carabin (TBC1D10C) (NM_198517) Human Tagged ORF Clone

CAT#: RC209092

TBC1D10C (Myc-DDK-tagged)-Human TBC1 domain family, member 10C (TBC1D10C)

ORF Plasmid: DDK tGFP



  "NM_198517" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Carabin Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CARABIN; EPI64C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209092 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGGCCCTGGGGGAGGACCTGGTGCAGCCTCCCGAGCTGCAGGATGACTCCAGCTCCTTGGGGT
CCGACTCAGAGCTCAGCGGGCCTGGCCCATATCGCCAGGCCGACCGCTATGGATTCATTGGGGGCAGCTC
AGCAGAGCCAGGGCCGGGCCACCCACCTGCAGACCTCATCCGCCAACGGGAGATGAAGTGGGTGGAGATG
ACCTCGCACTGGGAGAAAACCATGTCCCGGCGGTACAAGAAGGTAAAGATGCAGTGCCGGAAAGGCATCC
CGTCTGCCCTGCGCGCCCGATGCTGGCCCCTGTTGTGTGGGGCCCATGTGTGCCAGAAGAACAGCCCTGG
CACCTATCAGGAGCTGGCAGAGGCCCCTGGAGACCCACAGTGGATGGAGACCATTGGCAGGGACCTGCAC
CGTCAATTCCCTCTGCACGAGATGTTTGTGTCGCCTCAGGGCCACGGGCAGCAGGGGCTCCTGCAGGTGC
TCAAGGCCTACACCCTGTATCGACCGGAGCAGGGCTACTGCCAGGCCCAGGGGCCCGTGGCTGCTGTGCT
GCTCATGCACCTGCCCCCAGAGGAGGCCTTCTGGTGCCTGGTGCAGATCTGTGAGGTCTACCTCCCTGGG
TACTACGGGCCCCACATGGAGGCTGTGCGGCTGGACGCCGAGGTGTTCATGGCCCTGCTGCGGCGGCTGC
TTCCGCACGTGCACAAGCACCTGCAGCAGGTGGGCGTCGGACCCCTGCTGTACCTGCCCGAGTGGTTCCT
GTGCCTCTTCGCCCGCTCCCTGCCCTTCCCCACAGTGCTGCGTGTCTGGGATGCCTTCCTCAGTGAGGGT
GCCAGAGTACTGTTCCGTGTGGGGCTGACACTGGTGCGCCTGGCGCTGGGCACTGCAGAGCAGCGAGGGG
CCTGCCCTGGCCTCCTGGAGACACTGGGAGCCCTTCGAGCCATCCCCCCCGCGCAGCTGCAGGAGGAGGC
CTTCATGTCACAGGTGCACAGCGTGGTGCTGTCAGAGCGGGACCTGCAGCGGGAGATCAAGGCCCAGCTG
GCCCAGCTGCCCGATTCCGCGCCGGGACCCCCGCCCCGGCCACAGGTCCGCCTCGCCGGGGCCCAAGCCA
TCTTTGAGGCCCAGCAGCTGGCAGGAGTGCGACGAGGCGCCAAGCCTGAGGTGCCTCGGATTGTGGTGCA
GCCCCCGGAGGAGCCCAGACCACCGCGGCGGAAACCCCAGACCCGCGGCAAGACTTTCCATGGGCTCCTG
ACTCGGGCCCGGGGCCCCCCCATCGAGGGGCCCCCCAGGCCCCAACGAGGCTCCACCTCCTTCCTGGACA
CCCGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209092 protein sequence
Red=Cloning site Green=Tags(s)

MAQALGEDLVQPPELQDDSSSLGSDSELSGPGPYRQADRYGFIGGSSAEPGPGHPPADLIRQREMKWVEM
TSHWEKTMSRRYKKVKMQCRKGIPSALRARCWPLLCGAHVCQKNSPGTYQELAEAPGDPQWMETIGRDLH
RQFPLHEMFVSPQGHGQQGLLQVLKAYTLYRPEQGYCQAQGPVAAVLLMHLPPEEAFWCLVQICEVYLPG
YYGPHMEAVRLDAEVFMALLRRLLPHVHKHLQQVGVGPLLYLPEWFLCLFARSLPFPTVLRVWDAFLSEG
ARVLFRVGLTLVRLALGTAEQRGACPGLLETLGALRAIPPAQLQEEAFMSQVHSVVLSERDLQREIKAQL
AQLPDSAPGPPPRPQVRLAGAQAIFEAQQLAGVRRGAKPEVPRIVVQPPEEPRPPRRKPQTRGKTFHGLL
TRARGPPIEGPPRPQRGSTSFLDTRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_198517
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198517.4
RefSeq Size 1752 bp
RefSeq ORF 1341 bp
Locus ID 374403
UniProt ID Q8IV04
MW 49.7 kDa
Gene Summary The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin, and is an inhibitor of both the Ras signaling pathway and calcineurin, a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromosomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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