PEX13 (NM_002618) Human Tagged ORF Clone

CAT#: RC209140

PEX13 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 13 (PEX13)

ORF Plasmid: DDK tGFP



  "NM_002618" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal antibody to PEX13 (peroxisome biogenesis factor 13)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms NALD; PBD11A; PBD11B; ZWS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCCAGCCGCCACCTCCCCCCAAACCCTGGGAGACCCGCCGAATTCCGGGAGCCGGACCGGGAC
CAGGACCGGGCCCCACTTTCCAATCTGCTGATTTGGGTCCTACTTTAATGACAAGACCTGGACAACCAGC
ACTTACCAGAGTGCCCCCACCTATTCTTCCAAGGCCATCACAGCAGACAGGAAGTAGCAGTGTGAACACT
TTTAGACCTGCTTACAGTTCATTTTCTTCTGGATATGGTGCCTATGGAAATTCATTTTATGGAGGCTATA
GTCCTTATAGTTATGGATATAATGGGCTGGGCTACAACCGCCTCCGTGTAGATGATCTTCCACCCAGTAG
ATTTGTTCAGCAAGCTGAAGAAAGCAGCAGGGGTGCATTTCAGTCCATTGAAAGTATTGTGCATGCATTT
GCCTCTGTCAGTATGATGATGGATGCTACCTTTTCAGCTGTCTATAACAGTTTCAGGGCTGTATTGGATG
TAGCAAATCACTTTTCCCGATTGAAAATACACTTTACAAAAGTGTTTTCAGCTTTTGCATTGGTTAGGAC
TATACGGTATCTTTACAGACGGCTACAGCGGATGTTAGGTTTAAGAAGAGGCTCTGAGAATGAAGACCTC
TGGGCAGAGAGTGAAGGAACTGTGGCATGCCTTGGTGCTGAGGACCGAGCAGCTACCTCAGCAAAATCTT
GGCCAATATTCTTGTTCTTTGCTGTTATCCTTGGTGGTCCTTACCTCATTTGGAAACTATTGTCTACTCA
CAGTGATGAAGTAACAGACAGCATCAACTGGGCAAGTGGTGAGGATGACCATGTAGTTGCCAGAGCAGAA
TATGATTTTGCTGCCGTATCTGAAGAAGAAATTTCTTTCCGGGCTGGTGATATGCTGAACTTAGCTCTCA
AAGAACAACAACCCAAAGTGCGTGGTTGGCTTCTGGCTAGCCTTGATGGCCAAACAACAGGACTTATACC
TGCGAATTATGTCAAAATTCTTGGCAAAAGAAAAGGTAGGAAAACGGTGGAATCAAGTAAAGTTTCCAAG
CAGCAACAATCTTTTACCAACCCAACACTAACTAAAGGAGCCACGGTTGCTGATTCTTTGGATGAACAGG
AAGCTGCCTTTGAATCTGTTTTTGTTGAAACTAATAAGGTTCCAGTTGCACCTGATTCCATTGGGAAAGA
TGGAGAAAAGCAAGATCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209140 protein sequence
Red=Cloning site Green=Tags(s)

MASQPPPPPKPWETRRIPGAGPGPGPGPTFQSADLGPTLMTRPGQPALTRVPPPILPRPSQQTGSSSVNT
FRPAYSSFSSGYGAYGNSFYGGYSPYSYGYNGLGYNRLRVDDLPPSRFVQQAEESSRGAFQSIESIVHAF
ASVSMMMDATFSAVYNSFRAVLDVANHFSRLKIHFTKVFSAFALVRTIRYLYRRLQRMLGLRRGSENEDL
WAESEGTVACLGAEDRAATSAKSWPIFLFFAVILGGPYLIWKLLSTHSDEVTDSINWASGEDDHVVARAE
YDFAAVSEEEISFRAGDMLNLALKEQQPKVRGWLLASLDGQTTGLIPANYVKILGKRKGRKTVESSKVSK
QQQSFTNPTLTKGATVADSLDEQEAAFESVFVETNKVPVAPDSIGKDGEKQDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002618
ORF Size 1209 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002618.4
RefSeq Size 4524 bp
RefSeq ORF 1212 bp
Locus ID 5194
UniProt ID Q92968
Domains SH3, Peroxin-13_N
Protein Families Druggable Genome
MW 44.1 kDa
Gene Summary This gene encodes a peroxisomal membrane protein that binds the type 1 peroxisomal targeting signal receptor via a SH3 domain located in the cytoplasm. Mutations and deficiencies in peroxisomal protein importing and peroxisome assembly lead to peroxisomal biogenesis disorders, an example of which is Zellweger syndrome. [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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