NAT2 (NM_000015) Human Tagged ORF Clone
CAT#: RC209360
NAT2 (Myc-DDK-tagged)-Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2)
ORF Plasmid: tGFP
"NM_000015" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AAC2; NAT-2; PNAT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209360 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATTGAAGCATATTTTGAAAGAATTGGCTATAAGAACTCTAGGAACAAATTGGACTTGGAAACAT TAACTGACATTCTTGAGCACCAGATCCGGGCTGTTCCCTTTGAGAACCTTAACATGCATTGTGGGCAAGC CATGGAGTTGGGCTTAGAGGCTATTTTTGATCACATTGTAAGAAGAAACCGGGGTGGGTGGTGTCTCCAG GTCAATCAACTTCTGTACTGGGCTCTGACCACAATCGGTTTTCAGACCACAATGTTAGGAGGGTATTTTT ATATCCCTCCAGTTAACAAATACAGCACTGGCATGGTTCACCTTCTCCTGCAGGTGACCATTGACGGCAG GAATTACATTGTCGATGCTGGGTCTGGAAGCTCCTCCCAGATGTGGCAGCCTCTAGAATTAATTTCTGGG AAGGATCAGCCTCAGGTGCCTTGCATTTTCTGCTTGACAGAAGAGAGAGGAATCTGGTACCTGGACCAAA TCAGGAGAGAGCAGTATATTACAAACAAAGAATTTCTTAATTCTCATCTCCTGCCAAAGAAGAAACACCA AAAAATATACTTATTTACGCTTGAACCTCAAACAATTGAAGATTTTGAGTCTATGAATACATACCTGCAG ACGTCTCCAACATCTTCATTTATAACCACATCATTTTGTTCCTTGCAGACCCCAGAAGGGGTTTACTGTT TGGTGGGCTTCATCCTCACCTATAGAAAATTCAATTATAAAGACAATACAGATCTGGTCGAGTTTAAAAC TCTCACTGAGGAAGAGGTTGAAGAAGTGCTGAAAAATATATTTAAGATTTCCTTGGGGAGAAATCTCGTG CCCAAACCTGGTGATGGATCCCTTACTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209360 protein sequence
Red=Cloning site Green=Tags(s) MDIEAYFERIGYKNSRNKLDLETLTDILEHQIRAVPFENLNMHCGQAMELGLEAIFDHIVRRNRGGWCLQ VNQLLYWALTTIGFQTTMLGGYFYIPPVNKYSTGMVHLLLQVTIDGRNYIVDAGSGSSSQMWQPLELISG KDQPQVPCIFCLTEERGIWYLDQIRREQYITNKEFLNSHLLPKKKHQKIYLFTLEPQTIEDFESMNTYLQ TSPTSSFITTSFCSLQTPEGVYCLVGFILTYRKFNYKDNTDLVEFKTLTEEEVEEVLKNIFKISLGRNLV PKPGDGSLTI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_000015 |
ORF Size | 870 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000015.3 |
RefSeq Size | 1317 bp |
RefSeq ORF | 873 bp |
Locus ID | 10 |
UniProt ID | P11245 |
Domains | Acetyltransf2 |
Protein Families | Transmembrane |
Protein Pathways | Caffeine metabolism, Drug metabolism - other enzymes, Metabolic pathways |
MW | 33.5 kDa |
Gene Summary | This gene encodes an enzyme that functions to both activate and deactivate arylamine and hydrazine drugs and carcinogens. Polymorphisms in this gene are responsible for the N-acetylation polymorphism in which human populations segregate into rapid, intermediate, and slow acetylator phenotypes. Polymorphisms in this gene are also associated with higher incidences of cancer and drug toxicity. A second polymorphic arylamine N-acetyltransferase gene (NAT1), is located near this gene (NAT2). [provided by RefSeq, Sep 2019] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Exploiting loss of heterozygosity for allele-selective colorectal cancer chemotherapy
,Rendo, V;Stoimenov, I;Mateus, A;Sjöberg, E;Svensson, R;Gustavsson, AL;Johansson, L;Ng, A;OʼBrien, C;Giannakis, M;Artursson, P;Nygren, P;Cheong, I;Sjöblom, T;,
Nat Commun
,PubMed ID 32161261
[NAT2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209360L1 | Lenti ORF clone of Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC209360L2 | Lenti ORF clone of Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2), mGFP tagged |
CNY 5,890.00 |
|
RC209360L3 | Lenti ORF clone of Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209360L4 | Lenti ORF clone of Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2), mGFP tagged |
CNY 5,890.00 |
|
RG209360 | NAT2 (tGFP-tagged) - Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2) |
CNY 4,000.00 |
|
SC116108 | NAT2 (untagged)-Human N-acetyltransferase 2 (arylamine N-acetyltransferase) (NAT2) |
CNY 2,950.00 |