 |
