eNOS (NOS3) (NM_000603) Human Tagged Lenti ORF Clone
CAT#: RC209228L3
- LentiORF®
Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, Myc-DDK-tagged
"NM_000603" in other vectors (6)
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CNY 14,960.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ECNOS; eNOS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC209228).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000603 |
ORF Size | 3609 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000603.3 |
RefSeq Size | 4345 bp |
RefSeq ORF | 3612 bp |
Locus ID | 4846 |
UniProt ID | P29474 |
Domains | flavodoxin, NAD_binding_1, FAD_binding_1, NO_synthase |
Protein Families | Druggable Genome |
Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
MW | 133.3 kDa |
Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209228 | NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 |
CNY 12,560.00 |
|
RC209228L1 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, Myc-DDK-tagged |
CNY 14,960.00 |
|
RC209228L2 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
RC209228L4 | Lenti ORF clone of Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1, mGFP tagged |
CNY 14,960.00 |
|
RG209228 | NOS3 (tGFP-tagged) - Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 |
CNY 14,160.00 |
|
SC108440 | NOS3 (untagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1 |
CNY 12,576.00 |