FKLF / KLF11 (KLF11) (NM_003597) Human Tagged Lenti ORF Clone

CAT#: RC209194L4

  • LentiORF®

Lenti ORF clone of Human Kruppel-like factor 11 (KLF11), transcript variant 1, mGFP tagged



  "NM_003597" in other vectors (4)

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CNY 5,890.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Reverse primer to sequence targets cloned in expression vectors.
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KLF11 mouse monoclonal antibody,clone OTI4H7
    • 100 ul

CNY 1,999.00
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Synonyms FKLF; FKLF1; MODY7; TIEG2; Tieg3
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC209194).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003597
ORF Size 1536 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003597.4, NP_003588.1
RefSeq Size 4051 bp
RefSeq ORF 1539 bp
Locus ID 8462
UniProt ID O14901
Domains zf-C2H2
Protein Families Transcription Factors
MW 55.1 kDa
Gene Summary The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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