PRPS1L1 (NM_175886) Human Tagged ORF Clone
CAT#: RC209392
PRPS1L1 (Myc-DDK-tagged)-Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1)
ORF Plasmid: tGFP
"NM_175886" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PRPS1; PRPS3; PRPSL; PRS-III |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209392 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ACGCCGAATATCAAAATCTTCAGCGGCAGCTCCCACCAGGACTTATCCCAGAAAATTGCTGACCGCCTGG GCCTGGAGCTAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGCGTGGAAATTGATGAGAG TGTGCGTGGAGAGGATGTCTACATCGTTCAGAGTGGTTGTGGCGAAATCAACGACAGTCTAATGGAGCTT TTGATCATGATTAATGCCTGCAAGATTGCTTCAGCTAGCCGAGTTACTGCAGTCATCCCATGCTTCCCTT ATGCCCGACAGGATAAGAAGGATAAGAGCCGGTCCCCAATCTCTGCCAAGCTTGTTGCAAATATGCTCTC TATAGCAGGTGCGGATCATATCATCACCATGGACCTACATGCTTCTCAAATTCAGGGCTTTTTTGATATC CCAGTAGACAACTTGTATGCAGAGCCAACTGTCCTGAAGTGGATAAGGGAGAATATCCCTGAGTGGAAGA ACTGCATTATTGTCTCGCCAGATGCTGGTGGAGCTAAAAGAGTGACCTCCATTGCAGACCAGTTGAATGT GGACTTTGCTTTGATTCATAAAGAACGGAAGAAGGCCAATGAAGTGGACTGCATAGTGCTAGTGGGAGAT GTGAATGATCGTGTGGCTATCCTTGTAGATGACATGGCAGACACTTGTGTTACAATCTGCCTCGCAGCTG ACAAACTTCTCTCAGCTGGAGCAACCAGAGTTTATGCTATCTTGACTCATGGAATCTTTTCTGGCCCAGC CATTTCTCGCATCAACACTGCATGCTTTGAAGCAGTGGTAGTCACCAATACCATACCTCAAGATGATAAG ATGAAGCATTGCTCCAAAATACGAGTAATTGACATCTCCATGATCCTTGCAGAAGCCATAAGGAGAACTC ATAATGGGGAATCTGTTTCCTACCTGTTCAGCCATGTTCCTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209392 protein sequence
Red=Cloning site Green=Tags(s) TPNIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIDESVRGEDVYIVQSGCGEINDSLMEL LIMINACKIASASRVTAVIPCFPYARQDKKDKSRSPISAKLVANMLSIAGADHIITMDLHASQIQGFFDI PVDNLYAEPTVLKWIRENIPEWKNCIIVSPDAGGAKRVTSIADQLNVDFALIHKERKKANEVDCIVLVGD VNDRVAILVDDMADTCVTICLAADKLLSAGATRVYAILTHGIFSGPAISRINTACFEAVVVTNTIPQDDK MKHCSKIRVIDISMILAEAIRRTHNGESVSYLFSHVPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175886 |
ORF Size | 954 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175886.3 |
RefSeq Size | 1091 bp |
RefSeq ORF | 957 bp |
Locus ID | 221823 |
UniProt ID | P21108 |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Pentose phosphate pathway, Purine metabolism |
MW | 34.8 kDa |
Gene Summary | This intronless gene is specifically expressed in the testis, and encodes a protein that is highly homologous to the two subunits of phosphoribosylpyrophosphate synthetase encoded by human X-linked genes, PRPS1 and PRPS2. These enzymes convert pyrimidine, purine or pyridine bases to the corresponding ribonucleoside monophosphates. In vitro transcription/translation and site-directed mutagenesis studies indicate that translation of this mRNA initiates exclusively at a non-AUG (ACG) codon. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209392L3 | Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209392L4 | Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1), mGFP tagged |
CNY 5,890.00 |
|
RG209392 | PRPS1L1 (tGFP-tagged) - Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1) |
CNY 4,000.00 |
|
SC126124 | PRPS1L1 (untagged)-Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1) |
CNY 2,400.00 |
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