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VPS11 (NM_021729) Human Tagged ORF Clone
CAT＃: RC209474
VPS11 (Myc-DDK-tagged)-Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11)
ORF Plasmid: tGFP
"NM_021729" in other vectors (7)
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CNY 6,896.00
CNY 9,790.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | END1; HLD12; hVPS11; PEP5; RNF108 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209474 representing NM_021729
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTACCTGCAGTGGCGGCGCTTCGTTTTCTTCGACAAGGAGCTGGTGAAGGAGCCGCTGAGCA ATGATGGGGCCGCTCCCGGGGCCACACCTGCTTCTGGATCCGCTGCTTCCAAGTTCCTTTGCCTCCCTCC TGGCATCACTGTCTGCGACTCAGGCCGAGGGAGCCTGGTCTTTGGAGATATGGAAGGCCAGATCTGGTTC TTGCCACGTTCCCTACAGCTTACAGGCTTCCAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGA AGCAGCACAATATTCTGGCATCTGTTGGAGAAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAA CCTGGAGAAGAGAGATGGTGGCAATCCACTCTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCA ACTGTTGTATCTTGTTTGACTGTCCATGAAAATCTCAACTTTATGGCCATTGGTTTCACAGATGGGAGTG TTACATTGAACAAAGGAGACATCACCCGGGACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTA TCCTGTAACTGGATTGGCCTTTCGCCAAGCAGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAAC GTCCAGTCCTATATAGTTTCTGGAAAAGACTACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGC GCTGCTCAGCCCTAAGTGACCCTTCTCAGGACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTT GTACCAGCCTGATGAACGTGGGCCCTGCTTCGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGA GGCTACCTTATCATTGTCTCCCGTGACCGGAAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCAC AGAGCTCCGACAAGCAGATTCTAAACATCTATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTT TGAGGATGTAGTGGATGTGCTTGCTGAGTGGGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCAC GCACTGCAGGAGAAGGACACACAGACCAAACTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGA TTAACCTTGCCAAGAGCCAGCATCTGGACAGTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCA TCTCTACAGCAAGGGCAACCACGATGGGGCTGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCA TCCTACGTGATCCGCAAGTTTCTGGATGCCCAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGC ACCGACAATCCCTGGCCAATGCCGACCATACCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAG CTCGAAGCTGGAGGAGTTCATCAAGAAAAAGAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATC AAGGTCCTCCGGCAGGCTGGCTACTACTCCCATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGT GGTACCTGAAGATCCAGCTAGAAGACATTAAGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCC TTTTGAGCAGGCAGAGAGCAACATGAAGCGCTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACA ACTCAGTTGCTGAAGGGACTTTGTACTGATTATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCC CAGGCTGCAGGGCCAACTCTGAGGAGTTCATCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTT CCTAGAGCACATGAGTGAAGTGCAGCCAGACTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGA CTGCAGAACTGGGCCCACGAGAAGGATCCACAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGC TGAAGAGTGGTCGCTTCTGTGACGTCTTTGACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGA TGGTGTCCTTTACCTTTATGAGCAGGGGAAGCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAG CAGTACCGGCAGGTCATCAGCGTGTGTGAGCGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCC TCAGCTACTTCGCTCGCAAGGAGGAGGACTGCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAA CAAGAACCTCATGCCACCTCTTCTAGTGGTGCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTC ATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGC GGCGGTACCGAGAGGAGACCACCCGTATCCGCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTT CCAAAAGACCAAGTGCAGCATCTGTAACAGTGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCAC TCCTTCCACCAACACTGCTTTGAGAGTTACTCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAA ACCGGAAGGTCATGGATATGATCCGGGCCCAGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCA GCTCAGGTGCTCCAATGACAGCTTTTCTGTGATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTG ACTCTGCTGACCGACCCTCCCACAGCCAGACTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTAC TCATGCACTCCAGGAGGGGCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209474 representing NM_021729
Red=Cloning site Green=Tags(s) MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWF LPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEP TVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTEN VQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFR GYLIIVSRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVH ALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEP SYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAI KVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQT TQLLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELR LQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHE QYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSV IRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGH SFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLRCSNDSFSVIADYFGRGVFNKL TLLTDPPTARLTSSLEAGLQRDLLMHSRRGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021729 |
| ORF Size | 2823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021729.6 |
| RefSeq Size | 3279 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 55823 |
| UniProt ID | Q9H270 |
| Domains | Clathrin, RING |
| Protein Families | Druggable Genome |
| MW | 107.6 kDa |
| Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209474L1 | Lenti ORF clone of Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), Myc-DDK-tagged |
CNY 9,296.00 |
|
| RC209474L2 | Lenti ORF clone of Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), mGFP tagged |
CNY 11,690.00 |
|
| RC209474L3 | Lenti ORF clone of Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC209474L4 | Lenti ORF clone of Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), mGFP tagged |
CNY 11,690.00 |
|
| RG209474 | VPS11 (tGFP-tagged) - Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11) |
CNY 8,496.00 |
|
| SC112921 | VPS11 (untagged)-Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11) |
CNY 6,896.00 |
|
| SC324062 | VPS11 (untagged)-Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11) |
CNY 6,896.00 |
